de la Torre-González C, Hernández-Alcántara LE, Gómez-Ramírez U, Velázquez-Guadarrama N

Language: Spanish
References: 29
Page: 1-15
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ABSTRACT

Objective: To evaluate the changes in the microbiota in the chronic cholesteatomatous otitis media after the application of sodium 2-mercaptoethanesulfonate (MESNA) in the pediatric population, through 16S bacterial ribosomal RNA sequencing.
Materials and Methods: A subexperimental, prolective study done at the Otorhinolaryngology service of Hospital Infantil de México Federico Gómez, Mexico City, from December 2017 to December 2018, including children with chronic cholesteatomatous otitis media who underwent primary or revision mastoidectomy. The RNA of cholesteatomas was obtained (before and after MESNA application). Relative abundance, richness and diversity were determined at the level of phylum and genera.
Results: Twenty-six samples were included, corresponding to 13 children of both sexes, without comorbidities. It was possible to identify more than 98% of the sequences at the level of phylum, class, order, and family. The microbiota was represented by the predominant phyla Firmicutes, Proteobacteria, Actinobacteria, Cyanobacteria and Bacteroidetes. At the genus level, Turicella and Alloiococcus were observed in association with the disease. Likewise, it was observed that after the application of MESNA, the genera Propionibacteria and Staphylococcus associated with the usual microbiota of the middle ear prevailed and increased in abundance. Similarly, an increase in the diversity of bacteria was observed, as well as an increase and emergence of other genera that are part of the endogenous microbiota of the middle ear.
Conclusions: The emergence and prevalence of genera present in the normal healthy middle ear microbiota and the loss of pathogenic genus demonstrate the usefulness of MESNA.

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