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Revista Latinoamericana de Microbiología

2006, Número 1

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Microbiología 2006; 48 (1)


Inhibición mediada por genes cromosomales por Lactobacillus acidophilus AA11 de patógenos intestinales y de alimentos

Abo-Amer AE

Idioma: Ingles.
Referencias bibliográficas: 97
Paginas: 24-30
Archivo PDF: 169.75 Kb.


RESUMEN

Se aislaron aproximadamente 63 cepas de Lactobacillus acidophilus de queso egipcio elaborado en casa y se examinó la producción de antagonismo. Sólo ocho cepas demostraron actividad inhibitoria contra microorganismos que echan a perder el queso (i.e. Staphylococcus aureus y Bacillus cereus) y patógenos (i.e. E. coli, Salmonella sp. y Shigella sp.). Lactobacillus acidophilus AA11 produjo una mayor actividad antimicrobiana con un mayor rango de inhibición. El agente AA11 fue sensible a enzimas proteolíticas y retuvo actividad completa después de 30 min a 100º C. La actividad contra células sensibles fue bactericida pero no bacteriolítica. El compuesto se produjo durante la fase de crecimiento y puede ser extraído de cultivo de fluidos del sobrenadante con n-Butanol. Análisis con 12% SDSPAGE del agente precipitado en sulfato de amonio al 40% mostró dos péptidos con pesos moleculares de ~36 kDa y ~29 kDa. No se identificó ningún plásmido en Lactobacillus acidophilus AA11 indicando que los genes que codifican al agente inhibidor están localizados en el cromosoma. Esas características identifican a la sustancia inhibitoria como un bacteriocín, designado acidocin AA11 y confieren al agente una aplicación potencial como un biopreservador.


REFERENCIAS (EN ESTE ARTÍCULO)

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  29. Muriana, P. M. and Kleanhammer, T. R. 1987. Conjugal transfer of plasmid encoded determinants for bacteriocin production and immunity in Lactobacillus acidophilus 88. Appl. Environ. Microbiol. 53:553-560.

  30. Muriana, P. M. and Kleanhammer, T. R. 1991. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl. Environ. Microbiol. 57:114-121.

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  32. Ouwehand, A. C., Kirjavainen, P. V., Shortt, C., and Salminen, S. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9:43-52.

  33. Piard, J.C. and Desmazeaud, M. 1992. Inhibiting factors produced by lactic acid bacteria. Part 2. Bacteriocins and other antibacterial substances. Lait 72:113-142.

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  35. Salminen, S., Isolauri, E., and Salminen, E. 1996. Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek Int. J. G. Mol. Microbiol. 70:347-358.

  36. Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

  37. Shahani, K. M., and Ayebo, A. D. 1980. Role of dietary lactobacilli in gastrointestinal microecology. Am. J. Clin. Nutr. 33:2448-2457.

  38. Tag, J.R. and McGiven, A.R. 1971. Assay system for bacteriocin. Appl. Microbiol. 21:943.

  39. Tagg, J.R., Dajani, A.S., and Wannamaker, L.W. 1976. Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40:722-756.

  40. Tahar, T., Kanatania, K., Yoshida, K., Miura, H. Sakamoto, M. and Oshimura, M. 1992. Purification and some properties of acidocin 8912, a novel bacteriocin produced by Lactobacillus acidophilus TK8912. Biosci. Biotech. Biochem. 56:1212-1215.

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  44. Biotechnol. 43:165-173.

  45. Tramer, J. 1966. Inhibitory effect of Lactobacillus acidophilus. Nature. 211:204-205.

  46. Upreti, G. C, and Hinsdill, R. D. 1973. Isolation and Characterization of a bacteriocin from a Homofermentative Lactobacillus. Antimicrob Agents Chemother. 4:487-494.

  47. Upreti, G. C. and Hinsdill, R. D. 1975. Production and Mode of Action of Lactocin 27: Bacteriocin from a Homofermentative Lactobacillus. Antimicrob Agents Chemother. 7:139-145.

  48. Vincent, J. G., Veomett, R. C., and Riley, R. F. 1959. Antibacterial activity associated with Lactobacillus acidophilus. J. Bacteriol. 78:477-484.

  49. Zamfir, M., Callewaert, R., Cornea, P. C., Savu, L., Vatafu, I., and De Vuyst, L. 1999. Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB 801. J. Appl. Microbiol. 87:923-931.

  50. Aymerich, M.T., Garriga, M., Monfort, J.M., Nes, I., Hugas, M., 2000. Bacteriocin-producing Lactobacilli in Spanish-style fermented sausages: characterization of bacteriocins. Food Microbiol. 17:33-45.

  51. Barefoot, S. F. and Klaenhammer, T. R. 1983. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl. Environ. Microbiol. 45:1808-1815.

  52. Barefoot, S.F. and Klaenhammer, T. R. 1984. Purification and characterization of the Lactobacillus acidophilus bacteriocins, lactacin B. Antimicrobial Agents and Chemotherapy 26:328-334.

  53. Bergey’s Manual of Systematic Bacteriology, 1984. N. Krieg (Ed), vol. 1, 2, Baltimore: Williams and Wilkins.

  54. Bourlioux, P. 1997. What is currently known about the molecular mechanisms of colonization resistance? Anaerobe 3:179-184.

  55. Chen, H. and Hoover, D.G., 2003. Bacteriocins and their food applications. Comprehensive Rev. Food Sci. Food Safety 2:82-100.

  56. Cuozzo, S. A., Sesma, F., Palacios, J. M., Holgado, A. P. D., & Raya, R. R. 2000. Identification and nucleotide sequence of genes involved in the synthesis of lactocin 705, a two-peptide bacteriocin from Lactobacillus casei CRL 705. FEMS Microbiol. Lett. 185:157-161.

  57. De Klerk, H. C., and Coetzee, J. N. 1961. Antibiosis among lactobacilli. Nature 192:340-341.

  58. De Klerk, H. C., and Smit, J. A. 1967. Properties of a Lactobacillus fermenti bacteriocin. J. Gen. Microbiol. 48:309-316.

  59. De Man, J. C., Rogosa, M. E. and Sharpe, M. E. 1960. A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 23:130-135.

  60. De Vuyst, L. and Vandamme, E. J. 1994. Bacteriocins of Lactic Acid Bacteria: Microbiology, Genetics and Applications, Blackie Academic and Professional, London.

  61. Deraz, S. F., Karlsson, E. N., Hedstrom, M., Andersson, M. M. and Mattiasson, B. 2005. Purification and characterization of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079. J. Biotech. 117:343-354.

  62. Fleming, H. P., Etchells, J. L. and Costilow, R. L. 1975. Microbial inhibition by an isolate of Pediococcus from cucumber brines. Appl. Microbiol. 30:1040-1042.

  63. Fooks, L. J. and Gibson, G. R. 2002. In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol. Ecol. 39:67-75.

  64. Gilliland, S. E., and Speck, M. L. 1977. Use of the Minitek system for characterizing lactobacilli. Appl. Environ. Microbiol. 6:1289-1292.

  65. Hamdan, I. Y, and Mikolajcik, E. M. 1974. Acidolin: an antibiotic produced by Lactobacillus acidophilus. J. Antibiot. (Tokyo). 27:631-636.

  66. Hosono, A., Yastuki, K. and Tokita, F. 1977. Isolation and characterization of an inhibitory substance against Escherichia coli produced by Lactobacillus acidophilus. Milchwissenschaft 32:727-730.

  67. Hurst, A. 1973. Microbial antagonism in foods. Can. Inst. Food Sci. Technol. J. 6:80-90.

  68. Jack, R.W., Tagg, J. R. and Ray, B. 1995. Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59:171–200.

  69. Kanatania, K., Tahara, T., Yoshida, K., Miura, H., Sakamoto, M. and Oshimura, M. 1992. Plasmid-associated bacteriocin production by and immunity of Lactobacillus acidophilus TK8912. Biosci. Biotech. Biochem. 56:648-651.

  70. Kawai, Y., Saitoh, B., Takahashi, O., Kitazawa, H., Saito, T., Nakajima, H. and Itoh, T. 2000. Primary amino acid and DNA sequences ofgassericin T, a lactacin F-family bacteriocin produced by Lactobacillus gasseri SBT2055. Biosci. Biotech. Biochem. 64:2201-2208.

  71. Kitazawa, H., Ino, T., Kawai, Y., Itoh, T., Saito, T., 2002. A novel immunostimulating aspect of Lactobacillus gasseri: induction of ‘Gasserokine’ as chemoattractants for macrophages. Int. J. Food Microbiol. 77:29-38.

  72. Klaenhammer, T. R. 1982. Microbiological considerations in selection and preparation of Lactobacillus strains for uses as dietary adjuncts. J. Dairy Sci. 65:1339-1349.

  73. Klaenhammer, T. R. 1993. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev. 12:39-86.

  74. Klaenhammer, T. R., and Kullen, M. J. 1999. Selection and design of probiotics. International J. Food Microbiol. 50:45-57.

  75. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.

  76. Metchnikoof, E. 1908. The prolongation of life. G.P. Putnam’s Sons, New York.

  77. Morin, A., Saheb, S. A., Bisaillon, J. G., Beaudet, R. and Sylvestre, M. 1980. Effect of culture medium composition on inhibition of growth of Neisseria gonorrhoeae by lactobacilli. Curr. Microbiol. 4:283-286.

  78. Muriana, P. M. and Kleanhammer, T. R. 1987. Conjugal transfer of plasmid encoded determinants for bacteriocin production and immunity in Lactobacillus acidophilus 88. Appl. Environ. Microbiol. 53:553-560.

  79. Muriana, P. M. and Kleanhammer, T. R. 1991. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl. Environ. Microbiol. 57:114-121.

  80. Naidu, A. S., Bidlack, W. R., Clemens, R. A. 1999. Probiotic spectra of lactic acid bacteria (LAB). Crit. Rev. Food Sci. Nutrition 38:13-126.

  81. Ouwehand, A. C., Kirjavainen, P. V., Shortt, C., and Salminen, S. 1999. Probiotics: mechanisms and established effects. Int. Dairy J. 9:43-52.

  82. Piard, J.C. and Desmazeaud, M. 1992. Inhibiting factors produced by lactic acid bacteria. Part 2. Bacteriocins and other antibacterial substances. Lait 72:113-142.

  83. Reid, G., Howard, J., and Gan, B. S. 2001. Can bacterial interference prevent infection? Trends in Microbiol. 9:424-428.

  84. Salminen, S., Isolauri, E., and Salminen, E. 1996. Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie Van Leeuwenhoek Int. J. G. Mol. Microbiol. 70:347-358.

  85. Sambrook, J., Fritsch, E. F. and Maniatis, T. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

  86. Shahani, K. M., and Ayebo, A. D. 1980. Role of dietary lactobacilli in gastrointestinal microecology. Am. J. Clin. Nutr. 33:2448-2457.

  87. Tag, J.R. and McGiven, A.R. 1971. Assay system for bacteriocin. Appl. Microbiol. 21:943.

  88. Tagg, J.R., Dajani, A.S., and Wannamaker, L.W. 1976. Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40:722-756.

  89. Tahar, T., Kanatania, K., Yoshida, K., Miura, H. Sakamoto, M. and Oshimura, M. 1992. Purification and some properties of acidocin 8912, a novel bacteriocin produced by Lactobacillus acidophilus TK8912. Biosci. Biotech. Biochem. 56:1212-1215.

  90. ten Brink, B., Minekus, M, van der Vossen, J.M.B.M., Leer, R. J. and Huis in’t Veld, J. H. J. 1994. Antimicrobial activity of lactobacilli: preliminary characterization and optimization of production of acidocin B, a novel bacteriocin produced by Lactobacillus acidophilus M46. J. Appli. Bacteriol. 77:140-148.

  91. Toba, T., Yoshioka, E. and Itoh, T. 1991. Acidophilucin A, a new heat-labile bacteriocin produced by Lactobacillus acidophilus LAPT 1060. Lett. in Appli Microbiol. 12:106-108.

  92. Todorov, S. D. and Dicks, L. M. T. 2005. Effect of Growth Medium on Bacteriocin Production by Lactobacillus plantarum ST194BZ, a Strain Isolated from Boza. Food Technol. Biotechnol. 43:165-173.

  93. Tramer, J. 1966. Inhibitory effect of Lactobacillus acidophilus. Nature. 211:204-205.

  94. Upreti, G. C, and Hinsdill, R. D. 1973. Isolation and Characterization of a bacteriocin from a Homofermentative Lactobacillus. Antimicrob Agents Chemother. 4:487-494.

  95. Upreti, G. C. and Hinsdill, R. D. 1975. Production and Mode of Action of Lactocin 27: Bacteriocin from a Homofermentative Lactobacillus. Antimicrob Agents Chemother. 7:139-145.

  96. Vincent, J. G., Veomett, R. C., and Riley, R. F. 1959. Antibacterial activity associated with Lactobacillus acidophilus. J. Bacteriol. 78:477-484.

  97. Zamfir, M., Callewaert, R., Cornea, P. C., Savu, L., Vatafu, I., and De Vuyst, L. 1999. Purification and characterization of a bacteriocin produced by Lactobacillus acidophilus IBB 801. J. Appl. Microbiol. 87:923-931.




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