Mejía-Parra JIJ, Pérez-Araujo MA, Roldán-Rodríguez J, Rojas-Idrogo C, Kato, MJ, Delgado-Paredes GE

Idioma: Español
Referencias bibliográficas: 51
Paginas: 217-232
Archivo PDF: 190.75 Kb.

RESUMEN

Introducción: la infección por Trypanosoma cruzi, conocida como enfermedad de Chagas, es un problema importante de salud pública en países de América Central y Sudamérica.
Objetivo: evaluar la actividad de extractos crudos de acetato de etilo de plantas in vitro de 6-8 meses y 10-12 meses de edad, de tallos leñosos y hojas de plantas silvestres maduras y el lignano tetrahidrofurano grandisina, aislados de Piper solmsianum, sobre las formas epimastigota y tripomastigota de T. cruzi in vitro.
Métodos: en la evaluación del efecto de diversos extractos crudos de acetato de etilo y grandisina de P. solmsianum, sobre la viabilidad de las formas epimastigota y tripomastigota de T. cruzi, se utilizó el método MTT (3-(4,5-dimetiltiazol-2-il)-2,5- difeniltetrazolium bromuro).
Resultados: en la forma epimastigota, el mejor resultado en la inhibición del crecimiento fue obtenido con 50 μg/mL de extracto de tallo y en la forma tripomastigota con 25 y 50 μg/mL de grandisina y plantas in vitro de 6-8 meses de edad, respectivamente. En todos los casos los valores de inhibición oscilaron entre 86 a 96 %. Plantas in vitro de 6-8 meses de edad y grandisina fueron más activas sobre las formas epimastigota y tripomastigota de T. cruzi con valores de CI50 de 0,018 y 0,360 μg/mL, respectivamente.
Conclusiones: se demuestra la actividad tripanocida de extractos de plantas silvestres y plantas in vitro de P. solmsianum.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Urbina JA, Docampo R. Specific chemotherapy of Chagas disease: controversies and advances. Trends in Parasitol. 2003;19:495-501.

2. WHO (World Health Organization). Triatomine bugs. 2007. p. 213. [cited 2015 Aug 12]. Available from: http://www.who.int/entity/water_sanitation_health/resources/vector210to222.pdf

3. Organización Mundial de la Salud. Control de la enfermedad de Chagas: segundo informe del comité de expertos de la OMS. Ginebra: OMS; 2003. Serie de Informes Técnicos 905.

4. Berger I, Barrientos AC, Cáceres A, Hernández M, Rastrelli L, Passreiter CM, et al. Plants used in Guatemala for the treatment of against Trypanosoma cruzi. J Ethnopharmacol. 1998;62:107-15. protozoal infections. II. Activity of extracts and fractions of five Guatemala plants

5. Uchiyama N. Antichagasic activities of natural products against Trypanosoma cruzi. J Health Sci. 2009;55:31-9.

6. Nakajima-Shimada J, Hirota Y, Aoki T. Inhibition of Trypanososma cruzi growth in mammalian cells by purine and pyrimidine analogs. Antimicrob Agents Ch. 1996;40:2455-8.

7. Prata A. Clinical and epidemiological aspects of Chagas disease. Lancet Infect Dis. 2001;1:92-100.

8. Tyler KM, Engman DM. The life cycle of Trypanosoma cruzi revisited. Int J Parasitol. 2001;31:472-1.

9. Carrada-Bravo T. Trypanosoma cruzi: Historia natural y diagnóstico de la enfermedad de Chagas. Rev Mex Patol Clin. 2004;51:205-19.

10. Paulino M, Iribarne F, Dubin M. Aguilera-Morales S, Tapia O, Stoppani AO. The chemoterapy of Chagas' disease: an overview. Mini Rev Med Chemi. 2005; 5: 499- 519.

11. Da Silva CF, Batista D da G, Oliveira GM, de Souza EM, Hammer ER, da Silva PB, et al. In vitro and in vivo investigation of the efficacy of arylimidamide DB1831 and its mesylated salt form―DB1965―against Trypanosoma cruzi infection. PLoS One. 2012;7:e30356.

12. Maya JD, Repetto Y, Agosin M, Ojeda JM, Tellez R, Gaule C, et al. Effects of nifurtimox and benznidazole upon glutatione and trypanothione content in epimastigote, trypomastigote and amastigote forms of Trypanosoma cruzi. Mol Biochem Parasit. 1997;86:101-6.

13. Veloso VM, Carneiro CM, Toledo MJO, Chiari E, Tafuri WL, Bahia MT. Variation in susceptibility to benznidazole in isolates derived from Trypanosoma cruzi parenteral strains. Mem Inst Oswaldo Cruz. 2001;96:1005-11.

14. Schinella G, Tournier H, Zaidenberg A. In vitro andin vivo activity of berberine on the blood trypomastigote from Trypanosoma cruzi. Bol Latinoam Caribe Plant Med Aromat. 2007;6:81-5.

15. Lockman JW, Hamilton AD. Recent developments in the identification of chemotherapeutics for Chagas disease. Curr Med Chem. 2005;12:945-59.

16. Maya JD, Cassels BK, Iturriaga-Vásquez P, Ferreira J, Faúndez M, Galanti N, et al. Mode of action of natural and synthetic drugs against Trypanosoma cruzi and their interaction with the mammalian host. Comp Biochem Physiol, Part A. 2007;146:601-20.

17. Duschak VG, Couto AS. An insight on targets and patented drugs for chemotherapy of Chagas disease. Recent Pat Antiinfect Drug Discov. 2007;2:19-51.

18. Monteiro D, Guimaraes EF. Flora do Parque Nacional do Itatiaia - Brasil: Manekia e Piper (Piperaceae). Rodriguesia. 2009;60:999-1024.

19. Kato MJ, Furlan M. Chemistry and evolution of the Piperaceae. Pure Appl Chem. 2007;79:529-38.

20. de Oliveira A, Mesquita JT, Tempone AG, Lago JHG, Guimarães EF, Kato MJ. Leishmanicidal activity of an alkenylphenol from Piper malacophyllum is related to plasma membrane disruption. Exp Parasitol. 2012;132:383-7.

21. Martins RCC, Lago JHG, Albuquerque S, Kato MJ. Trypanocidal tetrahydrofuran lignans from inflorescences of Piper solmsianum. Phytochemistry. 2003;64:667-70.

22. Ribeiro TS, Freire-de-Lima L, Previato JO, Mendonça-Previato L, Heise N, de Lima MEF. Toxic effects of natural piperine and its derivatives on epimastigotes and amastigotes of Trypanosoma cruzi. Bioorg Med Chem Lett. 2004;14:3555-8.

23. Luize PS, Tiuman TS, Morello LG, Maza PK, Ueda-Nakamura T, Dias Filho BP, et al. Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Braz J Pharm Sci. 2005;41:85-94.

24. Luize PS, Ueda-Nakamura T, Dias BP, Cortez DAG, Nakamura CV. Activity of neolignans isolated from Piper regnellii (Mig.) C. DC. var. pallescens (C. DC.) Yunck. against Trypanosoma cruzi. Biol Pharm Bull. 2006;29:2126-30.

25. Felippe LG, Baldoqui DC, Kato MJ, Bolzani VD, Guimarães EF, Cicarelli RMB, et al. Trypanocidal tetrahydrofuran lignans from Peperomia blanda. Phytochemistry. 2008;69:445-50.

26. Regasini LO, Cotinguiba F, Passerini GD, Bolzani VS, Cicarelli RMB, Kato MJ, et al. Trypanocidal activity of Piper arboreum and Piper tuberculatum (Piperaceae). Braz J Pharmacog. 2009;19:199-203.

27. Flores N, Jimenez IA, Gimenez A, Ruiz G, Gutierrez D, Bourdy G, et al. Antiparasitic activity of prenylated benzoic acid derivatives from Piper species. Phytochemistry. 2009;70:621-7.

28. Mota JD, Leite AC, Batista JM, Lopez SN, Ambrosio DL, Passerini GD, et al. In vitro trypanocidal activity of phenolic derivatives from Peperomia obtusifolia. Planta Med. 2009;75:620-3.

29. Cotinguiba F, Regasini LO, Bolzani VD, Debonsi HM, Passerini GD, Cicarelli RMB, et al. Piperamides and their derivatives as potential anti-trypanosomal agents. Med Chem Res. 2009;18:703-11.

30. Pelizzaro-Rocha K, Veiga-Santos P, Rocha K, Silva S, Ueda-Nakamura T, Dias B, et al. The trypanocidal action of eupomatenoi-5 isolated from Piper regnellii var pallescens may be related an imbalance between the antioxidant system and ROS. Planta Med. 2010;76:479.

31. Batista JM, Batista ANL, Rinaldo D, Vilegas W, Ambrosio DL, Cicarelli RMB, et al. Absolute configuration and selective trypanocidal activity of gaudichaudianic acid enantiomers. J Nat Prod. 2011;74:1154-60.

32. Veiga-Santos P, Desoti VC, Miranda N, Ueda-Nakamura T, Dias-Filho BP, Silva SO, et al. The natural compounds piperovatine and piperlonguminine induce autophagic cell death on Trypanosoma cruzi. Acta Trop. 2013;125:349-56.

33. Esperandim VR, Ferreira DS, Rezende KCS, Cunha WR, Saraiva J, Bastos JK, et al. In vivo infection by Trypanosoma cruzi: a morphometric study of tissue changes in mice. Exp Parasitol. 2013;133:442-6.

34. Yuncker TG. The Piperaceae of Brazil II: Piper-Group V; Ottonia, Pothomorphe, Sarcorhachis. Hoehnea. 1973;3:29-284.

35. Murashige T, Skoog F. A revised medium for rapid growth a bio assays with tobacco tissue cultures. Physiol Plant. 1962;15:473-97.

36. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Inmunol Methods. 1983;65:55-63.

37. Sánchez-Burgos G, Mezquita-Vega RG, Escobedo-Ortegón J, Ramírez-Sierra MJ, Arjona-Torres A, Ouaissi A, et al. Comparative evaluation of therapeutic DNA vaccines against Trypanosoma cruzi in mice. FEMS Immunol Med Microbiol. 2003;50:333-41.

38. Coura JR, Castro SL. A critical review on Chagas disease chemoterapy. Mem Inst Oswaldo Cruz. 2002;97:3-24.

39. Balbuena TS, Santa-Catarina C, Silveira V, Kato MJ, Floh EIS.In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae). Acta Bot Bras. 2009;23:274-81.

40. Abe F, Nagafuji S, Yamaguchi T, Okabe H, Maki J, Higo H, et al. Trypanocidal constituents in plants 1. Evaluation of some mexican plants for their trypanocidal activity and active constituents in guaco, roots of Aristolochia taliscana. Biol Pharm Bull. 2002;25:1188-91.

41. Calderón AI, Romero LI, Ortega-Barría E, Solis PN, Zacchino S, Gimenez A, et al. Screening of Latin American plants for antiparasitic activities against malaria, Chagas disease and leishmaniasis. Pharm Biol. 2010;48:545-53.

42. Alves RT, Regasini LO, Funari CS, Young MCM, Rimoldi A, Bolzani VS, et al. Trypanocidal activity of Brazilian plants against epimastigote forms from Y and Bolivia strains of Trypanososma cruzi. Braz J Pharmacog. 2012;22:528-33.

43. Muñóz OM, Maya JD, Ferreira J, Christen P, San Martin J, López-Muñoz R, et al. Medicinal plants of Chile: Evaluation of their anti-Trypanososma cruzi activity. Z. Naturforsch. 2013;68:198-202.

44. Polanco-Hernández G, Escalante-Erosa F, García-Sosa K, Acosta-Viana K, Chan- Bacab M, Sagua-Franco H. In vitro and in vivo trypanocidal activity of native plants from the Yucatan Peninsula. Parasitol Res. 2012;110:31-5.

45. Danelutte AP, Costantin MB, Delgado GE, Braz-Filho R, Kato MJ. Divergence of secondary metabolism in cell suspenison cultures and differentiated plants of Piper cernuum and P. crassinervium. J Braz Chem Soc. 2005;16:1425-30.

46. Delgado-Paredes GE, Kato MJ, Rojas-Idrogo C. Suspensiones celulares y producción de metabolitos secundarios en cultivos in vitro de Piper sp. Bol Latinoam Caribe Plant Med Aromat. 2013;12:269-82.

47. Vanisree M, Lee YC, Lo SF, Nalawade SM, Lin CF, Tsay HS. Studies on the production of some important secondary metabolites from medicinal plants by tissue culture. Bot Bull Acad Sin. 2004;45:1-22.

48. Bobadilla M, Zavaleta F, Sisniegas M, Zavaleta G, Mostacero J, Taramona L. Evaluación larvicida de suspensiones celulares acuosas de A. muricata Linaeus "guanábana" sobre Aedes aegypti Linaeus. Rev Peru Biol. 2005;12:145-52.

49. Leatemia J, Isman B. Toxicity and antifeedant activity of crude seed extracts of Annona squamosa (Annonaceae) against lepidoptera pests and natural enemies. Int J Trop Insect Sci. 2004;24:150-8.

50. Navickiene HMD, Miranda JE, Bartoli SA, Kato MJ, Bolzani VS, Furlan M. Toxicity of extracts and isobutyl amides from Piper tuberculatum: potent compounds with potential for the control of the velvetbean caterpillar, Anticarsia gemmatalis. Pest Manag Sci. 2007;63:399-403.

51. Campos M, Salomão K, Castro-Pinto D, León L, Barbosa H, Maciel M, et al. Croton cajucara crude extract and isolated terpenes: activity on Trypanosoma cruzi. Parasitol Res. 2010;107:1193-1204.