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Instituto Nacional de Salud Pública

2020, Número 2

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salud publica mex 2020; 62 (2)


Seguimiento de clonas de Staphylococcus aureus resistentes a meticilina en un hospital en el sur de México

Ortíz-Gil MÁ, Velazquez-Meza ME, Echániz-Aviles G, Mora-Domínguez JP, Carnalla-Barajas MN, Mendiola MEL

Idioma: Ingles.
Referencias bibliográficas: 36
Paginas: 186-191
Archivo PDF: 271.42 Kb.


RESUMEN

Objetivo. Describir las características clínicas y moleculares de cepas de Staphylococcus aureus resistentes a meticilina (SARM) que fueron recolectadas en el Hospital Regional de Alta Especialidad de Veracruz (HRV). Material y métodos. Un total de 107 cepas de SARM fueron analizadas en el presente estudio. Las cepas estudiadas fueron recolectadas de septiembre de 2009 a septiembre de 2010. Las características clínicas y demográficas de los pacientes fueron analizadas; la tipificación molecular de las cepas se hizo por electroforesis en campos pulsados, casete cromosomal estafilococócico (SCCmec, en inglés) y secuenciación de múltiples locus. Resultados. Dos patrones electroforéticos (NY/J y IB) fueron identificados con 4 y 3 subtipos respectivamente. Los aislamientos analizados mostraron dos tipos de SCCmec (I y II) y dos secuencias tipo (ST): ST247 y ST5 relacionados con las clonas Ibérica y Nueva York/Japón respectivamente. Conclusión. Este estudio estableció la presencia en el medio hospitalario de dos linajes clonales de SARM importantes: Nueva York/Japón e Ibérico.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Tacconelli E. Methicillin-resistant Staphylococcus aureus: source control and surveillance organization. Clin Microb Infect. 2009;15(suppl 7):31-8. https://doi.org/10.1111/j.1469-0691.2009.03096.x

  2. Verkaik NJ, Dauwalder O, Antri K, Boubekri I, de Vogel CP, Badiou C, et al. Immunogenicity of toxins during Staphylococcus aureus infection. Clin Infect Dis. 2010;50(1):61-8. https://doi.org/10.1086/648673

  3. Yamamoto T, Hung W, Takano T, Nishiyama A. Genetic nature and virulence of community associated methicillin-resistant Staphylococcus aureus. BioMed. 2013;3(1):2-18. https://doi.org/10.1016/j.biomed.2012.12.001

  4. Peng H, Liu D, Ma Y, Gao W. Comparison of community- and healthcare-associated methicillin-resistant Staphylococcus aureus isolates at a Chinese tertiary hospital, 2012–2017. Sci Rep. 2018;8: 17916. https://doi. org/10.1038/s41598-018-36206-5

  5. Guimarães MA, Ramundo MS, Américo MA, de Mattos MC, Souza RR, Ramos-Júnior ES, et al. A comparison of virulence patterns and in vivo fitness between hospital- and community-acquired methicillin-resistant Staphylococcus aureus related to the USA400 clone. Eur J Clin Microbiol Infect Dis. 2015;34:497-501. https://doi.org/10.1007/s10096-014-2253-1

  6. Vega S, Dowzicky J. Antimicrobial susceptibility among Gram- positive and Gram-negative organisms collected from the Latin American region between 2004 and 2015 as part of the Tigecycline Evaluation and Surveillance Trial. Ann Clin Microbiol Antimicrob. 2017;16:2-16. https://doi. org/10.1186/s12941-017-0222-0

  7. Velazquez MME, Hernández SM, Contreras JF, Pérez CP, Villarreal TL. Surveillance of methicillin-resistant Staphylococcus aureus causing nosocomial infections in five medical centers of Monterrey, Nuevo Leon, Mexico from 2005-2009. Arch Med Res. 2013;44(7):570-4. https://doi.org/10.1016/j. arcmed.2013.09.001

  8. Cornejo JP, Volkow FP, Sifuentes OJ, Echániz AG, Díaz GA, Velázquez AC, et al. Tracing the source of an outbreak of methicillin-resistant Staphylococcus aureus in a tertiary-care oncology hospital by epidemiology and molecular methods. Microb Drug Resist. 2010;16(3):203-8. https://doi. org/10.1089/mdr.2010.0048

  9. Baig S, Johannesen TB, Overballe-Petersen S, Larsen J, Larsen AR, Stegger M. Novel SCCmec type XIII (9A) identified in an ST152 methicillinresistant Staphylococcus aureus. Infect Gen Evol. 2018;61:74-6. https://doi. org/10.1016/j.meegid.2018.03.013

  10. Enright MC, Robinson A, Randle G, Feil EJ, Grundmann H, Spratt BG. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci. 2002;99(11):7687–92. https://doi.org/10.1073/ pnas.122108599

  11. Velazquez MME, Aires de Sousa M, Echániz AG, Solórzano SF, Miranda NG, Silva SJ, et al. Surveillance of methicillin-resistant Staphylococcus aureus in a pediatric hospital in Mexico City during a 7-year period (1997 to 2003): clonal evolution and impact of infection control. J Clin Microbiol. 2004;42(8):3877-80. https://doi.org/10.1128/JCM.42.8.3877-3880.2004

  12. Echániz AG, Velazquez MME, Aires de Sousa M, Morfín OR, Rodríguez NE, Carnalla BN, et al. Molecular characterization of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone in a Mexican hospital (1999-2003). Clin Microbiol Infect Dis. 2006;12(1):12-28. https:// doi.org/10.1111/j.1469-0691.2005.01283.x

  13. Challagundla L, Reyes J, Rafiqullah I, Sordelli D, Echaniz AG, Velazquez MME, et al. Phylogenomic classification and the evolution of clonal complex 5 methicillin-resistant Staphylococcus aureus in the Western Hemisphere. Front Microbiol. 2018;9:1-14. https://doi.org/10.3389/ fmicb.2018.01901

  14. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing: Twentieth Informational Supplement M100-S20. Wayne: CLSI, 2010.

  15. Chung M, Matthews LH, Tomasz A, Adamsson I, Aires de Sousa M, Camou T, et al. Molecular typing of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis: comparison of results obtained in a multilaboratory effort using identical protocols and MRSA strains. Microb Drug Resist. 2000;6(3):189-98. https://doi.org/10.1089/mdr.2000.6.189

  16. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persin DH, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol.1995;33(9):2233-9.

  17. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol. 2000;38:1008-15.

  18. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2002;46(7):2155-61. https://doi.org/10.1128/aac.46.7.2155-2161.2002

  19. Aung MS, Zi H, New KM, Maw WW, Aung MT, Min WW, et al. Drug resistance and genetic characteristics of clinical isolates of staphylococci in Myanmar: high prevalence of PVL among methicillin-susceptible S. aureus belonging to various sequence types. New Microbes New Infect. 2016;10:58-65. https://doi.org/10.1016/j.nmni.2015.12.007

  20. Szymanek MK, Mlynarczyk A, Dobrzaniecka K, Majchrzak K, Mierzwinska NE. Epidemiological and drug-resistance types of methicillin-resistant Staphylococcus aureus strains isolated from surgical and transplantation ward patients during 2010 to 2011. Transplant Proc. 2016;48(5):1414-17. https://doi.org/10.1016/j.transproceed.2016.03.015

  21. Chung M, Dickinson G, de Lencastre H, Tomasz A. International clones of methicillin-resistant Staphylococcus aureus in two hospitals in Miami, Florida. J Clin Microbiol. 2004;42(2):542-7. https://doi.org/10.1128/ JCM.42.2.542-547.2004

  22. Weigel L, Clewell D, Gill S, Clark NC, McDougal LK, Flannagan SE, et al. Genetic analysis of a high-level vancomycin resistant isolate of Staphylococcus aureus. Science. 2003;302(5650):1569-71. https://doi.org/10.1126/ science.1090956

  23. Khokhlova O, Tomita Y, Hung WC, Takano T, Iwao Y, Higuchi W, et al. Elderly infection in the community due to ST5/SCCmecII methicillin resistant Staphylococcus aureus (the New York/Japan clone) in Japan: pantonValentine leukocidin-negative necrotizing pneumonia. J Microbiol Immunol Infect. 2015;48(3):335-9. https://doi.org/10.1016/j.jmii.2012.09.004

  24. Campanile F, Bongiorno D, Borbone S, Stefani S. Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy. Ann Clin Microbiol Antimicrob. 2009;8. https://doi.org/10.1186/1476-0711-8-22

  25. Mato R, Santos SI, Venditti M, Platt DJ, Brown A. Chung M, et al. Spread of the multiresistant Iberian clone of methicillin-resistant Staphylococcus aureus (MRSA) to Italy and Scotland. Microb Drug Resist. 1998;4(2):107- 12. https://doi.org/10.1089/mdr.1998.4.107

  26. Roberts RB, Tennenberg AM, Eisner W, Hargrave J, Drusin LM, Yurt R. Outbreak in a New York City teaching hospital burn center caused by the Iberian epidemic clone of MRSA. Microb Drug Resist. 1998;4(3):175-83. https://doi.org/10.1089/mdr.1998.4.175

  27. Stefani S, Varaldo PE. Epidemiology of methicillin-resistant staphylococci in Europe. Clin Microbiol Infect. 2003;9(12):1179-86. https://doi. org/10.1111/j.1469-0691.2003.00698.x

  28. Heym B, Le Moal M, Armand LL, Nicolas MH. Multilocus sequence typing (MLST) shows that the ‘Iberian’ clone of methicillin-resistant Staphylococcus aureus has spread to France and acquired reduced susceptibility to teicoplanin. J Antimicrob Chemother. 2002;50(3):323-9. https://doi. org/10.1093/jac/dkf132

  29. Krzysztoń RJ, Gniadkowski M, Połowniak PH, Hagmajer E, Hryniewicz W. The first Staphylococcus aureus isolates with reduced susceptibility to vancomycin in Poland. J Antimicrob Chemother. 2002;50:1065-1069. https://doi.org/10.1093/jac/dkf252

  30. Cassone M, Campanile F, Pantosti A, Venditti M, Stefani S. Identification of a variant “Rome clone” of methicillin-resistant Staphylococcus aureus with decreased susceptibility to vancomycin, responsible for an outbreak in an intensive care unit. Microb Drug Resist. 2004;10(1):43-9. https://doi. org/10.1089/107662904323047790

  31. Zribi M, Etienne J, El Euch D, Zribi H, Bes M, Meugnier H, et al. Detection of the first strain of glycopeptides intermediary Staphylococcus aureus in Tunis Rabta hospital. Pathol Biol. 2009;59(6):334-5. https://doi. org/10.1016/j.patbio.2009.10.002

  32. Khanal LK, Adhikari RP, Guragain A. Prevalence of methicillin resistant Staphylococcus aureus and antibiotic susceptibility pattern in a tertiary hospital in Nepal. J Nepal Health Res Counc. 2018; 16(2):172-4. https:// doi.org/10.3126/jnhrcv16i2.20305

  33. Dilnessa T, Bitew A. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus isolated from clinical samples at Yekatit 12 Hospital Medical College, Addis Ababa, Ethiopia. BMC Infect Dis. 2016;16:1-9. https://doi.org/10.1186/s12879-016-1742-5

  34. Conceição T, Aires de Sousa M, Füzi M, Tóth A, Pászti J, Ungvári E, et al. Replacement of methicillin-resistant Staphylococcus aureus clones in Hungary over time: a 10-year surveillance study. Clin Microbiol Infect. 2007;13(10):971-9. https://doi.org/10.1111/j.1469-0691.2007.01794.x

  35. Zaraket H, Otsuka T, Saito K, Dohmae S, Takano T, Higuchi W, et al. Molecular characterization of methicillin-resistant Staphylococcus aureus in hospitals in Niigata, Japan: divergence and transmission. Microbiol Immunol. 2007;51(2):171-6. https://doi.org/10.1111/j.1348-0421.2007.tb03898.x

  36. Mick V, Domínguez MA, Tubau F, Liñares J, Pujol M, Martín R. Molecular characterization of resistance to rifampicin in an emerging hospital-associated methicillin-resistant Staphylococcus aureus clone ST228, Spain. BMN Microbiol. 2010;10:1-8. https://doi.org/10.1186/1471-2180-10-68.




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