2021, Number 2
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Rev Cubana Plant Med 2021; 26 (2)
Leaf morphology of Raulinoreitzia crenulata and antioxidant activity of its essential oils
Pereira MFAC, Batista TM, Loureiro SFA, de Souza LF, de Souza CCF
Language: Spanish
References: 18
Page:
PDF size: 406.68 Kb.
ABSTRACT
Introduction: The species Raulinoreitzia crenulata grows in open forest areas of south, southeast, northeast and center-west Brazil and Bolivia.
Objective: Evaluate the leaf morphology of R. crenulata and the antioxidant activity of essential oils from its leaves, branches and flowers.
Methods: Diaphanization was performed with two dyes, safranin and toluidine blue for venation patterns. The leaf, branch and flower oils were extracted in a Clevenger set and their yield was determined. Antioxidant activity was identified with the free radical DPPH at various concentrations: 50-1.0 mg ml-1.
Results: R. crenulata leaves are opposite, with the first vein acrodrome, anomocytic stomata on both sides, more abundant on the abaxial side, and presence of glandular trichomes and tectors. Their venation pattern covers up to the fourth order, FEVs with three branches, full areolae with four or five sides, without the presence of marginal nerves. Essential oil output was 0.533%, 0.437% and 0.461% for leaves, branches and flowers, respectively. Antioxidant activity displayed a 100% reduction at concentrations of 20-50 mg ml-1 for branch and flower oils and 10 mg ml-1 for leaf oils.
Conclusions: The results obtained from the leaf morphology analysis of Raulinoreitzia crenulata will be included in studies aimed at characterizing this species as well as the remaining taxa of the genus Eupatorium. Essential oil content displayed high potential, placing R. crenulata among the possible sources of essential oils with important biological activity in the reduction of DPPH free radicals.
REFERENCES
Panero JL, Funk VA. Toward a phylogenetic classification for the Compositae (Asteraceae). Proc Biol Soc. 2002 [acceso: 10/12/2021];115:909-22. Disponible en: https://repository.si.edu/bitstream/handle/10088/11384/bot_2002_pr_Panero_Funk_SubfamilialCompositae.pdf
Silva TDG, Marzinek J, Hattori EKO, Nakajima JN, De-Paula OC. Comparative cypsela morphology in Disynaphiinae and implication for their systematics and evolution (Eupatoriaea: Asteraceae). Bot J Linn Society. 2018;186(1):89-107. DOI: https://doi.org/10.1093/botlinnean/box082
Hattori EKO. Raulinoreitzia in Flora do Brasil 2020 em construção. Jard Bot Rio Jan. 2019 [acceso: 10/12/2021]. Disponible en: http://www.floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB16281
Liu PY, Liu D, Li WH, Zhao T, Sauriol F, Gu YC, et al. Chemical constituints of plants from the genus Eupatorium (1904-2014). Chem Biodivers. 2015;12(10):1481-515. DOI: https://doi.org/10.1002/cbdv.201400227
Sobrinho ACN, De Morais SM, De Souza EB, Fontenelle Rodos S. The genus Eupatorium L. (Asteraceae): A review of their Antimicrobial activity. J Medic Pl Res. 2017;11(3):43-57. DOI: https://doi.org/10.5897/JMPR2016.6313
Sobrinho ACN, De Souza EB, Rocha MFG, Albuquerque MRJ, Bandera PN, Dos Santos HS, et al. Cytotoxicity, antifungal and antioxidant activities of the essential oil from Eupatorium ballotifolium Kunth (Asteraceae). A J Phar Pharmco. 2016;10(16):346-55. DOI: https://doi.org/10.5897/AJPP2016.4537
Souza JT, Apel MA, Bordignon S, Matzenbacher NI, Zuanazzi JÂS, Henriques AT. Composição química e atividade antioxidante do óleo volátil de Eupatorium polystachyum DC. Rev Bras Farmac. 2007;17(3):368-72. DOI: https://doi.org/10.1590/S0102-695X2007000300011
Garcia E, Lopez BR, Torres NR, Pacheco MM. A revision de Eupatorium (Compositae: Eupatorieae) from Michiacan. Phyton. 2011 [acceso: 10/12/2021];80:139-46. Disponible en: https://www.proquest.com/scholarly-journals/revision-i-eupatorium-compositae-eupatorieae/docview/2398006854/se-2
Fonsêca CM, Proença EB, Gonçalves EG. Descrição do padrão de venação foliar em Spathicarpa Hook. (Araceae). Acta Bot Bras. 2006;21(1):213-21. DOI: https://doi.org/10.1590/S0102-33062007000100020 10. Dos Reis RE, Alvim MN. Anatomia foliar comparada de três espécies do gênero Oxalis L. (Oxalidaceae). NBC. 2013;3(6):59-72. DOI: http://dx.doi.org/10.15601/2238-1945/pcnb.v3n6p59-72
Hickey LJ. Classification of arquitecture of dicotyledonous leaves. Am J Bot. 1973;0(1):17-33. DOI: https://doi.org/10.1002/j.1537-2197.1973.tb10192.x
Mezza NG, Borgarello AV, Grosso NR, Fernandez H, Pramparo MC, Gayol MF. Antioxidant activity of rosemary essential oil fractions obtained by molecular distillation and their effect on oxidative stability of sunflower oil. Food Chemistry. 2018;242:9-15. DOI: https://doi.org/10.1016/j.foodchem.2017.09.042
Sundararajan B, Moola AK, Vivek K, Kumari BDR. Formulation of nanoemulsion from leaves essential oil of Ocimum basilicum L. and its antibacterial, antioxidant and larvicidal activities (Culex quinquefasciatus). Micro Pathog. 2018;125:475-85. DOI: https://doi.org/10.1016/j.micpath.2018.10.017
Sangwan NS, Farooqi AHA, Shabih F, Sangwan RS. Regulation of essential oil production in plant. Plant Growth Regul. 2001;34:3-21. DOI: https://doi.org/10.1023/A:1013386921596
Sacchetti G, Maiett S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, et al. Comparative evaluation of 11 essential oils of different origin as funtional antioxidants, antiradicals and antimicrobials in foods. Food Chem. 2005;91(4):621-32. DOI: https://doi.org/10.1016/j.foodchem.2004.06.031
Ash A, Ellis B, Hickey L, Johnson K. Manual of leaf arquitecture. Morphological description and categorization of dicotyledonous and net-veined monocotyledonous angiosperms. Smithson Inst. 1999:67. DOI: http://dx.doi.org/10.13140/2.1.3674.5282
Maleski LT, Souza MT, Aguiar LK. Toxicidade do óleo essencial de Raulinoreitzia crenulata (Asteraceae) e seu constituinte majoritário contra Aphis forbesi (Hemiptera: Aphididae). Anais Cong Bras Fitossan. 2019 [acceso: 10/12/2021]. Disponible en: http://fitossanidade.fcav.unesp.br/seer/index.php/anaisconbraf/article/view/486/386
Pereira FC, Souza LF, Guilherme FAG, Freire JC, Teles AM. Diversidade de Asteraceae em um campo de murundus no sudoeste de Goiás, Brasil. Rodriguésia. 2019;70. DOI: https://doi.org/10.1590/2175-7860201970015
Scremin Dias E, Potti VJ, Damasceno GA, Cristaldo ACM, Dias FA, Pott A. Flora de campos úmidos calcários da Serra da Bodoquena. Iheringia. 2018;73:80-92. DOI: https://doi.org/10.21826/2446-8231201873s80