Guerra-Peña Y, Remedios-Jiménez L, Izquierdo-Fiallo K, Gonzales-Aznar E, Fajardo-Sánchez A, Fernández-Castillo S, García-Rivera D, Pérez-Quiñoy JL

Language: Spanish
References: 16
Page: 33-38
PDF size: 390.02 Kb.

ABSTRACT

Typhoid fever caused by Salmonella Paratyphi A (paratyphoid fever) is indistinguishable from that caused by Salmonella Typhi and the degree of incidence has increased in recent years, especially in Southeast Asia. On the other hand, diarrhea and other enteric complications caused by Salmonella Enteritidis and Salmonella Typhimurium continue to be a serious health problem, especially in underdeveloped countries. Vaccines continue to be the most effective way to prevent these diseases. There are vaccines based on Salmonella Typhi capsular polysaccharide, which protects against typhoid fever; however, there are no effective vaccines licensed for use in humans to prevent disease caused by nontyphoidal Salmonella serotypes. Developing a formulation capable of protecting against these diseases remains a challenge for the scientific community. In this work, the reactivity of the sera of mice immunized subcutaneously with formulations based on Outer Membrane Vesicles (OMV) derived from Salmonella Paratyphi A, Salmonella Enteritidis and Salmonella Typhimurium, was evaluated by Western blot, against the respective cell lysates to identify the formulation that induces the best cross immune response. The results obtained indicated a high reactivity of all the sera to the lysates; without an apparent difference between them. Undoubtedly, immunogenicity tests followed by cross-challenge tests should be performed to identify a vaccine candidate. These results suggest that the OMV used in this study are composed of possibly conserved antigens in the three Salmonella serotypes and that they can induce a broad-spectrum immune response and cross protection.

REFERENCES

1. Mezal EH, Sabol A, Khan MA, Ali N, Stefanova R, Khan AA. Isolation and molecular characterization of Salmonella enteric serovar Enteritidis from poultry house and clinical samples during 2010. Food Microbiol.2014;38:67-74. doi: https://10.1016/j.fm.2013.08.003.]

2. Jajere S.M. A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance. Vet World.2019;12(4):504-21. doi: https://10.14202/vetworld.2019.504-521.]

3. MacLennan CA, Martin LB, Micoli F. Vaccines against invasive Salmonella disease: Current status and future directions. Hum Vaccin Immunother.2014;10(6):1478-93. doi: https://10.4161/hv.29054.]

4. Niki M, Shakeel A, Zahid K, Konstantinos CK. Risks, prevalence, and antibiotic resistance of Salmonella in poultry production chain. En: Mares M. Current Topics in Salmonella and Salmonellosis. London, United Kingdom: IntechOpen; 2017. pp. 216-34.]

5. Afema JA, Mather AE, Sischo WM. Antimicrobial resistance profiles diversity in Salmonella from humans and cattle, 2004-2011. Zoonoses Public Health.2015;62 (7):506-17. doi: https://10.1111/zph.12172.]

6. Ao TT, Feasey NA, Gordon MA, Keddy KH, Angulo FJ, Crump JA. Global burden of invasive nontyphoidal Salmonella disease, 2010. Emerg Infect Dis.2015; 21:941-49. doi: https://10.3201/eid2106.140999.]

7. Haeusler GM, Curtis N. Non-typhoidal Salmonella in children: microbiology, epidemiology, and treatment. Adv Exp Med Biol.2013; 764:13-26. doi: https://10.1007/978-1-4614-4726-9_2.]

8. Martin LB, Simon R, MacLennan CA, Tennant SM, Sahastrabuddhe S, Khan MI. Status of paratyphoid fever vaccine research and development. Vaccine.2016;34(26):2900-2. doi: https://10.1016/j.vaccine.2016.03.106.]

9. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature.1970; 227:680-5.]

10. Burnette WN. Western blotting electrophoretic transfer of protein from sodium dodecyl sulphate polyacrylamide get to a modified nitrocellulose and radiographic detection with antibodies and radiodinated protein A. Anal Biochem.1981;112:195-203.]

11. Hamid N, Jain SK. Characterization of an Outer Membrane Protein of Salmonella enterica Serovar Typhimurium That Confers Protection against Typhoid. Clin Vaccine Immunol.2008;15(9):1461-71.]

12. Bai J, Kim SI, Ryu S, Yoon H. Identification and Characterization of Outer Membrane Vesicle-Associated Proteins in Salmonella enterica Serovar Typhimurium. Infect Immun.2014;82(10):4001-10. doi: https://10.1128/IAI.01416-13.]

13. Pérez M, Martínez PA, Pastelin R, Isibasi A, Cunningham AF, Lopez C. La porina OmpD de Salmonella Typhimurium induce altos títulos de anticuerpos de larga duración: implicaciones en el desarrollo de vacunas contra la salmonelosis no tifoídica. Gac Med Mex.2016;152:5-13. Disponible en: https://www.medigraphic.com/pdfs/gaceta/gm-2016/gms162a.pdf.]

14. Yang TC, Ma XC, Liu F, Lin LR, Liu LL, Liu GL, et al. Screening of the Salmonella paratyphi A CMCC 50973 strain outer membrane proteins for the identification of potential vaccine targets. Mol Med Rep.2012;5(1):78-83.]

15. Kisiela D, Laskowska A, Sapeta A, Kuczkowski M, Wieliczko A, Ugorski1 M. Functional characterization of the FimH adhesin from Salmonella enterica serovar Enteritidis. Microbiology.2006;152:1337-46. doi: https://10.1099/mic.0.28588-0.]

16. Liu Q, Tan K, Yuan J, Song K, Li R, Huang X. et al. Flagellin-deficient outer membrane vesicles as adjuvant induce crossprotection of Salmonella Typhimurium outer membrane proteins against infection by heterologous Salmonella serotypes. Int J Med Microbiol.2018;308(7):796-802. doi: https://10.1016/j.ijmm.2018.06.001.]