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Órgano Oficial del Colegio Mexicano de Alergia, Asma e Inmunología Pediátrica y de la Asociación Latinoamericana de Pediatría

2021, Number 2

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Alerg Asma Inmunol Pediatr 2021; 30 (2)

Relevance of an adequate DNA extraction protocol for molecular studies

Cruz-Enríquez JA, Espinosa-Padilla SE, Medina-Torres EA

Language: Spanish
References: 11
Page: 50-53
PDF size: 128.76 Kb.


ABSTRACT

DNA (deoxyribonucleic acid) extraction has become an important procedure for the molecular study of genetic diseases. For this reason, it is important for both the research physician and the clinician to know the importance of having an adequate DNA extraction protocol and the relevance it has on the concentration, purity and integrity of genomic DNA. In this article we reviewed the factors that influence each step of the DNA extraction protocol to give a suboptimal product that will not allow its subsequent use in any molecular study required, since the protocol is not only confined to the process within the laboratory, recommendations will be given ranging from proper sample collection, the choice of the best anticoagulants to maintain proper preservation conditions of the sample, as well as how long and in what environmental conditions samples can be stored without affecting the outcome of the process. Errors that may occur within the laboratory itself (clinical or research) during the extraction protocol that may affect the purity, quantity, integrity and even the loss of genetic material will be studied, in this article the general steps of the protocol will be described DNA extraction independent of the commercial kit used.


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