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2020, Number 2

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Rev Cubana Plant Med 2020; 25 (2)

Antimicrobial activity of the essential oil from the leaves of Alpinia zerumbet and modulatory effect on conventional antibiotics

Duarte MAC, Dodou LHV, de Morais BAH, Passos SGW, Carneio MS, Pinto NNA

Language: Portugués
References: 24
Page:
PDF size: 148.81 Kb.


ABSTRACT

Introduction: Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm. (Zingiberaceae), popularly known as colony, is intensely used in folk medicine for its antihypertensive, diuretic, spasmolytic, antioxidant, antiulcerogenic, antiinflammatory and antimicrobial actions.
Objective: To evaluate the antimicrobial activity of essential oil extracted from A. zerumbet leaves on reference microbial strains and their potential to modulate the action of commercially available antibiotics.
Methods: Minimum Inhibitory Concentrations (MIC) and Minimum Lethal (MCL) of oil were determined for eleven strains: Pseudomonas aeruginosa ATCC 25619, P. aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538P, S. aureus ATCC 33591, S. aureus ATCC 14458 , S. epidermidis ATCC 12228, Klebsiella pneumoniae ATCC 10031, Enterococcus faecalis ATCC 4083, Escherichia coli ATCC 10536, E. coli ATCC 25922 and Candida albicans ATCC 10231. The modulating effect of the oil on commercial antibiotics was assessed by the modified agar diffusion technique .
Results: A. zerumbet essential oil was able to inhibit 72.7% growth (n = 8) and kill 63.6% (n = 7) of the tested strains with MIC and CLM ranging from 0.312 to 10 mg / ml. The most sensitive strains were S. aureus ATCC 6538P and K. pneumoniae ATCC 10031. In addition, the oil was able to additive to the action of drugs representing the aminoglycosides, cephalosporins, sulfonamides, carbapenems and macrolides classes, and synergistically drugs of the macrolide and polymyxin classes.
Conclusion: Therefore, the essential oil of A. zerumbet shows good antimicrobial activity, with a broad spectrum of action, and is able to potentiate the action of some commercially available drugs. Results demonstrated promising antimicrobial potential of this essential oil that should continue to be investigated, mainly regarding its mechanism of action and toxicity. This work is relevant for scientific validation of essential oil of A. zerumbet leaves pharmacological applications evident in popular medicine and for its use in the development of new therapeutic alternatives effective against bacterial infections.


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