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ISSN 1659-1046 (Impreso)
Odovtos - International Journal of Dental Sciences

2017, Número 3

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Odovtos-Int J Dent Sc 2017; 19 (3)


Estandarización de un protocolo de inmunohistoquímica para detectar microglia del cerebro de ratas de la cepa Wistar

Ramírez Chan K, Fornaguera-Trías J

Idioma: Español
Referencias bibliográficas: 55
Paginas: 45-59
Archivo PDF: 244.34 Kb.


RESUMEN

Objetivo: Estandarizar un protocolo de inmunohistoquímica que se ha utilizado ampliamente en ratones de la cepa C57BL/6J para identificar microglia del sistema nervioso central en ratas de la cepa Wistar. Materiales y Métodos: La presente actividad de investigación se llevó a cabo en dos partes. En la primera parte se implementó un protocolo de inmunohistoquímica para identificar la microglia en el sistema nervioso central de 6 ratas de la cepa Wistar. Se utilizó un anticuerpo primario para rata y un anticuerpo secundario específico para el primario. Una vez establecido el protocolo en cerebro de ratas, se pasó a la segunda parte en donde se produjo un reto inmunológico con la aplicación intraperitoneal de lipopolisacárido en 2 ratas de la cepa Wistar, con el fin de evidenciar los cambios en la morfología de la microglia. Resultados y Discusión: Demostramos que sin realizar grandes modificaciones al protocolo original, este también puede utilizarse para la identificación de microglia en ratas adultas de la cepa Wistar. El establecimiento de este protocolo permitirá estudiar de una mejor manera la interacción bidireccional que se da entre el cerebro y el sistema inmunológico, en condiciones homeostáticas y ante diferentes estímulos fisiológicos y patológicos.


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