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2019, Number 3

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Biotecnol Apl 2019; 36 (3)

Comparison of NucliSens® EasyQ® HIV-1 v2.0 and COBAS® AmpliPrep/COBAS® TaqMan® 48 HIV-1 v2.0 commercial systems for the determination of viral load of different HIV-1 genetic variants in Cuban patients

Pérez L, Vélez S, Díaz HM, Pintos Y, Machado LY, Kourí V, Blanco-de Armas M

Language: English
References: 41
Page: 3221-3226
PDF size: 306.26 Kb.


ABSTRACT

The quantification of viral load of the human immunodeficiency virus type 1 in plasma has become an essential tool to the monitoring of infected patients. The aim of this study was to compare the viral load assays, NucliSENS® EasyQ® HIV-1 v2.0 and COBAS® AmpliPrep/COBAS® TaqMan® 48 HIV-1, in different HIV-1 genetic forms. We collected 109 plasmas from HIV-1 infected patients from Hermanos Ameijeiras Hospital (September 2015 to April 2016). The viral load was quantified using both methods. The viruses were subtyped by in-house sequence-PCR. The medians of viral loads from 54 samples quantified by both methods were different (p < 0.0001). The 52 % of the samples had differences higher than 0.5 log IU/mL. Lin’s coefficient was 0.86 and Bland Altman’s method showed poor concordance between both methods. Viral load levels were different for BGs recombinants between the methods assayed (p < 0.0091), being lower with the EasyQ® system. The EasyQ® method sub-quantifies the Cuban CRF_BGs recombinations, which limits the use of this assay for the clinical monitoring of patients infected with this viral variant. We consider that the monitoring of HIV-1 viral load should be performed only by one method for each patient.


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