medigraphic.com
ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2021, Number 1-3

<< Back Next >>

Rev Mex Periodontol 2021; 12 (1-3)

Development of cellulose matrix with antimicrobial properties for application in periodontics

Tovar CKL, Peralta ER, González CRE, Espinosa CLF, Cuevas GJC, Guzmán GDA, Osornio RJL, García CAG, Donohue CA, Valera GE

Language: Spanish
References: 27
Page: 7-11
PDF size: 257.33 Kb.


ABSTRACT

Introduction: The creation and use of different types of biomaterials for tissue rehabilitation and regeneration procedures is an interesting topic today. Cellulose hydrogels are biomaterials used for tissue engineering because of their qualities such as biocompatibility, absence of toxicity and inflammation, they are biodegradable and adsorbed by the body after fulfilling their function, they are easy to process and have an acceptable durability. The use of polymeric structures in tissue regeneration began with the development of membranes of different types of polymers such as PTFE or collagen membranes for dental organ-gum junction regeneration. It is well known that excellent biocompatibility and safety within natural polymers including collagen, chitosan, chitin, starch, and cellulose is a result of their biological characteristics. Objective: The aim of this study was to develop cellulose hydrogels with regenerative purposes added with chlorhexidine digluconate, calcium hydroxide and ozone as an antimicrobial method. Design: Hydrogels were made in four stages that included: 1) washing Agave tequilana Weber fibers; 2) elaboration of the solution; 3) exchange of solvents and 4) microbiological tests. Results: The results obtained experimentally indicate that the proposed design turned out to be effective and efficient to achieve inhibition in vitro. Conclusions: The continuation of the research process with in vivo designs would be one of the ways to avoid the high cost of regenerative treatments in periodontics and thus avoid damage to the budget of our patients.


REFERENCES

  1. Ruozi B, Parma B, Croce MA, Tosi G, Bondioli L, Vismara S et al. Collagen-based modified membranes for tissue engineering: influence of type and molecular weight of GAGs on cell proliferation. Int J Pharm. 2009; 378: 108-115.

  2. Stamatialis DF, Papenburg BJ, Girones M, Saiful S, Bettahalli SNM, Schmitmeier S et al. Medical applications of membranes: drug delivery, artificial organs and tissue engineering. J Membr Sci. 2008; 308: 1-34.

  3. Tovar-Carrillo K, Satoshi Sugita S, Motohiro T, Kobayashi T. Fibroblast compatibility on scaffol hydrogels prepared from Agave tequilana weber bagasse for tissue regeneration. Ind Eng Chem Res. 2013; 52: 11607-11613.

  4. Yamaoka H, Asato H, Ogasawara T, Nishizawa S, Takahashi T, Nakatsuka T et al. Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials. J Biomed Mater Res A. 2006; 78: 1-11.

  5. Li Y, Mai YW, Ye L. Sisal fibre and its composites: a review of recent developments. Compos Sci Technol. 2000; 60: 2037-2055.

  6. Lydon MJ, Minett TW, Tighe BJ. Cellular interactions with synthetic polymer surfaces in culture. Biomaterials. 1985; 6: 396-402.

  7. De Bartolo L, Morelli S, Bader A, Drioli E. Evaluation of cell behaviour related to physico-chemical properties of polymeric membranes to be used in bioartificial organs. Biomaterials. 2002; 23: 2485-2497.

  8. Thomas BH, Craig Fryman J, Liu K, Mason J. Hydrophilic-hydrophobic hydrogels for cartilage replacement. J Mech Behav Biomed Mater. 2009; 2: 588-595.

  9. Chen H, Yuan L, Song W, Wu Z, Li D. Biocompatible polymer materials. Role of protein-surface interactions. Prog Polym Sci. 2008; 33: 1059-1087.

  10. Heinze T, Dicke R, Koschella A, Kull AH, Klohr EA, Koch W. Effective preparation of cellulose derivatives in a new simple cellulose solvent. Macromol Chem Phys. 2000; 201: 627-631.

  11. Nair LS, Laurencin CT. Biodegradable polymers as biomaterials. Progr Polymer Sci. 2007; 32: 762-798.

  12. Gibson LJ. Biomechanics of cellular solids. J Biomech. 2005; 38: 377-399.

  13. Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv. 2011; 29: 322-337.

  14. Striegel AM. Theory and applications of DMAc/LiCL in the analysis of poly-saccharides. Carbohydr Polym. 1997; 34: 267-274.

  15. Cima LG, Vacanti JP, Vacanti C, Ingber D, Mooney D, Langer R. Tissue engineering by cell transplantation using degradable polymer substrates. J Biomech Eng. 1991; 113: 143-151.

  16. Kobayashi T, Tovar-Carrillo KL. Fibroblast cell cultivation on wooden pulp cellulose hydrogels for cytocompatibility scaffold method. Pharm Anal Acta. 2015; 6: 423.

  17. Johansson E, Claesson R, van Dijken JW. Antibacterial effect of ozone on cariogenic bacterial species. J Dent. 2009; 37: 449-453.

  18. Emilson CG. Susceptibility of various microorganisms to chlorhexidine. Scand J Dent Res. 1977; 85: 255-265.

  19. Shah S, Bargale S, Dave BH, Deshpande A, Kariya P, Karri A. Comparison of antimicrobial efficacy of (between) 0.2% chlorhexidine and herbal mouthwash on salivary streptococcus mutans: a randomized controlled pilot study. Clin Dent. 2018; 9 (3): 440-445.

  20. Siqueira JF Jr, Lopes HP. Mechanisms of antimicrobial activity of calcium hydroxide: a critical review. Int Endod J. 1999; 32: 361-369.

  21. Díaz Zúñiga J, Yáñez Figueroa J, Melgar Rodríguez S, Álvarez Rivas C, Rojas Lagos C, Vernal Astudillo R. Virulencia y variabilidad de Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans y su asociación a la periodontitis. Rev Clin Periodoncia Implantol Rehabil Oral. 2012; 5 (1): 40-45.

  22. Carroll IM, Threadgill DW, Threadgill DS. The gastrointestinal microbiome: a malleable, third genome of mammals. Mamm Genome. 2009; 20 (7): 395-403.

  23. Mondragon JD. Endodoncia. México: Nueva Editorial Interamericana; 1995. p. 250.

  24. Vinogradov S. Colloidal microgels in drug delivery applications. Curr Pharm Des. 2006; 12 (36): 4703-4712.

  25. Klawitter JJ, Bagwell JG, Weinstein AM, Sauer BW. An evaluation of bone growth into porous high density polyethylene. J Biomed Mater Res. 1976; 10 (2): 311-323.

  26. Eggli PS, Müller W, Schenk RK. Porous hydroxyapatite and tricalcium phosphate cylinders with two different pore size ranges implanted in the cancellous bone of rabbits. A comparative histomorphometric and histologic study of bony ingrowth and implant substitution. Clin Orthop Relat Res. 1988; (232): 127-138.

  27. Albertson PA. Partition of cell particles and macromolecules. 3rd ed. New York: Wiley; 1986.




2020     |     www.medigraphic.com