2000, Number 4
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Microbiología 2000; 42 (4)
Development and characterization of a mixed nutrient base for the culture of a wide Range of microorganisms
Tsoraeva A, Zhurbenko R
Language: English
References: 24
Page: 155-161
PDF size: 99.69 Kb.
ABSTRACT
Culture media, designed to grow as many different genera of microorganisms as possible, appear to require a nutrient base composed of polypeptides, oligopeptides and amino acids. Most modern culture media are composed by a mixture of different protein hydrolysates obtained from different proteins and enzymes in order to provide the widest spread of peptides. It seems that large peptides have a role in recovering (or resuscitating) of nutritionally fastidious organisms. The aim of this work was to develop a mixed nutrient base with local raw materials and technology. It was made physical, chemical and microbiological characterisation of three mixtures of the protein hydrolysates and it was selected the best one to promote the growth of a wide range of the microorganisms in different culture media.
REFERENCES
Bailey, F. J. and W. K. Herber (inventors). 1994. Merck and Co. Inc., assignee. Method of clonal growth of Streptococcus pneumoniae, US patent 5314822.
Barney, M. C., E. Kot and E. Jand Chicoye (inventors, 1990 March 6). Miller Brewing Company, assignee. Culture medium for detection of beer spoilage microorganisms, US patent 4906573.
Barrow, G. I. and R. K. A. Felthman. 1993. Cowan and Steel¢s manual for identification of medical bacteria, p. 9, 209, 3rd ed. Cambridge University Press, Great Britain.
Bridson, E. Y. 1994. The development, manufacture and control of microbiological culture media, p. 8-39. Unipath Ltd, UK.
Determination of Kjeldahl nitrogen content with Kjeltec System 1026. 1987. Application Note 10, Tecator.
Goyal, A. and S. S. Katiyar. 1997. Effect of certain nutrients on the production of dextransucrase from Leuconostoc mesenteroides NRRL b -512F. J. Basic Microbiol. 37:197-204.
Jenkins, R. O. 1992. The estimation of biomass, p. 53-78. In: Cartledge, T.G. (ed.) In Vitro Cultivation of Micro-organisms Butterworth Heinemann, BIOTOL (Biotecnology by Open Learning), Thomson Litho, East Kilbride, Scotland, Great Britain.
Koneman, E. W., S. D. Allen, W. M. Janda, P. C. Schreckenberger and W. C. Winn. 1997. Introduction to microbiology: Part I. Processing of cultures, p.95-98. In: Allen, A. (ed.) Color Atlas and Textbook of Diagnostic Microbiology, 5th ed. J. B. Lippincott Company, Philadelphia.
Mach, P. A., K. E. Hesselroth, C. A. Adams and D. L. Schwab (inventors, 1998 March 3). Minnesota Mining and Manufacturing Company, assignee. Culture medium for rapid count of coliform bacteria, US patent 5723308.
Mach, P. A. (inventor; 1995 Oct. 31). Minnesota Mining and Manufacturing Company, assignee. Conditioned culture medium for rapid growth and detection of microbes, US patent 5462860.
Manual de Medios de Cultivo. 1994. Bases para medios de cultivo, p. 289. E. Merck, Darmstadt, Alemania.
Manual Difco. 1984. Medios de Cultivo deshidratados y reactivos para microbiología, p. 1031-1037, Décima Edición, Difco, Francisco Soria Melguizo, S.A., Madrid.
Manual SIGMA. 1998. Biochemicals and Reagents for Life Science Research, Microbial Media, p. 1594. Sigma -Aldrich Co. USA.
Meynell, G. G. and E. Meynell. 1965. Theory and Practice in experimental bacteriology, p. 30–60, Ca mbridge University Press, Great Britain.
Morcillo, Z. 1997. Trabajo de Maestría. Estudio del contenido de las sustancias nitrogenadas de origen proteico en las bases nutritivas BioCen. CNB.
Mourey Valdes, L., M. Gurria Rafols, E. Vidrio Sande, J. Bravo Brash and M. D. Bañales. 1990. Manual de normas técnicas sanitarias de agentes de diagnóstico. Secretaria de salud. Subsecretaria de regulación y fomento sanitario. Dirección general de control de insumos para la salud.
NC 57-94. Métodos y medios auxiliares Clínicos. Medios de cultivo. Especificaciones generales de calidad.
Power, D. A. and P. J. McMuen (ed.). 1988. Basic Methods, p. 2-9. In: Manual of BBL. Products and Laboratory Procedures, Sixth Edition. Becton Dickinson Microbiology Systems, USA.
Runova, V. F., L. G. Bendas, G. A. Maksimova, E. I. Preobrazhenskaya, B. M. Raskin and V. A. Melnikova. 1977. Metodicheskie ukazania po primeneniyu fisikokhimicheskikh metodov kontrolia pitatelnikh sred, p. 5-10, Ministerstvo Zdravookhranenia SSSR, Moskwa.
Sonnerwirth, A. C. and L. Jarett. 1983. Gradwolhl. Métodos y diagnósticos del laboratorio clínico. Vol 3, p. 1233-1237, Edición Revolucionaria, Cuba.
Manual Oxoid. 1995. Peptonas, hidrolizados, agares y constituyentes, p. 283-284, Unipath Ltd, España S.A.
Toora, S. (inventor; 1995 Sept. 5). Prince Edward Island Food Tecnology Center, assignee. Growth media and assay for Yersinia enterocolitica, US patent 5447849.
United States Pharmacopoeia XXXIII. Bacteriological tests. 1995. p. 20-25.
Validation Guide for Nutrient Pad Sets. 1992. Sartorius.