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ISSN 1027-2852 (Electronic)
ISSN 0864-4551 (Print)

2019, Number 3

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Biotecnol Apl 2019; 36 (3)

Analysis of genetic polymorphisms among glyphosate-resistant soybean [Glycine max (L.) Merr.] lines

Delgado C, Corrales O, Hernández Y, Soto N, Ferreira A, Jiménez OR, Enríquez GA

Language: English
References: 38
Page: 3301-3306
PDF size: 717.06 Kb.


ABSTRACT

The Cuban National Program for Soybean Genetic Breeding [Glycine max (L.) Merr.] has been focused on improving yield as well as adding new traits for better crop management, including the introgression of transformation event GTS 40-3-2 into advanced lines for conferring resistance to glyphosate herbicide. However, there are no reports on the use of molecular markers to evaluate genotypes obtained from this program. Therefore, in this work, a practical application of RAPD, ISSR and SSR markers is provided, to analyze the genetic diversity, estimate pedigree relationships and confirm genetic identity of nine elite soybean genotypes engaged in the current breeding program. Five glyphosate-resistant soybean lines and their respective parents were studied. Molecular markers were able to distinguish among cultivars. The UPGMA dendrogram, constructed using the three marker systems together, effectively clustered each inbred line with one of their parents. The low genetic similarity coefficient (0.4) obtained from parents IncaSoy1, IncaSoy36, CEB2 and CEB4 confirmed the observed genetic differences. Two genotypes, CEB4 and RP5 were precisely detected from a collection of 15 soybean cultivars during validation testing. RAPD amplicons were converted into two new SCAR markers that successfully detected CEB4 and contributed to build a molecular profile for the promising line IncaSoy36, something crucial for future actions based on the breeding potential of both lines. This study demonstrates that the use of this marker system could provide substantial benefits for soybean breeders and the seed industry in Cuba and other countries which breeding projects rely on local germplasm and facilities.


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