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Órgano oficial de la Universidad Autónoma de Sinaloa

2023, Number 3

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Rev Med UAS 2023; 13 (3)

Induction of mammary tumors by orthotopic transplantation of 4T1 cells in BALB/c mice of different parental inbred line: pilot study

Loera-Heras Z, Anaya-Ruiz M, Gutiérrez-Saldívar J, Astiazaran-García H

Language: Spanish
References: 29
Page: 250-259
PDF size: 539.33 Kb.


ABSTRACT

Objective. Develop mammary tumors by orthotopic transplantation of 4T1 cells in mice of the same BALB/c strain but a different parental inbred line. Materials and methods. An experimental study was carried out. 4T1 cells (5x104) were transplanted in the fourth mammary fat pad of female BALB/c mice from inbred line A (IL-A n=4) and inbred line B (IL-B n=3). The day of transplantation is called the first day (D0), on days’ post-transplantation D7, D14, D21 and D28 tumor growth was assessed using an electronic caliper. Statistical design with t student test. Results. On D28 both inbred develop solid tumors with a volume 1007.9± 335.6 mm3 and 994.0±330.7 mm3 no significant differences were observed P ›0.05. The histological grade in both inbred lines was 3 and an increase in spleen size 291.6±44.3 mm2 (IL-A) and 282±18.8 mm2 (IL-B) no significant differences were observed P ›0.05. Conclusions. Both inbred lines (A and B) of the BALB/c strain developed solid tumors of similar size, appearance, and histopathology.


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