Pérez-León NJ, Castro-Menduiña RI, Echevarría-Hernández A

Language: English
References: 15
Page: 2231-2234
PDF size: 346.33 Kb.

ABSTRACT

The study was aimed to determine the radiosensitivity of three Chickpea varieties (DN-12, DI-18 and DI- 21). Ten different radiation doses were tested on seeds: 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy, in a source of 60Co gamma rays. Once irradiated, 100 seeds per dose and a non-irradiated control were pre-germinated to evaluate the effect seven days post-germination. Stem height and root length of seedlings after 15 days and their survival were assessed after 30 days. Germination percentage and mean lethal dose (LD50) were determined for each cultivar, and medium growth (GR50) and dose-effect curves established for the evaluated characters and cultivars. Optimal irradiation doses for the breeding process were chosen. Afterwards, 1000 irradiated seeds per treatment were selected and donor cultivars were seeded in the field and harvested without making selection and planted again. The respective healthy plants were evaluated for the total number of branches, pods and grains, number of pods with two grains and total mass of grains. The irradiation dosage tested had no effect on germination, while seedling and roots height significantly decreased with dose increase, and a drastic reduction was seen in survival for cultivars DI-18 and DI-21 with 400 Gy and for DN-12 with 500 Gy. Then, 200, 300 and 400 Gy doses were selected to irradiate seeds from the three cultivars and they were evaluated in field planting experiments. Field evaluation provided variability, and six lines were selected due to superior performance and the lack of pest damage.

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