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TIP Revista Especializada en Ciencias Químico-Biológicas

2018, Número S1

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TIP Rev Esp Cienc Quim Biol 2018; 21 (S1)


Perfil de polifenoles, capacidad antioxidante y efecto citotóxico in vitro en líneas celulares humanas de un extracto hidroalcohólico de pétalos de Calendula officinalis L.

Hernández-Rosas NA, García-Zebadúa JC, Hernández-Delgado N, Torres-Castillo S, Figueroa-Arredondo P, Mora-Escobedo R

Idioma: Ingles.
Referencias bibliográficas: 47
Paginas: 54-64
Archivo PDF: 1402.70 Kb.


RESUMEN

Se evaluó el extracto hidroalcohólico de los pétalos de Calendula officinalis, mostrando efectos benéficos contra el estrés oxidativo y enfermedades asociadas a procesos inflamatorios. El contenido de polifenoles del extracto fue determinado por TLC y HPLC-UV-DAD y la capacidad antioxidante se estimó del poder reductor de Fe3+/Fe2+, quelación de Cu2+ y Fe2+, eliminación de radicales •OH, y ensayos ABTS [2, 2´-azino-bis (3 etilbenzotiazolin-6-sulfónico) y DPPH (2,2´difenil-1-picrilhidracilo). La inhibición del crecimiento in vitro (IC50) en células cancerosas humanas de mama (MDAMB- 231), de cuello uterino (HeLa), pulmón (A-549), y colon (HT-29 y Caco-2), fue determinado por el método de MTT. El extracto mostró que el contenido total de polifenoles fue de 37.01±0.015 mg GAe g-1, siendo la rutina la más abundante. Las pruebas ABTS TEAC y DPPH TEAC mostraron una alta capacidad antioxidante. El perfil de actividad antioxidante mostró buena capacidad reductora y capacidad para quelar Cu2+ y Fe2+, así como una buena capacidad de eliminación de radicales •OH. El extracto también mostró efecto citotóxico en células de colon (HT-29 y Caco-2), de mama (MDA-MB-231), de cuello uterino (HeLa) y pulmón (A-549) (IC50 = 10.84, 11.73, 11.26, 11.53 y 11.96 µg mL-1, respectivamente). El análisis de correlación sugiere que los polifenoles de Calendula officinalis están directamente relacionados con la capacidad antioxidante del extracto y se asocian inversamente con la viabilidad celular.


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