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2015, Número 4

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Rev Mex Patol Clin Med Lab 2015; 62 (4)


Análisis genómico de las funciones proteicas y relaciones taxonómicas de bacterias causantes de enfermedad diarreica aguda

Moya-Salazar JJ, Ubidia-Incio RA

Idioma: Español
Referencias bibliográficas: 42
Paginas: 206-219
Archivo PDF: 480.69 Kb.


RESUMEN

Se diseñó un estudio analítico-correlacional de corte transversal para analizar los genomas de bacterias causantes de la enfermedad diarrea aguda (EDAs) y se establecieron relaciones funcionales entre funciones proteicas con base en el análisis realizado con las herramientas COGs data base (http://www.ncbi.nlm.nih.gov/COG/) y TaxPlot (http://www.ncbi.nlm. nih.gov/sutils/taxik2.cgi?isbact=1). Se realizó el conteo de las categorías de los COGs para cada especie, la determinación del porcentaje real de cada categoría respecto a la cantidad total de COGs, un análisis comparativo interproteico de cada especie mediante TaxPlot y la clasificación filogenética de las proteínas codificadas en genomas completos utilizando el algoritmo UPGMA. Se incluyó un total de 15 microorganismos (9 bacterias causantes de EDAs y 6 bacterias saprofi tas). 11 de las 15 bacterias presentaron mayor COGs en las categorías J –12.25%– (6 bacterias causantes de EDAs y 5 bacterias saprófitas). Las categorías funcionales para metabolismo fueron uniformes y las categorías funcionales Y (0.00%), Z (0.03%), B (0.01%) y A (0.05%) fueron las de menor cuantía. Los COG pueden extrapolarse en datos exactos que describen el perfil proteico de los agentes patógenos. El método filogenético por TaxPlot reduce el tiempo de análisis y representa una aproximación holística al estudio de las relaciones filogenéticas a nivel proteómico.


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