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ISSN 1659-1046 (Print)
Odovtos - International Journal of Dental Sciences

2022, Number 1

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Odovtos-Int J Dent Sc 2022; 24 (1)

Physicochemical Properties and Antibiofilm Activity of Tricalcium Silicate Cement and its Association with Cetrimide

Bueno RG, Tanomaru-Filho M, Chavez-Andrade GM, Esteves TFF, Guerreiro-Tanomaru JM

Language: English
References: 32
Page: 113-121
PDF size: 208.87 Kb.


ABSTRACT

Cetrimide (CTR) is a cationic surfactant detergent with antimicrobial and antibiofilm activity. The aim of this study was to evaluate setting time, pH, solubility and antibiofilm activity of tricalcium silicate cement (TSC) with zirconium oxide (ZrO2) and its association with 0.2 and 0.4% cetrimide. Initial and final setting times (IST and FST) were assessed based on ISO-6876. pH was evaluated at periods of 1,3,7,14 and 21 days. Solubility was analyzed by weight loss. A modified direct contact test (MDCT) on the biofilm of Enterococcus faecalis formed on bovine root dentin blocks was performed, after 6 hours of manipulation and 15 hours of contact time. The analysis was performed by UFC mL ¯¹ counting. The data were analyzed by ANOVA and Tukey’s tests (α=0.05). Higher IST was observed for TSC/ZrO2+CTR in both concentrations than for TSC/ZrO2 and lower FST for TSC/ZrO2+0.4% CTR (p‹0.05). On day 1, TSC/ZrO2 showed lower pH than the associations with CTR (p‹0.05). During the other periods, TSC/ZrO2 and associations promoted similar alkalinization (p›0.05). All materials exhibited increased mass. TSC/ZrO2+CTR 0.4% had lower mass gain than the other materials (p‹0.05). The highest antibiofilm activity was observed for TSC/ZrO2+CTR in both concentrations, when compared with the positive control (p‹0.05). In conclusion, CTR exhibited potential to promote greater antibiofilm activity to tricalcium silicate cement, without harming its physicochemical properties of setting time, pH and solubility.


REFERENCES

  1. Brenes-Valverde K., Conejo-Rodríguez E., Vega-Baudrit J.R., Montero-Aguilar M., Chavarría-Bolaños D. Evaluation of Microleakage by Gas Permeability and Marginal Adaptation of MTA and Biodentine™ Apical Plugs. ODOVTOS - Int. J. Dental Sc. 2018; 20 (1): 57-67.

  2. Lucas C.P., Viapiana R., Bosso-Martelo R., Guerreiro-Tanomaru J.M., Camilleri J., Tanomaru-Filho M. Physicochemical Properties and Dentin Bond Strength of a Tricalcium Silicate-Based Retrograde Material. Braz Dent J. 2017; 28 (1): 51-56.

  3. Marciano M.A., Guimaraes B.M., Amoroso- Silva P., Camilleri J., Hungaro Duarte M.A. Physical and Chemical Properties and Subcutaneous Implantation of Mineral Trioxide Aggregate Mixed with Propylene Glycol. J Endod. 2016; 42 (3): 474-479.

  4. Siboni F., Taddei P., Prati C., Gandolfi M.G. Properties of NeoMTA Plus and MTA Plus cements for endodontics. Int Endod J. 2017; 50 Suppl 2 (Suppl 2): e83-e94.

  5. Cordeiro M.M., Santos A.S., Reyes Carmona J.F. Mineral Trioxide Aggregate and Calcium Hydroxide Promotes In Vivo Intratubular Mineralization. ODOVTOS - Int. J. Dental Sc. 2016; 18 (1): 49-59.

  6. Kahler B., Chugal N., Lin L.M. Alkaline Materials and Regenerative Endodontics: A Review. Materials (Basel). 2017; 10 (12).

  7. Torres F.F.E., Perinoto P., Bosso-Martelo R., Chávez-Andrade G.M., Guerreiro-Tanomaru J.M., Tanomaru-Filho M. Influence of Powder-to-Gel Ratio on Physicochemical Properties of a Calcium Silicate Sealer. ODOVTOS - Int. J. Dent. Sc. 2020; 22 (3): 154-162.

  8. Cavenago B.C., Del Carpio-Perochena A.E., Ordinola-Zapata R., Estrela C., Garlet G.P., Tanomaru-Filho M., Weckwerth P.H., de Andrade F.B., Duarte M.A.H. Effect of Using Different Vehicles on the Physicochemical, Antimicrobial, and Biological Properties of White Mineral Trioxide Aggregate. J Endod. 2017; 43 (5): 779-786.

  9. Vazquez-Garcia F., Tanomaru-Filho M., Chavez-Andrade G.M., Bosso-Martelo R., Basso-Bernardi M.I., Guerreiro-Tanomaru J.M. Effect of Silver Nanoparticles on Physicochemical and Antibacterial Properties of Calcium Silicate Cements. Braz Dent J. 2016; 27 (5): 508-514.

  10. Xuereb M., Sorrentino F., Damidot D., Camilleri J. Development of novel tricalcium silicate-based endodontic cements with sintered radiopacifier phase. Clin Oral Investig. 2015; 20 (5): 967-982.

  11. Li X., Yoshihara K., De Munck J., Cokic S., Pongprueksa P., Putzeys E., Pedano M., Chen Z., Van Landuyt K., Van Meerbeek B. Modified tricalcium silicate cement formulations with added zirconium oxide. Clin Oral Investig. 2017; 21 (3): 895-905.

  12. Camilleri J. Is Mineral Trioxide Aggregate a Bioceramic? ODOVTOS - Int. J. Dent. Sc. 2016; 18 (1): 13-17.

  13. Antonijevic D., Medigovic I., Zrilic M., Jokic B., Vukovic Z., Todorovic L. The influence of different radiopacifying agents on the radiopacity, compressive strength, setting time, and porosity of Portland cement. Clin Oral Investig. 2014; 18 (6): 1597-1604.

  14. Tanomaru M., Viapiana R., Guerreiro J. From MTA to New Biomaterials Based on Calcium Silicate. ODOVTOS - Int. J. Dental Sc. 2016; 18 (1): 18-22.

  15. Camilleri J., Sorrentino F., Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater. 2013; 29 (5): 580-593.

  16. Widbiller M., Lindner S.R., Buchalla W., Eidt A., Hiller K.A., Schmalz G., Galler K.M. Three-dimensional culture of dental pulp stem cells in direct contact to tricalcium silicate cements. Clin Oral Investig. 2016; 20 (2): 237-246.

  17. Khalil I., Naaman A., Camilleri J. Properties of Tricalcium Silicate Sealers. J Endod. 2016; 42 (10): 1529-1535.

  18. Setbon H.M., Devaux J., Iserentant A., Leloup G., Leprince J.G. Influence of composition on setting kinetics of new injectable and/or fast setting tricalcium silicate cements. Dent Mater. 2014; 30 (12): 1291-1303.

  19. International Organization for Standardization Dentistry. Root canal sealing materials. British Standards Institution ISO 6876. London, UK. 2012.

  20. Silva G.F., Bosso R., Ferino R.V., Tanomaru- Filho M., Bernardi M.I., Guerreiro- Tanomaru J.M., Cerri P.S. Microparticulated and nanoparticulated zirconium oxide added to calcium silicate cement: Evaluation of physicochemical and biological properties. J Biomed Mater Res A. 2014; 102 (12): 4336-4345.

  21. Valverde M.E., Baca P., Ceballos L., Fuentes M.V., Ruiz-Linares M., Ferrer-Luque C.M. Antibacterial efficacy of several intracanal medicaments for endodontic therapy. Dent Mater J. 2017; 36 (3): 319-324.

  22. Giardino L., Bidossi A., Del Fabbro M., Savadori P., Maddalone M., Ferrari L., Ballal N.V., Das S., Rao B.S.S. Antimicrobial activity, toxicity and accumulated hard-tissue debris (AHTD) removal efficacy of several chelating agents. Int Endod J. 2020; 53 (8): 1093-1110.

  23. Ruiz-Linares M., Aguado-Perez B., Baca P., Arias-Moliz M.T., Ferrer-Luque C.M. Efficacy of antimicrobial solutions against polymicrobial root canal biofilm. Int Endod J. 2017; 50 (1): 77-83.

  24. Qaiser S., Hegde M.N., Devadiga D., Yelapure M. Root dentin surface activation to improve bioceramic bonding: A scanning electron microscopic study. J Dent Res Dent Clin Dent Prospects. 2020; 14 (2): 117-123.

  25. Gjorgievska E., Apostolska S., Dimkov A., Nicholson J.W., Kaftandzieva A. Incorporation of antimicrobial agents can be used to enhance the antibacterial effect of endodontic sealers. Dent Mater. 2013; 29 (3): e29-34.

  26. Bailon-Sanchez M.E., Baca P., Ruiz-Linares M., Ferrer-Luque C.M. Antibacterial and anti-biofilm activity of AH plus with chlorhexidine and cetrimide. J Endod. 2014; 40 (7): 977-981.

  27. Ruiz-Linares M., Bailon-Sanchez M.E., Baca P., Valderrama M., Ferrer-Luque C.M. Physical properties of AH Plus with chlorhexidine and cetrimide. J Endod. 2013; 39 (12): 1611-1614.

  28. Singh S., Podar R., Dadu S., Kulkarni G., Purba R. Solubility of a new calcium silicatebased root-end filling material. J Conserv Dent. 2015; 18 (2): 149-153.

  29. Alsubait S., Albader S., Alajlan N., Alkhunaini N., Niazy A., Almahdy A. Comparison of the antibacterial activity of calcium silicateand epoxy resin-based endodontic sealers against Enterococcus faecalis biofilms: a confocal laser-scanning microscopy analysis. Odontology. 2019; 107 (4): 513-520.

  30. Costa B.C., Guerreiro-Tanomaru J.M., Bosso-Martelo R., Rodrigues E.M., Bonetti- Filho I., Tanomaru-Filho M. Ytterbium Oxide as Radiopacifier of Calcium Silicate- Based Cements. Physicochemical and Biological Properties. Braz Dent J. 2018; 29 (5): 452-458.

  31. Ruiz-Linares M., Ferrer-Luque C.M., Arias-Moliz T., de Castro P., Aguado B., Baca P. Antimicrobial activity of alexidine, chlorhexidine and cetrimide against Streptococcus mutans biofilm. Ann Clin Microbiol Antimicrob. 2014; 13 41.

  32. Simoes M., Pereira M.O., Vieira M.J. Effect of mechanical stress on biofilms challenged by different chemicals. Water Res. 2005; 39 (20): 5142-5152.




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