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2021, Number 4

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Rev Med UAS 2021; 11 (4)

Autoimmune Etiology of Dry Eye in Patients with Sjögren's Syndrome

Aragón-Arreola PA, Álvarez-Félix JR, Angulo-Rojo CE, Romo-García E

Language: Spanish
References: 17
Page: 320-328
PDF size: 129.58 Kb.


ABSTRACT

Dry eye is a multifactorial pathology caused by various pathophysiological mechanisms depending on the patient's condition. Inflammatory pathologies such as Sjögren's Syndrome is an exocrinopathy caused by an increase in pro-inflammatory mediators. MicroRNAs (miRNAs) are biomolecules composed of nucleotides of 25 to 30 base pairs that are involved in the regulation of these proinflammatory mediators. The objective of this work is to characterize the expression profile of miRNAs 155 and 181 biomolecules in patients with primary and secondary Sjögren Syndrome as autoimmune etiology markers in those with dry eye. Previous studies have made this measurement for miRNA 155 in saliva, plasma and for 181 only in plasma. Therefore, its measurement in tears has not been carried out in any study that has been published. This study was carried out in the ophthalmology service with subsequent patients from the corneal consultation. In total, 40 patients were included, divided into 20 patients with dry eye disease (EOS), including 8 patients with primary and secondary Sjögren's syndrome and 20 healthy patients (CTRL) matched by the same age and sex. Plasma and tear samples were analyzed by PCR from each of the patients, finding a significant overexpression of miR-155 in tears for patients with EOS. For miRNA 181 the same sensitivity was not found, therefore a larger sample size is suggested.


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