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Órgano oficial de la Universidad Autónoma de Sinaloa

2022, Number 3

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Rev Med UAS 2022; 12 (3)

Expression profile of miR-34, miR-106 and miR-184 microRNAs in multifactorial dry eye disease

Rodríguez-de la Vega AT, Alvarez-Félix JR, Guadrón-Llanos AM, Romo-García E, Magaña-Gómez JA, Angulo-Rojo CE

Language: Spanish
References: 18
Page: 206-222
PDF size: 277.59 Kb.


ABSTRACT

Dry eye disease is a multifactorial disease of the ocular surface and tear, which causes a clinical presentation of ocular symptoms and signs, with fluctuating vision impairment and an unstable tear film with potential damage to the ocular surface. MicroRNAs represent a recently discovered family made up of small RNAs, 18 to 25 nucleotides in length. Objective: to characterize the expression pattern of miR-34, miR-106 and miR-184 microRNAs in multifactorial dry eye disease, to evaluate their potential as biomarkers. Material and methods: observational, cross-sectional, descriptive and prospective study in patients with dry eye disease compared to healthy patients, where the expression of three microRNAs was quantified. Patients included in this study underwent an ophthalmological examination and clinical evaluation, blood and tear sampling for analysis and quantification of miRNAs by RT-PCR technique, with the Pfaffl method. Results: overexpression of miR-106 was found in serum, with a rate of change 2 times more compared to the control group. And overexpression of miR-184 in tears, with a rate of change 3 times more, compared to the control group, both statistically significant. Conclusion: our results suggested that miR-106 and miR-184 could be involved in the pathophysiology of dry eye disease, systemically (miR-106) or locally (miR184). However, it is difficult to define the role of microRNAs in the pathophysiology of dry eye disease, the main reason why larger studies are needed for a better exploration of these molecular pathways, and thus validate their role as early diagnostic biomarkers for the detection of dry eye disease.


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