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2022, Number 1

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Rev Cubana Plant Med 2022; 27 (1)

Phytochemical components and antibacterial activity of ethanolic extract of Cnicus Benedictus Linneau, Asteraceae

Vieira VV, Nunes SC, França OJF

Language: Portugués
References: 15
Page:
PDF size: 190.51 Kb.


ABSTRACT

Introduction: Cnicos benedictus Linneau, popularly known as thistle, is a species used in folk medicine to treat a variety of diseases, although without scientific evidence of its effectiveness.
Objective: To evaluate the antibacterial activity and phytochemical screening of the ethanolic extract of Cnicos benedictus Linneau.
Methods: The antibacterial activity of the ethanolic extract of Cnicos benedictus Linneau was performed using the disc diffusion method. Minimum inhibitory concentration values were determined using the broth microdilution test in accordance with NCCLS standards. Phytochemical screening of secondary metabolites was performed according to chromogenic chemical reactions or precipitation methodologies.
Results: The results showed that the raw extract presented a phytochemical profile composed of alkaloids, steroids, phenols, flavonoids, tannins, triterpenoids and saponins. The minimum inhibitory concentrations of the extract ranged from 32.0 to 205.0 μg mL-1 in Gram-positive bacteria. However, no inhibitory activity was observed in the growth of the Gram-negative bacterial strains analyzed.
Conclusions: The raw ethanolic extract of Cnicos benedictus Linneau has antimicrobial potential against Gram-positives, which makes it an important alternative for the bioprospecting of new drugs with antibiotic properties.


REFERENCES

  1. Ying P, Yuqing J, Ali Z, Bin L, Kaiqiang Z, Fei L, et al. Two new sesquiterpene lactone glycosides from Cnicus benedictus. Natural Product Research. 2017 [acceso: 30/12/2021];31(19):2211-7. Disponible en: https://pubmed.ncbi.nlm.nih.gov/28287272/

  2. Paun G, Neagu E, Moroeanu V, Albu C, Savin S, Lucian RG. Chemical and bioactivity evaluation of Eryngium planum and Cnicus benedictus polyphenolic-rich extracts. BioMed Research Internat. 2019 [acceso: 30/12/2021];12:1-10. Disponible en: https://www.hindawi.com/journals/bmri/2019/3692605/

  3. Gomes EM, Firmino AV, Pena RC, Almeida SS. Efeito inibitório in vitro de extratos de Cinnamomum zeylanicum Blume no controle de Cylindrocladium candelabrum. Ciência Florestal. 2018 [acceso: 30/12/2021];28(4):1559-67. Disponible en: https://www.scielo.br/j/cflo/a/jPMjdN8SkFNpxVptH3xwp4k/?format=pdf&lang=pt

  4. Bona EA, Pinto FG, Fruet TK, Jorge TC, Moura AC. Comparação de métodos para avaliação da atividade antimicrobiana e determinação da concentração inibitória mínima (CIM) de extratos vegetais aquosos e etanólicos. Arquivos Instituto Biológico. 2014 [acceso: 30/12/2021];81(3):218-25. Disponible en: https://www.scielo.br/j/aib/a/mwDLMCbVGPRvH4gdFNJMV4F/?format=pdf&lang=pt

  5. Vieira CB, Orlanda JF. Atividade antioxidante e fotoprotetora doextrato etanólico de Ocimum gratissimum L. (alfavaca, Lamiaceae). Rev Cubana Plant Med. 2018 [acceso: 30/12/2021];23(3):1-15. Disponible en: http://revplantasmedicinales.sld.cu/index.php/pla/article/view/626/319

  6. Vale VV, Orlanda JF. Atividade antimicrobiana do extrato bruto etanólico das partes aéreas de Euphorbia tirucalli Linneau (Euphorbiaceae). Scientia Plena. 2011 [acceso: 30/12/2021];7(4):1-6. Disponible en: https://www.scientiaplena.org.br/sp/article/view/341

  7. Matos FJ. Introdução a fitoquímica experimental. 2.ed. Fortaleza: UFC; 1997.

  8. Orlanda JF; Nascimento AR. Chemical composition and antibacterial activity of Ruta graveolens L. (Rutaceae) volatile oils, from São Luís, Maranhão, Brazil. South African J Botany. 2015 [acceso: 30/12/2021];99:103-6. Disponible en: https://www.sciencedirect.com/science/article/pii/S0254629915002628

  9. Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum species. J Agricultural Food Chemistry. 2001 [acceso: 30/12/2021];49:4168-70. Disponible en: https://www.researchgate.net/publication/11789056_Composition_and_Antimicrobial_Activity_of_the_Essential_Oils_of_Two_Origanum_Species

  10. Simões CO, Schenkel EP, Gosmann G, Mello JC, Mentz LA, Petrovick PR. Farmacognosia: da planta ao medicamento. 6. ed. Porto Alegre: UFSC; 2017.

  11. Matte AK, Deak AR, Mata PTG. Triagem fitoquímica e avaliação da atividade antibacteriana de extratos das flores de Sambucus nigra L. (Caprifoliaceae). Rev Bras Plant Med. 2015 [acceso: 30/12/2021];17(4):1049-54. Disponible en: https://www.scielo.br/j/rbpm/a/PtQDn7dDhcvMcK5cRzm9k4g/abstract/?lang=pt

  12. Ventura PA, Jesus JP, Nogueira JR, Galdos AC. Análise fitoquímica e avaliação da susceptibilidade antimicrobiana de diferentes tipos de extratos de Plantago major L. (Plantaginaceae). Infarma-Ciências Farmacêuticas. 2016 [acceso: 30/12/2021];28(1):33-9. Disponible en: https://www.researchgate.net/publication/299652268_Analise_fitoquimica_e_avaliacao_da_susceptibilidade_antimicrobiana_de_diferentes_tipos_de_extratos_de_Plantago_major_L_Plantaginaceae

  13. Fernandes EE, Pulwale AV, Patil GA, Moghe AS. Probing regenerative potential of Moringa oleifera aqueous extracts using in vitro cellular assays. Pharmacognosy Research. 2016 [acceso: 30/12/2021];8(4):231-7. Disponible en: https://pubmed.ncbi.nlm.nih.gov/27695260/

  14. Baratto L, LangKL, Vanz DC, Reginatto FH, Oliveira JB, Falkenberg M. Investigação das atividades alelopática e antimicrobiana de Mikania laevigata (Asteraceae) obtida de cultivos hidropônico e tradicional. Rev Bras Farmacog. 2008 [acceso: 30/12/2021];18(4):577-82. Disponible en: https://www.scielo.br/j/rbfar/a/jfbCBbzWWBKK6nLBXZvMmQH/?lang=pt

  15. Gaspari JF, Virtuoso S, Davet A, Cunico MM, Miguel MD, Miguel OG, et al. Atividade antibacteriana e antifúngica de extratos etanólicos de Aster lanceolatus Willd., Asteraceae. Rev Bras Farmacog. 2006 [acceso: 30/12/2021];16(1):83-7. Disponible en: https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32789/1/atividade-antibacteriana-e-antifungica-de-extratos-etanolicos.pdf




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