Martínez-Flores K, Salazar-Anzures ÁT, Fernández-Torres J, Pineda C, Aguilar-González CA, López-Reyes A

Idioma: Ingles.
Referencias bibliográficas: 59
Paginas: 128-137
Archivo PDF: 512.29 Kb.

RESUMEN

La inmunotransferencia de proteínas, o Western blot, es una técnica molecular semicuantitativa de alta sensibilidad que permite la inmunodetección específica de una proteína o perfil de proteínas. Esta técnica se basa en la separación de proteínas de acuerdo con su carga y peso molecular en geles de poliacrilamida y su posterior transferencia a una membrana sólida, en la que un anticuerpo específico detecta la proteína de interés. Cuatro décadas después de su creación, la técnica de Western blot ha ganado un papel importante en el diagnóstico de varias condiciones médicas, además de constituir una herramienta de cada día en la investigación biomédica.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Eaton SL, Roche SL, Llavero-Hurtado M, Oldknow KJ, Farquaharson C, Gillngwater TH et al. Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting. PLoS One. 2013; 8 (8): e72457. doi: 10.1371/journal.pone.0072457.

2. Yang Y, Ma H. Western blotting and ELISA techniques. Researcher. 2009; 1 (2): 67-86.

3. Kurien BT, Scofield RH. Western blotting. Methods. 2006; 38 (4): 283-293.

4. Jensen EC. The basics of Western blotting. Anat Rec (Hoboken). 2012; 295 (3): 369-371.

5. Kistler J, Duncombe Y, Laemmli UK. Mapping nucleolar proteins with monoclonal antibodies. J Cell Biol. 1984; 99 (6): 1981-1988.

6. Burnette WN. Western blotting: electroporectic transfer of proteins from sodium dodecyl sulfate-polyacrilamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981; 112 (2): 195-203.

7. Towbin H, Staehelin T, Gordo J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 1979; 76 (9): 4350-4354.

8. Chang X, Zhao Y, Sun S, Zhang Y, Zhu Y. The expression of PAD14 in synovium of rheumatoid arthritis. Rheumatol Int. 2009; 29 (12): 1411-1416.

9. Martínez-Maqueda D, Hernández-Ledesma B, Amigo L, Miralles B, Gómez-Ruiz JA. Extraction/fractionation techniques for proteins and peptides and protein digestion. In: Proteomics in foods. Springer US: 2013; pp. 21-50.

10. Johnson M. Detergents: Triton X-100, tween-20, and more. Mater Methods. 2013; 3: 163.

11. Perkel-Jeffrey M. Preparing for your Western blot. Science/AAAS Custom. Publishing Office: Western Blotting: A Guide to current methods 2015; pp. 7-8.

12. MacPhee DJ. Methodological considerations for improving Western blot analysis. J Pharmacol Toxicol Methods. 2010; 61 (2): 171-177.

13. Sapan CV, Lundblad RL, Price NC. Colorimetric protein assay techniques. Biotechnol Appl Biochem. 1999; 29 (2): 99-108.

14. Huang T, Long M, Huo B. Competitive binding to cuprous ions of protein and BCA in the Bicinchoninic Acid Protein Assay. Open Biomed Eng J. 2010; 4: 271-278.

15. García-Arellano H, Vázquez-Duhalt R. Cuantificación de proteínas: una revisión. BioTecnología. 1998; 3: 77-88.

16. Mahmood T, Yang P. Western blot: technique, theory and trouble shooting. N Am J Med Sci. 2012; 4 (9): 429-434.

17. De la Fuente GA, Rodríguez LJ, Fonseca CE. Análisis de proteínas mediante electroforesis e inmunotransferencia (Western blot). Piel. 2007; 22 (5): 252-258.

18. García PHM. Electroforesis en geles de poliacrilamida: fundamentos, actualidad e importancia. Univ Diag. 2000; 1 (2): 31-41.

19. Pérez-Chabela ML, Soriano-Santos J, Ponce-Alquicira E, Díaz-Tenorio LM. Electroforesis en gel de poliacrilamida-SDS como herramienta en el estudio de las proteínas miofribrilares. Una revisión. Nacameh. 2015; 9 (2): 77-96.

20. Cambell MK, Farrell SO. Bioquímica. 4a edición. México, D.F.: Editorial Thomson; 2004.

21. Lomonte BV. Técnicas de laboratorio en inmunología. 3a edición. San José, Costa Rica: Universidad de Costa Rica; 2009.

22. Kurien BT, Scofield RH. Western blotting: methods and protocols. Methods in molecular biology; v. 1312. New York: Springer; 2015: pp. 509.

23. Westermeier R. Electrophoresis in practice. 4th edition. Weinheim, Alemania: Wiley-VCH Verlag GmbH; 2005.

24. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1979; 227 (5259): 680-685.

25. González JM, García E, Fernández JL, Gago L, Benito J. Técnicas analíticas para la detección de gluten en alimentos. Madrid, España: Elecé Industria Gráfica; 2007.

26. Pettegrew CJ, Javini R, Islam MR. Transfer buffer containing methanol can be reused multiple times in protein electrotransfer. J Biomol Tech. 2009; 20 (2): 93-95.

27. Silva JM, McMahon M. The fastest Western in town: a contemporary twist on the classic Western blot analysis. J Vis Exp. 2014; (84): e51149. doi: 10.3791/51149.

28. Eaton SL, Hurtado ML, Oldknow KJ, Graham LC, Marchant TW, Gillingwater TH et al. A guide to modern quantitative fluorescent Western blotting with troubleshooting strategies. J Vis Exp. 2014; (93): e52099. doi: 10.3791/52099.

29. Degasperi A, Birtwistle MR, Volinsky N, Rauch J, Kolch W, Kholodenko BN. Evaluating strategies to normalize biological replicates of Western blot data. PLoS One. 2014; 9 (1): e87293. doi: 10.1371/journal.pone.0087293. eCollection.

30. Sarimehmetoglu HO. Application of Western blotting for the immunodiagnosis of Fasciola hepatica in cattle using excretory/secretory antigens. Turk J Vet Anim Sci. 2002; 26: 1061-1065.

31. Carrillo RT, Moreno G, Márquez J. Análisis de inmunoblot de antígenos de Leishmania infantum y Leishmania donavani en sueros de pacientes con leishmaniasis visceral de Venezuela. Rev Fac Farm. 2012; 54 (1): 21-30.

32. Khammari I, Saghrouni F, Yaacoub A, Meksi SG, Ach H, Garma L et al. IgG western blot for confirmatory diagnosis of equivocal cases of toxoplasmosis by EIA-IgG and fluorescent antibody test. Korean J Parasitol. 2013; 51 (4): 485-488.

33. Magi B, Migliorini L. Western blotting for diagnosis of congenital toxoplasmosis. New Microbiol. 2011; 34 (1): 93-95.

34. Yun LZ, Qin H, Xia HX, Yi J, Zhen G, Mou WY et al. A study of the technique of Western blot for diagnosis of Lyme disease caused by Borrelia afzelli in China. Biomed Environ Sci. 2013; 26 (3): 190-200.

35. Donta ST. Issues in the diagnosis and treatment of Lyme disease. Open Neurol J. 2012; 6: 140-145.

36. Brouqui P, Bacellar F, Bacellar F, Baranton G, Birtles RJ, Bjoersdorff A et al. Guidelines for the diagnosis of tick-borne bacterial diseases in Europe. Clin Microbiol Infect. 2004; 10 (12): 1108-1132.

37. Schumann J, Blessing M. Impact of TGF-β, GM-CSF and antibody response for diagnosis as well as etiopathology of Lyme disease. Scand J Lab Anim Sci. 2011; 38 (3): 177-185.

38. Wongratanacheewin S, Tattawasart U, Lulitanond V, Wongwajana S, Sermswan R, Sookpranee M et al. Characterization of Pseudomonas pseudomallei antigens by SDS-polyacrylamide gel electrophoresis and Western blot. Southeast Asian J Trop Med Public Health. 1993; 24 (1): 107-113.

39. Rattan AS. Western blot analysis of immune response in pulmonary tuberculosis. Ind J Tub. 1991; 38 (1): 11-15.

40. Meyer MP, Eddy T, Baughn RE. Analysis of Western blotting (immunoblotting) technique in diagnosis of congenital syphilis. J Clin Microbiol. 1994; 32 (3): 629-633.

41. Meshkat Z, Roostaee MH, Soleimanjahi H. Development of a Western blot assay for detection of antibodies against HSV using purified HSV virons prepared by sucrose density gradient. Iran J Basic Med Sci. 2009; 11 (4): 215-220.

42. Gad NMA, El-Din TG, Glil RRA, Darwish M. Role of Western blotting in serological diagnosis of Helicobacter pylori infection. Egypt J Med Microbiol. 2008; 17 (2): 169-177.

43. Doiz O, Benito R, Sbihi Y, Osuna A, Clavel A, Gómez-Lus R. Western blot applied to the diagnosis and post-treatment monitoring of human hydatidosis. Diagn Microbiol Infect Dis. 2001; 41 (3): 139-142.

44. Haghpanah B, Mosavat B, Ghayour Z, Oreizi F. Diagnostic value of hydatid cyst antigens using Western blotting method. Jundishapur J Microbiol. 2010; 3 (4): 175-185.

45. Sharma S, Ghosh S, Singh LK, Sarkar A, Malhotra R, Garg OP et al. Identification of autoantibodies against transthyretin for the screening and diagnosis of rheumatoid arthritis. Plos One. 2014; 9 (4): e93905. doi: 10.1371/journal.pone.0093905. eColletion.

46. Balakrishnan L, Bhattacharjee M, Ahmad S, Nirujogi RS, Renuse S, Subbannayya Y et al. Differential Proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients. Clin Proteomics. 2014; 11 (1): 1. doi: 10.1186/1559-0275-11-1.

47. Struglics A, Larsson S, Hansson M, Lohmander LS. Western blot quantification of aggrecan fragments in human synovial fluid indicates differences in fragment patterns between joint diseases. Osteoarthritis Cartilage. 2009; 17 (4): 497-506.

48. Wang Y, Li D, Xu N, Tao W, Zhu R, Sun R et al. Follistatin-like protein 1: a serum biochemical marker reflecting the severity of joint damage in patients with osteoarthritis. Arthritis Res Ther. 2011; 13 (6): R193. doi: 10.1186/ar3522.

49. Wang S, Zhou J, Wei X, Li P, Li K, Wang D et al. Identification of α1-antitrypsin as a potential candidate for internal control for human synovial fluid in Western blot. Rheumatology (Sunnyvale). 2015; Suppl 6: 006.

50. Oliva-Gutiérrez E, Martínez-Godoy MP, Zapata-Zúñiga M, Sánchez-Rodríguez SG. Antígenos reconocidos por el suero de pacientes con artritis reumatoide. Archivos de Medicina. 2012; 8 (2): 1-9. doi: 10.3823/088.

51. Tabushi Y, Nakanishi T, Takeuchi T, Nakajima M, Ueda K, Kotani T et al. Detection of citrullinated proteins in synovial fluids derived from patients with rheumatoid arthritis by proteomics-based analysis. Ann Clin Biochem. 2008; 45 (Pt 4): 413-417.

52. Abdul-Rahman SM, Rashad SM, Doma MA. Human muscle sarcocystosis in relation to non-specific rheumatic diseases and rheumatoid arthritis. Egypt Rheumatol Rehab. 2002; 29 (5): 743-753.

53. Li L, Zhang Z, Wang C, Miao L, Zhang J, Wang J et al. Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma. PLoS One. 2014; 9 (2): e90181. doi: 10.1371/journal.pone.0090181.

54. Liu M, Liu X, Ren P, Li J, Chai Y, Zheng SJ et al. A cancer-related protein 14-3-3ς is a potential inmunodiagnosis of hepatocellular carcinoma. Tumour Biol. 2014; 35 (5): 4247-4256.

55. Li Y, Zhang Q, Penq B, Shao Q, Qian W, Zhang JY. Identification of glutathione S-transferase omega 1 (GSTO1) protein as a novel tumor-associated antigen and its autoantibody in human esophageal squamous cell carcinoma. Tumour Biol. 2014; 35 (11): 10871-10877.

56. Xiong H, Callaghan D, Jones A, Bai J, Rasquinha I, Smith C et al. ABCG2 is up-regulated in Alzheimer’s brain with cerebral amyloid angiopathy and may act as a gatekeeper at the blood-brain barrier for AB1-40 peptides. J Neurosci. 2009; 29 (17): 5463-5475.

57. Morgan C, Jenkins SA, Kynaston HG, Doak SH. The role of adhesion molecules as biomarkers for the aggressive prostate cancer phenotype. PloS One. 2013; 8 (12): 1-7.

58. Cooper ST, Lo HP, North KN. Single section Western blot. Improving the molecular diagnosis of the muscular dystrophies. Neurology. 2003; 61 (1): 93-97.

59. Hernández-San Miguel A, San Miguel R, Martín-Gil FJ. Importancia de las aplicaciones clínicas de la proteómica. Rev Lab Clin. 2010; 3 (1): 40-42.