Almeida BJW, Rodrigues FC, Rodrigues CA, Amorim LSM, Pereira MJ, Alves CML, Silva RA

Language: Portugués
References: 23
Page: 1-14
PDF size: 350.79 Kb.

ABSTRACT

Introduction: Tarenaya spinosa (Jacq.) Raf. (Cleomaceae), mussambe, stands out for its strong smell, which is produced by its essential oil. Oils originating from secondary metabolism display biological activities such as insecticidal, antifungal, larvicidal, and others.
Objective: Evaluate the insecticidal and larvicidal activity of oil from T. spinosa leaves against Drosophila melanogaster and Artemia salina.
Method: For insecticidal testing, adult flies were exposed to various oil concentrations (3-30.5 μg/ml). Besides the mortality rate of D. melanogaster, its genotoxic behavior was also evaluated. For larvicidal testing, use was made of the microcrustacean A. salina, which was exposed to oil concentrations ranging from 5 to 1 000 μg/ml for 24 hours accompanied by a positive control (potassium dichromate).
Results: T. spinosa oil displayed insecticidal activity: 6.12 μg/ml caused 50% mortality (CL50) in 24 hours' exposure. Regarding genotoxic behavior, the oil significantly damaged the flight of flies at all the concentrations tested. With respect to larvicidal activity, CL50 was 181.1 μg/ml, showing that the oil has high toxicity.
Conclusions: T. spinosa essential oil has insecticidal potential and may be used to control Diptera. Additionally, the natural product studied was found to exhibit toxicity.

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