medigraphic.com
Revista Latinoamericana de Microbiología

2000, Number 4

<< Back Next >>

Microbiología 2000; 42 (4)

Comparison of two culture media for selective isolation and membrane filter enumeration of Pseudomonas aeruginosa in water

Tsoraeva A, Rodríguez MC

Language: English
References: 18
Page: 149-154
PDF size: 61.31 Kb.


ABSTRACT

Pseudomonas aeruginosa is generally considered to be an ubiquitous bacteria of water and soils, nevertheless in recent years particular attention has been paid to this microorganism because of its increasing significance as a human pathogen and its high resistance to most antibiotics. Nowadays, there are a variety of methods for the detection of P. aeruginosa using different culture media. The aim of this work was to compare the recovery of P. aeruginosa and the selectivity of a synthetic and an undefined culture media (m-Acetamide and m-Cetrimide) by membrane filtration method. Diluted suspensions (10 -6 ) of test bacterial cultures (ATCC collection) and simulated tap water samples (inoculated with highly diluted suspension of P. aeruginosa ATCC 27853) were analysed. The statistical analysis of simulated water samples data showed a significant difference (p ‹ 0.05) in the enumeration of P. aeruginosa from water samples tested in m-Cetrimide and in m-Acetamide media, in favour of the latter. Overmore, m-Acetamide medium showed to be more selective and inhibitory for microorganisms other than P. aeruginosa. Sensiti vity values were 96 and 100%, specificity values 95 and 100% and mean relative recovery frequency values 66.2 and 95.76 % for m-Cetrimide and m-Acetamide media, respectively. On the other hand, the ad-vantage of m-Cetrimide medium was the faster growth of this microorganism.


REFERENCES

  1. Anon. (1994b) The microbiology of water. 1994. Part 1- Drinking Water . Report on Public Health and Medical Subjects, 71, HMSO, London.

  2. Atlas, R. M. 1995. Alphabetical listing of media, p. 12. In Handbook of Microbiological Media for the Examination of Food, Library of Congress Cataloging-in- Publication Data.

  3. Bergey, D. H. 1994. Bergey¢s Manual of Determinative Bacteriology. Ed. by Holt J. G., 9 th ed.

  4. Bernagozzi, M., R. Sacchetti and L. Polenta. 1996. An evaluation of certain Salmonella detection methods in surface water. International J. Hyg. Environ. Medicine 199:84-90.

  5. Byrd, J. J., X. Huai Shu and R. R. Colwell. 1991. Viable but nonculturable bacteria in drinking water. Appl. Environ. Microbiol. 57:875.

  6. Clesceri, L. S., A. E. Greenberg and R. R Trussell. 1989. Microbiological examination, pp. 9-1–9-155. In Standard Methods for examination of water and wastewater. 17th ed. APHA, USA.

  7. Gray, R. D. and G. H. Lowe. 1976. The preparation of simulated water samples for the purpose of bacteriological quality control. J. Hyg. 76:49.

  8. Havelaar, A. H., S. H. Heisterkamp, J. A. Hoekstra and K. A. Mooijman. 1993. Performance characteristics of methods for the bacteriological examination of water. Wat. Sci. Tech. 27:1.

  9. Manaia, C. M., O. C. Nuñes, P. V. Morais and M. S. Da Costa. 1990. Heterotrophic plate counts and the is olation of bacteria from mineral waters on selective and enrichment media. J. Appl. Bacteriol. 69:871.

  10. Nicoletti, G. and V. M. Nicolosi. 1990. Diccionario de Bacteriología Humana, Centro Documentación Científica Menarini, 170-182.

  11. Norma Cubana 76-02. 1982. Preparación de medios de cultivo y reactivos. Comité Estatal de Normalización, Cuba.

  12. O¢ Leary, W. M. 1990. Practical Handbook of Microbiology, Library of Congress Cataloging-in-Publication Data.

  13. Parámetros Microbiológicos. 1997. p. 42, 54-57. In: Panreac Química, S.A. (ed.) Aguas potables de consumo público y aguas de bebida envasadas.

  14. Place, B. M., H. E. Tillet and N. F. Lightfoot. 1996. Note: Development of an external quality assurance scheme for the detection and enumeration of Pseudomonas aeruginosa from water and comparison of results using modified King’s A broth and a commercial agar. J. Appl. Bacteriol. 80:682.

  15. Power, D. A. and P. J. McMuen (ed.). 1988. Basic Methods, p. 2-9. In Manual of BBL. Products and Laboratory Procedures, Sixth Edition. Becton Dickinson Microbiology Systems, USA.

  16. Szita, G and G. A. Biro. 1990. Synthetic, selective culture medium for Pseudomonas aeruginosa. Acta Veterinaria Hungarica 38 (3): 187.

  17. The United States Pharmacopoeia XXIII. 1995.United States Pharmacopeial Convention, Rockville, Md., USA.

  18. Validation Guide for Nutrient Pad Sets. 1992. Sartorius, Germany.




2020     |     www.medigraphic.com