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Revista Latinoamericana de Microbiología

2006, Number 3-4

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Microbiología 2006; 48 (3-4)

Achyrocline satureioides (LAM.) DC (Marcela): Antimicrobial activity on Staphylococcus spp. and immunomodulating effects on human lymphocytes

Calvo D, Cariddi LN, Grosso M, Demo MS, Maldonado AM

Language: English
References: 40
Page: 247-255
PDF size: 193.63 Kb.


ABSTRACT

Achyrocline satureioides (LAM.) DC (Compositae) is a sub-bush original from America and distributed in Europe and Africa. It is mainly used in infusions, as digestive, sedative among others and has antimicrobial and antiviral properties. A research was made into the anti-microbial activity of the A. satureioides decoction on the Staphylococcus spp strains. They were isolated from 18 patients with acne lesions and from 7 patients infected with Staphylococcus spp. (5 strains were taken from catheters and 2 from wounds). The strains were classified through biochemical tests and then were seeded in triptein-soy agar with or without decoction to observe the antibacterial activity. On the other hand, cultures of lymphocytes were made from those patients who displayed infections caused by Staphylococcus spp. and from 12 control non-infected individuals. The lymphocytes were stimulated with decoction or PHA-M. Among the expanded, CD8+ T cells, with anti-human CD8 monoclonal antibody were the outstanding ones by indirect IF. The A. satureiodes decoction inhibited 95% of the isolated Staphylococcus spp. strains and stimulated the lymphocyte expansion, of which 40% were CD8+ T cells. The A. satureiodes decoction showed anti-microbial activity and resulted to be an immunostimulating agent on CD8+ T cells, with lesser mitogenic effects than PHA-M.


REFERENCES

  1. Anesini, C., et al. “Screening of plants used in Argentine folk medicine for antimicrobial activity.” J. Ethnopharmacol. 1993; 39(2):119-28.

  2. Arredondo M., F. Blasina, C. Echeverry, A. Morquio, M. Ferreyra, J. Abin-Carriquiry, L. Lafon & F. Dajas. 2004. Cytoprotection by Achirocline satureioides (Lam) D.C. and some of its main flavonoids against oxidative stress. Journal of Ethno-pharmacology 91:13-20.

  3. Baker, J., M. Hackett & D. Simard. 1986. Variations in bacitracin susceptibility observed in Staphylococcus and Micrococcus species. Journal of Clinical Microbiology 23(5):963-964.

  4. Branson, D. 1974. Métodos en Bacteriología Clínica: Manual de Test y procedimientos. 1-256. Editorial Médica Panamericana S.A Bs As, Argentina.

  5. Caceres, A., E. Jaurequi, D. Herrera & H. Legemonn. 1998. Plants used in Guatemala for the treatment of Dermatomucosal infection. 1. Screening of 38 plants extracts for anticandidal activity. Flavor and Fragrance Journal, 13: 163-165.

  6. Carney J., J. Krenisky, R. Williamson & J. Luo. 2002. Achyrofuran, a new antihyperglycemic dibenzofuran from the South American medicinal plant Achyrocline satureioides. Journal of Natural Products. 65(2):203-205.

  7. Collins, C. & P. Lyne. 1989. Métodos Microbiológicos, 1-410. Editorial Acribia S.A. Zaragoza, España.

  8. Costerton, J., T. Marrle, K. Chene. 1985. Phenomena of bacterial adhesion. 1: 3-40. In Savage, D. & M. Fletcher (Eds.) Bacterial adhesion mechanisms and physiological significance. Plenum. Pres. New York and London.

  9. Demo, M., J. Quiñónez & V. Martin. 1999. Claves Dicotómicas para la identificación de Staphylococcus aislados de leche bovina. Revista Latinoamericana de Microbiología 41:53-57

  10. Desmarchelier, C., J. Coussio & G. Ciccia. 1998. Antioxidant and free radical scavenging effects in extract of medicinal herb Achyrocline satureioides (Lam) D.C. Brazilian Journal of Medical and Biological Research 31 (9):1163-1170.

  11. Devriese, L., K. Schleifer & G. Adegoke. 1985. Identification of coagulase-negative staphylococci from farm animals. Journal of Applied Bacteriology 58:45-55.

  12. Dimayuga, R. & S. García. 1990. Antimicrobial screening of medicinal plants from Baja California Sur México. Journal of Ethnopharmacology 31:181-192.

  13. Falk, D. & S. Guering. 1983. Differentiation of Staphylococcus and Micrococcus spp. with the Taxo A Bacitracin disk. Journal Clinical of Microbiology. 18:719-721.

  14. Geary, C. & M. Stevens. 1986. Rapid lysostaphin test to differentiate Staphylococcus and Micrococcus species. Journal of Clinical Microbiology 23:1044-1045.

  15. Heisey, R. & B. Gorman. 1992. Antimicrobial effects of plant extracts of microorganisms. Letters in Applied Microbiology, 14:136-139.

  16. Holt, J., N. Krieg, P. Sneath, J. Staley & W. Stanley. 1994. Bergey‘s Manual of Determinative Bacteriology 9th Ed. 17:532-551. Editorial Williams & Wilkins. Baltimore USA. ISBN 0-683-00603-7.

  17. Janeway, C., P. Travers, M. Walport & D. Capra. 1999. Immunobiology. The immune system in health and disease. 4th Ed Garland Publishing 4:154-155 ISBN: 0-8153-3217-3.

  18. Kadarian, C., A. Broussalis, J. Miño, P. Lopez, S. Gorzalczany & C. Acevedo. 2002. Hepatoprotective activity of Achyrocline satureioides (Lam) D.C. Pharmacological Research 45,1:57-61.

  19. Kim, T., B. Kang, D. Cho & S. Kim. 2003. Induction of interleukin-12 production in mouse macrophages by berberine, a benzodioxoloquinolizine alkaloid, deviates CD4+ T cells from a Th2 to a Th1 response. Immunology 109(3):407-414.

  20. Kloos, W. & P. Schleifer. 1975. Simplified scheme for routine identification of human Staphylococcus species. Journal of Clinical Microbiology. 1:81-88.

  21. Ko, E., S. Rho, E. Lee, Y. Seo, C. Cho, Y. Lee, B. Min, M. Shin, M. Hong & H. Bae. 2004. Traditional Korean medicine (SCRT) modulates Th1/Th2 specific cytokine production in mice CD4+ T cell. Journal of Ethnopharmacology 92(1):121-128.

  22. Kormosh N., K. Laktionov & M. Antoshechkina. 2006. Effect of a combination of extract from several plants on cell-mediated and humoral immunity of patients with advanced ovarian cancer. Phytother Res 20(5):424-5.

  23. Melnicoff, M. 1997. Immunofluorescence Methods for Microscopic Analysis, pp 294-309. In: Methods in Nonradioactive Detection. Howard, G. (Ed) Editorial Appleton & Lange.

  24. Mongelli, E., C. Desmarchelier, J. Coussio & G. Ciccia. 1995. Actividad antimicrobiana e interaccción con el ADN de plantas medicinales de la Amazonia Peruana. Revista Argentina de Microbiología. 27:199-203.ISSN: 0235-7541.

  25. Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65:55-63.

  26. Núñez, C. & J. Cantero. 2000. Las Plantas Medicinales del Sur de la Provincia de Córdoba, pp. 15-30. Edit de la Fundación UNRC. Río Cuarto, Argentina. ISBN 950-665-155-8.

  27. Owens, E. 1987. Synergistic hemolysis associated with coagulase-negative staphylococci isolated from bovine mammary glands. Journal of Clinical Microbiology 25:2037-2039.

  28. Parisi, J. 1985. Coagulase-negative staphylococci and the epidemiological typing of Staphylococcus epidermidis. Microbiological Reviews 49:126-139

  29. Polydoro, M., K. de Souza, M. Andrades, E. Da Silva, F. Bonatto, J. Heidrich, F Dal-Pizzol, E. Schapoval, V. Bassani & J. Moreira. 2004. Antioxidant, a pro-oxidant and cytotoxic effects of Achyrocline satureioides extracts. Life Sciences 74: 2815 –2826.

  30. Ratera, E. & M. Ratera. 1980. Plantas de la flora Argentina empleadas en medicina popular. Editorial Hemisferio Sur, S.A, Bs.As, Argentina. I: 5-21

  31. Rivera, F., E. Gervaz, C. Sere & F. Dajas. 2004. Toxicological studies of the aqueous extract from Achyrocline satureioides (Lam.) DC (Marcela). Journal of Ethnopharmacology 95(2-3):359-62.

  32. Romeo, A., M. Catalano & L. Lovadina. 1988. Pruebas bioquímicas mínimas para la identificación interespecie en el género Staphylococcus. Revista Argentina de Microbiología. 20 (4): 171-182.

  33. Ruffa, M., G. Ferraro, M. Wagner, M. Clacagno, R. Campos & L. Cavallaro. 2002. Cytotoxic effect of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line. Journal of Ethnopharmacology 79(3):335-339

  34. Santos, A., D. Ripoll, N. Nardi,& V. Bassani. 1999. Immunomodulatory effect of Achyrocline satureioides (LAM.) D.C. aqueous extracts. Phytotherapy Research 13(1): 65-66.

  35. Simoes, C., E. Schenkel, L. Bauer & A. Langeloh. 1988. Pharmacological investigations on Achyrocline satureioides (LAM.) DC. Compositae. Journal of Ethnopharmacology 22(3): 281-293.

  36. Smibert, M. & N. Krieg. 1981. General Characterization, 20:409-443In Gerahardt, G. (Ed.). Manual of Methods for General Bacteriology. American Society for Microbiology Washington.

  37. Smith, D., N. Conant, J. Duerman & J. Beard. 1998. Zinsser Hans Microbiología, 23: 554-573 Editorial Médica Panamericana. Bs. As., Argentina. ISBN: 950-06-2615-2-84-7903-171-9.

  38. Tuchscherer, M., E. Kanitz, W. Otten & A. Tuchscherer. 2002. Effects of prenatal stress on cellular and humoral immune responses in neonatal pigs. Veterinary Immunology and Immunopathology 86:195-203.

  39. Wagner, H., A Proksch, I. Riess-Maurer, A. Vollmar, S. Odenthal, H. Stuppner, K. Jurcic, M. Le Turdu & J. Fang. 1985 Immunostimulating action of polysaccharides (heteroglycans) from higher plants. Arzneimittelforschung. 35(7):1069-1075.

  40. Zanon S., F. Ceriatti, M. Rovera, L. Sabini & B. Ramos. 1999. Search for antiviral activity of certain Medicinal Plants from Córdoba, Argentina. Revista Latinoamericana de Microbiología 41(2):59-62.




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