Zerpa LR, Huicho L, Náquira C, Espinoza I

Language: English
References: 8
Page: 17-19
PDF size: 58.91 Kb.

ABSTRACT

A simplified method of culturing Blastocystis hominis is described. Two hundred fresh stool samples were inoculated in tubes containing a modified Pavlova’s medium. The tightened tubes were incubated at 36° C without any additional conventional system of anaerobiosis. Cultures were examined after 24, 48 and 72 hours. Overall, 140 (70%) cultures were positive for B. hominis, whereas wet mount examination was positive only in 42 (21%) samples. The different forms of parasite described with the standard method were visualized. This method is proposed as an alternative to the conventional one.

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