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2015, Número 1

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Rev Mex Ing Biomed 2015; 36 (1)


Efecto de implantes de polipirrol sintetizados por diferentes métodos sobre lesiones de médula espinal en ratas

Álvarez-Mejía L, Salgado-Ceballos H, Olayo R, Cruz GJ, Olayo MG, Díaz-Ruiz A, Ríos C, Mondragón-Lozano R, Morales-Guadarrama A, Sánchez-Torres S, Morales J

Idioma: Ingles.
Referencias bibliográficas: 52
Paginas: 7-21
Archivo PDF: 1015.64 Kb.


RESUMEN

En el presente trabajo se comparó el efecto de implantes poliméricos derivados del pirrol (polipirrol o PPy) y del copolímero polipirrol/polietilenglicol (PPy/PEG), obtenidos por diferentes métodos de síntesis: químico, electroquímico y polimerización por plasma con el propósito de determinar si el método de síntesis puede influir sobre el efecto que producen al ser implantados después de una lesión traumática de la médula espinal de ratas. Antes de realizar el implante, las características químicas y estructurales de los polímeros fueron analizadas por espectroscopia de infrarrojo (IR). Se utilizó un modelo experimental de lesión traumática de médula espinal (LTME) por sección completa en ratas. La LTME se realizó a nivel torácico 9 y el polímero fue implantado de inmediato en la zona de lesión. La recuperación de la función motora se evaluó mediante la escala Basso, Beattie y Bresnahan (BBB) una vez por semana durante 5 semanas. La evaluación histológica se realizó al término del seguimiento con la tinción de hematoxilina/eosina. Los resultados muestran que los animales implantados con polímeros sintetizados por plasma se integraron mejor al tejido nervioso, redujeron la respuesta inflamatoria y favorecieron una mayor recuperación funcional en comparación con los animales implantados con materiales sintetizados por métodos químicos o electroquímicos.


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