medigraphic.com
ISSN 1561-3054 (Electronic)

2022, Number 1

<< Back Next >>

Rev Cubana Med Trop 2022; 74 (1)

Bacterioma in Mammary Nodules and Their Morphological Effects on Breast Cancer Cell Line

Pertuz YK, Sierra HA, Díaz YI, Escobar PJ, De Nubbila E

Language: Spanish
References: 42
Page:
PDF size: 362.61 Kb.


ABSTRACT

Introduction: No bacterium has been reported as causative of breast cancer, but the presence of different species that are related in a beneficial or detrimental way.
Objective: To identify the microbiota present in breast nodules and its morphological effects on the MCF-7 cell line.
Methods: Exploratory, experimental study of a population of 57 women with breast nodules, operated for needle biopsy in a diagnostic center. Of these, 17 samples were chosen by non-probabilistic sampling for metagenomic analysis. Infection was performed with MCF-7 cells and Staphylococcus saprophyticus at MOI of 1:1, 5:1 and 10:1 (48 hours of exposure).
Results: Of the 57 samples taken, only 7 yielded positive results (12.28%) and the rest (50) had no bacterial growth. The following microbiota was obtained by metagenomics: Proteobacteria (47%), Escherichia (9.4%) and Yokenella (8.2%), among others. Controls were negative. Only two patients tested positive for cancer, and S. saprophyticus was the common species. In the infection, morphological changes were evidenced from MOI 5:1.
Conclusion: The bacteriome extracted from the nodules of a female population is mostly endogenous flora of the mammary glands.


REFERENCES

  1. Bhatt AP, Redinbo MR, Bultman SJ. The role of the microbiome in cancer development and therapy. CA Cancer J Clin. 2017 [Acceso 15/01/2021];67 ( 4):326-44. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5530583/

  2. Mikó E, Kovács T, Sebő É, Tóth J, Csonka T, Ujlaki G, et al. Microbiome -Microbial Metabolome- Cancer Cell Interactions in Breast Cancer -Familiar, but Unexplored. Cells. 29 de marzo de 2019 [Acceso 18/03/2021];8(4). Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523810/

  3. Chen J, Douglass J, Prasath V, Neace M, Atrchian S, Manjili MH, et al. The microbiome and breast cancer: a review. Breast Cancer Res Treat. 2019 [Acceso 15/01/2021];178(3):493-6. Disponible en: https://link.springer.com/article/10.1007/s10549-019-05407-5

  4. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 [Acceso 10/12/2020];68(6):394-424. Disponible en: Disponible en: https://acsjournals.onlinelibrary.wiley.com/doi/full/10.3322/caac.21492

  5. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2020 [Acceso 18/03/2021]. Disponible en: https://acsjournals.onlinelibrary.wiley.com/doi/abs/10.3322/caac.21660

  6. Urbaniak C, Cummins J, Brackstone M, Macklaim JM, Gloor GB, Baban CK, et al. Microbiota of Human Breast Tissue. Macfarlane GT, ed. Appl Environ Microbiol. 2014 [Acceso 18/12/2020];80(10):3007. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018903/

  7. Xuan C, Shamonki JM, Chung A, DiNome ML, Chung M, Sieling PA, et al. Microbial Dysbiosis is Associated with Human Breast Cancer. Takabe K, ed. PLoS ONE. 2014 [Acceso 18/12/2020];9(1):e83744. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885448/

  8. Donnet-Hughes A, Perez PF, Doré J, Leclerc M, Levenez F, Benyacoub J, et al. Potential role of the intestinal microbiota of the mother in neonatal immune education. Proc Nutr Soc. 2010 [Acceso 18/12/2020];69(3):407-15. Disponible en: Disponible en: https://www.cambridge.org/core/journals/proceedings-of-the-nutrition-society/article/potential-role-of-the-intestinal-microbiota-of-the-mother-in-neonatal-immune-education/C780F421FFBA3653238EF9E7C23EAA08

  9. Jost T, Lacroix C, Braegger CP, Rochat F, Chassard C. Vertical mother-neonate transfer of maternal gut bacteria via breastfeeding: Mother-neonate bacterial transfer. Environ Microbiol. 2014 [Acceso 09/01/2021];16(9):2891-904. Disponible en: Disponible en: https://www.researchgate.net/publication/256608536_Vertical_mother-neonate_transfer_of_maternal_gut_bacteria_via_breastfeeding

  10. Wang H, Altemus J, Niazi F, Green H, Calhoun BC, Sturgis C, et al. Breast tissue, oral and urinary microbiomes in breast cancer. Oncotarget. 2017 [Acceso 19/12/2020];8(50):88122-38. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5675698/

  11. Hunt KM, Foster JA, Forney LJ, Schütte UME, Beck DL, Abdo Z, et al. Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human Milk. Plos One. 2011 [Acceso 19/12/2020];6(6):e21313. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3117882/

  12. Rescigno M, Urbano M, Valzasina B, Francolini M, Rotta G, Bonasio R, et al. Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria. Nat Immunol. 2001 [Acceso 27/01/2021];2(4):361-7. Disponible en: Disponible en: https://pubmed.ncbi.nlm.nih.gov/11276208/

  13. Parida S, Sharma D. The power of small changes: Comprehensive analyses of microbial dysbiosis in breast cancer. Biochim Biophys Acta BBA - Rev Cancer. 2019 [Acceso 22/01/2021];1871(2):392-405. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8769497/

  14. Chan AA, Bashir M, Rivas MN, Duvall K, Sieling PA, Pieber TR, et al. Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors. Sci Rep. 2016[Acceso 18/03/2021];6(1):28061. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4914981/

  15. Hieken TJ, Chen J, Hoskin TL, Walther-Antonio M, Johnson S, Ramaker S, et al. The Microbiome of Aseptically Collected Human Breast Tissue in Benign and Malignant Disease. Sci Rep. 2016 [Acceso 01/12/2020];6(1):30751. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971513/

  16. Urbaniak C, Gloor GB, Brackstone M, Scott L, Tangney M, Reid G. The Microbiota of Breast Tissue and Its Association with Breast Cancer. Appl Environ Microbiol. 2016 [Acceso 18/12/2020];82(16):5039-48. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4968547/

  17. Meng S, Chen B, Yang J, Wang J, Zhu D, Meng Q, et al. Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy. Front Oncol. 2018;8:312. DOI: https://doi.org/10.3389/fonc.2018.00318

  18. Costantini L, Magno S, Albanese D, Donati C, Molinari R, Filippone A, et al. Characterization of human breast tissue microbiota from core needle biopsies through the analysis of multi hypervariable 16S-rRNA gene regions. Sci Rep . 2018 [Acceso 23/11/2020];8(1):16893. Disponible en: Disponible en: https://www.nature.com/articles/s41598-018-35329-z

  19. Metzker ML. Sequencing technologies -the next generation. Nat Rev Genet. 2010 [Acceso 23/11/2020];11(1):31-46. Disponible en: Disponible en: https://www.nature.com/articles/nrg2626

  20. QIAamp PowerFecal Pro DNA Kit - QIAGEN Online Shop. [Acceso 19/03/2021]. Disponible en: Disponible en: https://www.qiagen.com/co/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/qiaamp-powerfecal-pro-dna-kit/?clear=true#orderinginformation

  21. Vesty A, Biswas K, Taylor MW, Gear K, Douglas RG. Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities. Plos One . 2017 [Acceso 18/01/2021];12(1):e0169877. Disponible en: Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169877

  22. Ning J, Liebich J, Kästner M, Zhou J, Schäffer A, Burauel P. Different influences of DNA purity indices and quantity on PCR-based DGGE and functional gene microarray in soil microbial community study. Appl Microbiol Biotechnol. 2009 [Acceso 23/11/2020];82(5):983-93. Disponible en: https://link.springer.com/article/10.1007/s00253-009-1912-0

  23. Herlemann DP, Labrenz M, Jürgens K, Bertilsson S, Waniek JJ, Andersson AF. Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME J. 2011 [Acceso 23/11/2020];5(10):1571-9. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176514/

  24. Kuczynski J, Stombaugh J, Walters WA, González A, Caporaso JG, Knight R. Using QIIME to analyze 16S rRNA gene sequences from microbial communities. Curr Protoc Bioinformatics. Dec 2011 ; Chapter Unit 10.7. DOI: https://doi.org/10.1002/0471250953.bi1007s36

  25. Fagundo-Sierra R, Cerros-Santos MA, Pérez-Jáuregui J. Evaluación del instrumento automatizado Phoenix en la identificación y antibiograma de bacterias de origen clínico. Bioquimia. 2007 [Acceso 19/03/2021];32(2):39-48. Disponible en: Disponible en: https://www.medigraphic.com/pdfs/bioquimia/bq-2007/bq072b.pdf

  26. Corrales Ramírez L, Sánchez Leal L. Evaluación de la congelación para conservación de especies autóctonas bacterianas. Nova. 2005 [Acceso 11/12/2020];3(4):21. Disponible en: Disponible en: https://hemeroteca.unad.edu.co/index.php/nova/article/view/333/1208

  27. SOP Thawing, Propagating and Cryopreserving of NCI-PBCF-HTB22 (MCF-7). 1, March 2012; Version 1.5. [Acceso 19/03/2021]. Disponible en: Disponible en: https://www.downloads/NCI-PBCF-HTB26_MDA-MB-231_SOP-508.pdf

  28. Letourneau J, Levesque C, Berthiaume F, Jacques M, Mourez M. In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells. J Vis Exp. 2011 May;(51):2783. DOI: https://doi.org/10.3791/2783.

  29. Shabram P, Aguilar-Cordova E. Multiplicity of Infection/Multiplicity of Confusion. Mol Ther. 2000 [Acceso 19/03/2021];2(5):420-1. Disponible en: https://www.cell.com/action/showPdf?pii=S1525-0016%2800%2990212-0

  30. Knipe DM, Howley PM. Fields’ Virology. Philadelphia: Lippincott Williams & Wilkins; 2007. 3116 p.

  31. Gutiérrez LA, Agudelo DA. Control del crecimiento in vitro sobre cepas Gram positivas y Gram negativas productoras de mastitis. Rev LASALLISTA Investig. 2009 [Acceso 11/03/2021];6(1):67-74. Disponible en: Disponible en: http://www.scielo.org.co/pdf/rlsi/v6n1/v6n1a09.pdf

  32. AnimCellCulture_Guide.pdf [Internet]. [Acceso 19/03/2021]. Disponible en: Disponible en: https://www.atcc.org/resources/culture-guides/animal-cell-culture-guide

  33. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 [Acceso 2/03/2021];65(1):55-63. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/0022175983903034?via%3Dihub

  34. Thyagarajan S, Zhang Y, Thapa S, Allen MS, Phillips N, Chaudhary P, et al. Comparative analysis of racial differences in breast tumor microbiome. Sci Rep. 2020 [Acceso 2/03/2021];10(1):14116. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445256/

  35. Tyson GW, Chapman J, Hugenholtz P, Allen EE, Ram RJ, Richardson PM, et al. Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature. 2004 [Acceso 22/02/2021];428(6978):37-43. Disponible en: Disponible en: https://www.nature.com/articles/nature02340

  36. Lu X, Zhang X-X, Wang Z, Huang K, Wang Y, Liang W, et al. Bacterial Pathogens and Community Composition in Advanced Sewage Treatment Systems Revealed by Metagenomics Analysis Based on High-Throughput Sequencing. Plos One. 2015 [Acceso 22/02/2021];10(5):e0125549. Disponible en: Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0125549

  37. Altarejos PI. Comparación de estrategias metagenómicas en muestras ambientales de biofilms. Centro Superior de Investigación en Salud Pública (CSISP) - Fundación para la Investigación Sanitaria y Biomédica en la Comunidad Valenciana (FISABIO). Valencia; 2015. [Trabajo Final de Grado]. Disponible en: https://m.riunet.upv.es/bitstream/handle/10251/56314/IZQUIERDO-Metagen%C3%B3

  38. Streit WR, Schmitz RA. Metagenomics -the key to the uncultured microbes. Curr Opin Microbiol. 2004 [Acceso 22/02/2021];7(5):492-8. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S1369527404000967?via%3Dihub

  39. Kofteridis DP, Valachis A, Koutsounaki E, Maraki S, Mavrogeni E, Economidou FN, et al. Skin and Soft Tissue Infections in Patients with Solid Tumours. Sci World J. 2012. DOI: https://doi.org/10.1100/2012/804518

  40. Alekseeva L, Rault L, Almeida S, Legembre P, Edmond V, Azevedo V, et al. Staphylococcus aureus-Induced G2/M Phase Transition Delay in Host Epithelial Cells Increases Bacterial Infective Efficiency. Plos One . 2013 [Acceso 19/03/2021];8(5):e63279. Disponible en: Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662696/

  41. Szabados F, Kleine B, Anders A, Kaase M, Sakinç T, Schmitz I, et al. Staphylococcus saprophyticus ATCC 15305 is internalized into human urinary bladder carcinoma cell line 5637. FEMS Microbiol Lett. 2008 [Acceso 18/03/2021];285(2):163-9. Disponible en: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1574-6968.2008.01218.x?sid=nlm%3Apubmed

  42. O´Connor H, MacSharry J, Bueso YF, Lindsay S, Kavanagh EL, Tangney M, et al. Resident Bacteria in Breast Cancer Tissue: Pathogenic Agents or Harmless Commensals? Discov Med. Sep 2018 [Acceso 21/02/2021];26(142):93-102. Disponible en: Disponible en: https://www.discoverymedicine.com/Hugh-OConnor/2018/09/resident-bacteria-in-breast-cancer-tissue-pathogenic-agents-or-harmless-commensals/




2020     |     www.medigraphic.com