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2022, Number 1

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VacciMonitor 2022; 31 (1)

Comparative efficacy of different Carbopol concentrations as a stabilizer of live attenuated sheep pox virus vaccine against lumpy skin disease

Ibrahim AI, Rady DI, Abdo ER, El-Kholy AA

Language: English
References: 19
Page: 34-42
PDF size: 376.34 Kb.


ABSTRACT

In Egypt, the lyophilized live attenuated sheep pox virus vaccine has been used for the vaccination of cattle against lumpy skin disease virus to control its economic impact on livestock industry. In this endeavor, we validate the efficacy of CarbopolŪ as a stabilizer and adjuvant to enhance immunogenicity of such a heterologous sheep pox virus vaccine against lumpy skin disease. Lyophilization of sheep pox virus vaccine stabilized with CarbopolŪ produced better physical and antigenic properties than freeze-drying with lactalbumin/sucrose stabilizer; this was manifested by superior disc uniformity, thermo-stability at 37oC, and less reduction in virus titer. Immunization of calves' groups with variable sheep pox vaccine doses containing different CarbopolŪ concentrations revealed that 103.5 TCID50 of sheep pox virus vaccine enclosing 0.5% CarbopolŪ is the field dose of choice. Moreover, it induced protective serum neutralizing index of 2.5 and a ELISA S/P ratio of 36, by the 4th week post vaccination. Besides, the inclusion of 0.5% CarbopolŪ in formulation of the sheep pox virus vaccine was safe in bovines and enhanced cellular immune response to lumpy skin disease virus, as evidenced by increased T cell proliferation. Hence, it is recommended to use CarbopolŪ as 0.5% in preparation of live attenuated sheep pox virus vaccine to confer better protection against lumpy skin disease virus infection.


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