2023, Number 1
Cloning and recombinant expression of elongation factor-1α of Leishmania infantum
Language: Spanish
References: 7
Page: 99-103
PDF size: 583.16 Kb.
ABSTRACT
Introduction. In visceral leishmaniasis (VL), in old world caused by Leishmania donovani, elongation factor-1α (EF-1α) is an antigen-activator of SHP-1 phosphatase that inhibits the leishmanicidal activity of macrophages. However, the relevance of EF-1α from L. infantum in VL of the new world is still unknown.Objective. Obtain the recombinant protein of EF-1α from L. infantum (rEFLi).
Material and methods. The EFLi gene was cloned, sequenced, and expressed as recombinant protein in a prokaryotic system. rEFLi was purified and its immunogenicity was verified.
Results. The EFLi gene has 1350 pb, with 25 nucleotide differences, 10 amino acid differences and without structural differences (3D) compared to EFLd. rEFLi (50 kDa), was recognized by sera from mice immunized with its homologous genetic construction.
Conclusion. Our evidence suggests that rEFLi can be evaluated as a biotechnological vaccine alone or in combination, for the prevention of VL.
REFERENCES
53-64. https://doi.org/10.1002/prot.212785. López-López K, Apodaca-Medina A, León-SicairosCR, Murúa-López CC, Ortiz-Navarrete VF, Chávez-Ontiveros J, et al. Cloning and recombinant expressionof Elongation Factor-1a of Leishmania mexicana. VectorBorne Zoon Dis. 2018 18(12): 709-712. https://doi.org/10.1089/vbz.2018.2347
Iborra S, Soto M, Carrión J, Nieto A, Fernández E, AlonsoC, et al. The Leishmania infantum acidic ribosomalprotein P0 administered as a DNA vaccine confersprotective immunity to Leishmania major infection inBALB/c mice. Infect Immun. 2003 71(11): 6562-6572.https://doi.org/10.1128/IAI.71.11.6562-6572.2003