del Barrio AGC, Jardines FY, Spengler SI, García PTH, Roque QA, Alvarez VM

Language: Spanish
References: 23
Page: 1-15
PDF size: 364.56 Kb.

ABSTRACT

Introduction: Dengue is the fastest spreading mosquito-borne viral disease worldwide. Despite its high incidence, no effective antiviral treatment or vaccine has been approved for use against this infection. The plant Ageratina havanensis has been widely used in our folk culture and in other countries due to its various biological properties.
Objective: Evaluate the antiviral activity of four extracts from Ageratina havanensis and two flavonoids isolated from that plant against the dengue 2 virus.
Methods: Primary screening of the antiviral activity of each extract and flavonoid was conducted by viral productivity detection assay, and an evaluation was carried out of the virucidal action of the stem ethanolic extract and the leaf butanolic extract.
Results: All the extracts inhibited viral replication by a reduction in the number of lysis plates. However, no evidence was found of a reduced viral infective titer in the virucidal assay. Treatment with sakuranetin achieved viral productivity inhibition values of 61%-91%, whereas 7-methoxyaromadendrin obtained viral reduction values of 64%-86%.
Conclusions: The extracts and flavonoids isolated from Ageratina havanensis displayed antiviral activity. The stem ethanolic extract and the leaf butanolic extract do not have a virucidal action against the viral strain used. The paper is the first report about the effect of extracts and compounds isolated from A. havanensis against the dengue virus.

REFERENCES

1. Stuart TP, Buck MD, Shresta S. Better late than never: antivirals for dengue. Expert Rev Anti Infect Ther. 2011;9(7):755-757.

2. 2 . Gubler DJ, Kuno G, Markoff L. Flaviviruses. In: Knipe DM, Holey PM, editors. Fields of Virology, 5th ed. Wolters Kluwer and Lippincott Williams & Wilkins; 2007. p. 1153-252.

3. 3 . Costa da Silveira Oliveira AF, Teixeira R, Silva de Oliveira A, Martins de Souza AP, Lopes da Silva M, Oliveira de Paula S. Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses. Molecules. 2017;22:505.

4. 4 . WHO. Dengue: guidelines for diagnosis, treatment, prevention, and control Spec Program Res. Train Trop Dis. 2009;47.

5. Teixeira RR, Pereira WL, Oliveira AF, da Silva AM, de Oliveira AS, da Silva ML, et al.. Natural Products as Source of Potential Dengue Antivirals. Molecules. 2014;9:8151-76.

6. del Barrio G, Spengler I, García T, Roque A, Álvarez AL, Calderón JS, et al. Antiviral activity of Ageratina havanensis and major chemical compounds from the most active fraction. Brazilian Journal of Pharmacognosy. 2011;21:915-20.

7. 7 . Garcia TH, Quintino da Rocha C, Dias M, Pino ll, del Barrio G, Roque A, et al. Comparison of the qualitative chemical composition o extracts from Ageratina havanenesis collected in two different phenological stages by FIA-ESI-IT-MSn and UPLC/ESI-MSn Antiviral activity. Natural Product Communications. 2017;1(12):1-152.

8. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth.1983;(65):55-63.

9. 9 . Alvarez M, Rodriguez-Roche BL, Morier L, Guzmán MG. Improved Dengue Virus Plaque Formation on BHK21 and LLCMK2 Cells: Evaluation of Some Factors. Bulletin Dengue. 2005;29.

10. 10 . Gubler JD. Dengue/dengue haemorrhagic fever: history and current status. In: Bock G, Goode J (eds). New treatment strategies for dengue and other flaviviral diseases. Chichester: Novartis Foundation; 2006.

11. Gubler DJ, Kuno G, Markoff L. Flaviviruses. In: Knipe DM, Holey PM, editors. Fields of Virology. 5th ed. Wolters Kluwer and Lippincott Williams & Wilkins; 2007. p. 1153-252.

12. Abd Kadir SL. Potential anti-dengue medicinal plants: a review. J Nat Med. 2013;67(4):677-89.

13. Singh PK, Rawat P. Evolving herbal formulations in management of dengue fever. Journal of Ayurveda and Integrative Medicine. 2017;8:207-10.

14. Kaushik S, Kaushik S, Sharma V, Yadav JP. Antiviral and therapeutic uses of medicinal plants and their derivatives against dengue viruses. Phcog Rev. 2018;12:177-85.

15. Ali-Seyed M, Vijayaraghavan K. Dengue virus infections and anti-dengue virus activities of Andrographis paniculata. Asian Pac J Trop Med. 2020;13(2):49-55.

16. Talarico LB, Pujol CA, Zibetti RGM, Faría PCS, Noseda MD, Duarte MER, et al. The antiviral activity of sulfated polysaccharides against dengue virus is dependent on virus serotype and host cell. Antiviral Research. 2005;66(2-3):103-10.

17. Talarico LB, Damonte EB. Interference in dengue virus adsorption and uncoating by carrageenans. Virol. 2007;363:473-85.

18. Talarico LB, Noseda MD, Ducatti DRB, Duarte MER, Damonte EB. Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan. J Gen Virol. 2011;92 (6):1332-42.

19. Lyus RJ, Park W. Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro. Arch Pharm Res. 2005;28:1293-1301.

20. Fernández-Romero J, Romeu B, Gutiérrez Y, Valdés VS. Parra F. In vitro antiviral activity of Phyllanthus orbicularis extracts against herpes simplex virus type 1. Phytother Res. 2003;17:980-2.

21. Silva IT, Costa GM, Stoco PH, Schenkel EP, Reginatto FH, Simões CM. In vitro antiherpes effects of a C-glycosylflavonoid-enriched fraction of Cecropia glazioviisneth. Lett Appl Microbiol. 2010;51:143-8.

22. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, and AbuBakar S. In vitro antiviral activity of fisetin, rutin and naringenin against dengue virus type-2. J Med Plants Res. 2011;5(23):5534-9.

23. Silva ARA, Morais SM, Marques MMM, Lima DM, Santos SCC, Almeida RR, Vieira IGP, Guedes MIF. Antiviral activities of extracts and phenolic components of two Spondias species against dengue virus. JVATiTD. 2011;17:406-13.