2007, Number 1
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Vet Mex 2007; 38 (1)
Molecular characterization of Mexican isolates of chicken infectious anemia virus through restriction fragment length polymorphism
Ledesma MN, Fehervari T, Alonso R
Language: English/Spanish
References: 16
Page: 31-39
PDF size: 251.25 Kb.
ABSTRACT
Chicken infectious anemia is the main immunosuppressive disease of poultry. It is caused by a Gyrovirus. Thirty positive samples for chicken infectious anemia virus (CIAV) were identifi ed by PCR amplifi cation of a fragment of 675 bp of the amino-terminal end of the VP1 gene protein. Amplifi ed fragments were characterized by restriction fragment length polymorphism, digesting with
HaeIII,
HinfI and
HpaII endonucleases. From the 30 samples, 12 could be classifi ed into 4 previously described viral groups, whereas the 18 remaining samples formed 6 new groups. These results suggest that the virus of the chicken infectious anemia in Mexico has a wide genetic variability including worldwide distributed variants and novel local varieties.
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