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Instituto Nacional de Neurología y Neurocirugía

2017, Número 2

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Arch Neurocien 2017; 22 (2)


Citotoxicidad de nanopartículas metálicas en cultivos de células cerebrales

Manzo RMI, Castro GPB, Agarwal V, Mora LS

Idioma: Español
Referencias bibliográficas: 40
Paginas: 13-23
Archivo PDF: 329.67 Kb.


RESUMEN

Las nanopartículas (NP) se han investigado por su tamaño diminuto y su posibilidad de llegar a sitios específicos. Se estudian materiales para diseñar nanovectores inocuos. Son pocos los estudios de la exposición a diferentes NP que atraviesan la barrera hematoencefálica (BHE) y de los efectos que tienen sobre células en este tejido.
Objetivo: determinar el deterioro celular en presencia de tres diferentes nanopartículas de origen metálico en cultivo de células cerebrales.
Material y métodos: Realizamos un estudio de viabilidad con MTT y morfológico de cultivos cerebrales (glía y neurona) al contacto con concentraciones de: 10, 50 100 y 250 µg/ml de NP de óxido de silicio (NPSiO2), oro recubierto con silicio (NPAu@ Si) y plata (NPAg)
Resultados: las células cerebrales se ven afectadas en su viabilidad y morfología, siendo más aparente la muerte celular con NPAg, con NPSiO2 se observa un descenso en la viabilidad de las mismas pero no tan severo como con NPAg, y para las NPAu@Si, se nota un poco más de resistencia incluso a la concentración más alta.


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