García CS, Herrera OA, Rojas DHU, Olamendi PM, Xibille FDX, Sánchez AMÁ

Language: Spanish
References: 16
Page: 53-57
PDF size: 209.58 Kb.

ABSTRACT

Background. Dried blood spots (DBS) are alternative to identify diseases. The use of DBS to detect antibodies IgG and IgA against SARS-COV-2 was evaluated by ELISA.
Material and method. A biobank of paired plasma and dbs samples was formed. Two volumes for dbs elution, 250 µl and 400 µl, were evaluated for antibody by commercial tests. Sensitivity, specificity, positive predictive value, negative predictive value, Sperman correlation, and kappa concordance were calculated, and Bland-Altman graphs were performed.
Results. The sensitivity and specificity for igg antibodies with dbs eluted in 250 µl was 98.9 and 100%, and with 400 µl it was 97.4 and 100%. The igg indices showed a greater dispersion from the value of 6. The present study is one of the first to report a validation of iga with DBS, a sensitivity and specificity of 100 and 97.8% was found, as well as a greater dispersion at from the index of 12.
Conclusion. It is feasible to use DBS to detect IgG and IgA antibodies against SARS-COV-2 in epidemiological studies, since they show good concordance and correlation with plasma.

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