Cerdá R, Xavier J, Sansalone P, de la Sota R, Rosenbush R

Language: Spanish
References: 22
Page: 7-12
PDF size: 59.86 Kb.

ABSTRACT

Several mycoplasma species produce various diseases in different animal species. M. bovis has been described as the cause of mastitis, arthritis, pneumonia and infertility in cattle. Furthermore, this specie has been the most frequently isolated agent producing bovine mastitis. The objective of this study was to isolate and typify mycoplasma strains from a clinical mastitis outbreak in a dairy farm of Buenos Aires Province. A total of 279 samples were studied (276 from pooled quarter milk of cows with clinical mastitis that did not respond to antibiotic therapy, 1 from bulk tank milk and 2 prepucial swabs from bulls). The isolated mycoplasma strains (n=12) were further characterized by biochemical analysis, serological studies and electrophoretic analysis of the protein profiles (SDS-PAGE). Based upon these studies, the isolated strains were identified as Mycoplasma bovis. This is the first report of isolation of this microorganism in Argentina. Therefore the results described here could be very useful to improve mastitis control in dairy farms.

REFERENCES

1. Barile, M. F. 1983. Arginine hydrolysis, p. 345-349. In: S. Razin and J.G. Tully (ed.). Methods in Mycoplasmology, Vol. 1. Academic Press, Inc. New York.

2. Bayoumi, F. A., T. B. Farver; B. Bushnell y M. Oliveira. 1988. Enzootic mycoplasmal mastitis in a large dairy during an eigth-year period. J. Am. Vet. Med. Assoc. 192:905-909.

3. Boughton, E. 1978. Mycoplasma bovis mastitis. Vet. Rec. 103:2270-2271.

4. Freundt, E. A. 1983. Culture media for classic mycoplasma, p. 127- 135. In: S. Razin y J. G. Tully (ed.). Methods in Mycoplasmology, Vol. 1. Academic Press Inc. New York.

5. Freundt, E. A. 1983. Film and spot production. En: S. Razin and J. G. Tully (ed.). Methods in Mycoplasmo logy, p. 373-378. Vol. 1. Academic Press, Inc. New York.

6. Gardella, R. S., R. A. Delgiudice y J. G. Tully. 1983. Inmunofluorescence. En: S. Razin and Tully J.G. (ed.). Methods in Mycoplasmology, p. 431- 439 Vol. 1. Academic Press Inc. New York.

7. Gonzalez, R. M., P. M. Sears y R. A. Merrill. 1992. Mastitis due to mycoplasma in the state of New York during the period of 1972 -1990. Cornell Vet. 82:29.

8. Gourlay, R. N. y C. J. Howard. 1979. Bovine mycoplasmas. En: M. F. Barile; S. Razin; J. G. Tully and R. F. Whitcomb (ed.). p.49-102 The Mycoplasmas, Vol 2. Academic Press, New York.

9. Hernandez Andrade, L. y L. Jaramillo Meza. 1995. Comparación de pruebas serológicas para el diagnóstico de mastitis por Mycoplasma bovis. Vet. Mex. 26:353-357.

10. Hotzel, H., K. Sachse, H. Pfutzner, B. Demuth y A. Pflitsch. 1993. Detection of Mycoplasma bovis using in vitro deoxyribonucleic acid amplification. Rev. Sci. Tech. Off . Int . Epiz. 12:581-591.

11. Jasper, D. E. 1979. Bovine mycoplasmal mastitis. J. Am. Vet. Med. Assoc. 175:1072.

12. Jasper, D. E. 1982. The role of Mycoplasma in bovine mastitis. J. Am. Vet. Med. Assoc. 181:158.

13. Jasper, D. E. 1981. Bovine mycoplasmal mastitis. Adv. Vet. Sci. Comp. Med. 25:121-157.

14. Jasper, D. E. 1977. Mycoplasma and Mycoplasma Mastitis. JAVMA, Vol. 170, Nº 10 (2).

15. Jasper, D. E., J. M. Al-Aubaidi, y J. Fabricant. 1974. Isolation of Mycoplasma from preputial washings of bulls. Cornell Vet. 64: 296.

16. Jasper, D. E., J. M. Al-Aubaidi, y J. Fabricant. 1974. Epidemiological observations on mycoplasma mastitis. Cornell Vet. 64:407.

17. Kissi, B., S. Juhosz, y L. Stipkovits. 1985. Effect of mycoplasma contamination of bull semen on fertilization. Acta. Vet. Hung. 33:107.

18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assemly of the head of bacteriophage T4. Nature 227:6680-685.

19. Pfutzner H. 1984. Die Mycoplasma bovis infektion des rindes. Mh. Vet. Med. 39: 217-220.

20. Razin, S. y V. P. Cirillo. 1983. Sugar fermentation. En: S. Razin and J.G. Tully (ed.). Methods in Mycoplasmology. p. 337- 343 Vol. 1. Academic Pres. Inc. New York.

21. Senterfit, L. 1983. Tetrazolium reduction inhibition, p. 419- 421. En: S. Razin and J.G. Tully (ed.). Methods in Mycoplasmology, Vol. 1. Academic Press, Inc. New York.

22. Tully, J. G. 1983. Test for digitonin sensitivity and sterol requirement. En: S. Razin and J.G. Tully (ed.). Methods in Mycoplasmology, p. 355- 362 Vol. 1. Academic Pres. Inc. New York.