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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2023, Number 1

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Rev Biomed 2023; 34 (1)

Characterization of the in vitro metacyclogenesis process of Leishmania (Leishmania) mexicana promastigotes Introduction

Chacón-Vargas KF, Corral-Ruiz G, Sánchez-Torres LE

Language: Spanish
References: 33
Page: 13-21
PDF size: 251.58 Kb.


ABSTRACT

Introduction. Leishmaniasis is a spectrum of vector-borne diseases caused by protozoa of the genus Leishmania sp. In Mexico, species of the Leishmania (L.) mexicana complex are endemic and cause cutaneous leishmaniasis. During the development of the parasite in the vector and in culture, the process of metacyclogenesis has been described, which consists of morphological and biochemical changes for the transformation of procyclic promastigotes into metacyclic promastigotes.
Objective. To characterize the process of metacyclogenesis in axenic cultures of Leishmania (L.) mexicana.
Materials and methods. In promastigote cultures of L. (L.) mexicana, different parameters were evaluated daily for 15 days to determine changes over time. Cellularity, morphology, and cell viability parameters were analyzed by optical microscopy and flow cytometry.
Results. In the growth curve, three stages were identified. a) Logarithmic phase, from day 0 to 6, procyclic promastigotes predominate, they are larger, viable, metabolically active and have a high replication rate; b) stationary phase, from day 7 to 10, metacyclic promastigotes are abundant, they are thin and elongated, there is a proportion of the parasites with loss of viability; c) death phase, from day 11, there is a decrease in cell density and the remaining parasites present apoptotic characteristics.
Conclusion. The characterization of the growth curve of L. (L.) mexicana allows to identify easily, quickly, and at low-cost, the time when there is a higher proportion of metacyclic promastigotes in culture, reducing the variability in the results of in vitro and in vivo experiments.


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