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2015, Número 1

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Rev Med UV 2015; 15 (1)


Cultivos clonogénicos: historia y su uso actual como control de calidad pre-trasplante

Alcántara L, Bonilla-Cisneros C, Luna F

Idioma: Español
Referencias bibliográficas: 39
Paginas: 6-12
Archivo PDF: 481.83 Kb.


RESUMEN

El trasplante de células progenitoras hematopoyéticas se utiliza actualmente para el tratamiento de enfermedades onco-hematológicas como leucemias y linfomas. Una técnica utilizada para evaluar la capacidad de proliferación in vitro de las células progenitoras hematopoyéticas colectadas en muestra de sangre periférica, sangre periférica movilizada, médula ósea, y sangre de cordón umbilical es el cultivo clonogénico, en el cuál se forman colonias de células de granulocitos-eritroidesmacrófagos- megacariocitos (UFC-GEMM), unidades formadoras de colonias de granulocitos-macrófagos (UFC-GM), unidades formadoras de eritroides (UFC-E) en un medio de metilcelulosa. El objetivo de este artículo es dar una breve descripción histórica de los cultivos clonogénicos, así como resaltar la importancia de su utilización en el control pre-trasplante para enfermedades onco-hematológicas; esto es utilizado como un medio de calidad y seguridad para el paciente trasplantado.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Gluckman E, Broxmeyer HE, Auerbach AD, et al. Hematopoietic reconstitution in a patient with Fanconi´s anemia by means of umbilical-cord blood from an HLA-identical sibling. New Eng. J. Med. 1989: 1174-8.

  2. Lumley M. A, Burton A, Billingham LJ, McDonald DF, Czarnecka HM, Milligan DW. Quality assurance of CFU-GM assays: interlaboratory variation despite standard reagents. Eur J Haematol. 1999 Jan;62(1):32-7.

  3. Martínez-Jaramillo G, Vela-Ojeda J, Sánchez-Valle E, Montesinos JJ, Mayani H. In vitro functional alterations in the hematopoietic system of adult patients with acute lymphoblastic leukemia. 2007:83-9.

  4. Verena Reimann, Ursula Creutzig, Gesine Kögler. Stem Cells Derived From Cord Blood in Transplantation and Regenerative Medicine. Dtsch Arztebl Int. 2009:831–836.

  5. Bonifer C. Epigenetic plasticity of hematopoietic cells. Cell Cycle. 2005: 211-214.

  6. http://stemcells.nih.gov

  7. Boylan M. Galen: On blood, the pulse and the arteries. J Hist Biol; 2007:207-30.

  8. Hajdu SI. A note from history: The first two laboratory scientists. Ann Clin Lab Sci 2003: (33) 438–40.

  9. Keynes G. Blood transfusion. London: John Write and Sons. 2010:1- 19.

  10. Starr D. Blood: An epic history of medicine and commerce. New York: Perennial (HarperCollins), 2003: 31-52.

  11. Doyle k. Blood component preservation a storage. In: Rudmann Sv. Ed Textbook of blood banking and transfusion medicine. Philadelphia. Elsevier Sounders, 2005:258-80.

  12. Ficarra B J. The evolution of blood transfusion. Ann Med Hist. 1942:305-6.

  13. Ernest Beutler M.D., Marshall A. Lichtman M.D., Barry S. Coller M.D., Thomas J. Kipps M.D. Ph.D., Uri Seligsohn M.D. (Editor) Structure of the marrow and the hematopoietic environment. McGraw-Hill Professional. Williams Hematology 6th edition. November 28, 2000.

  14. Borell M. “Brown-Séquard’s organotherapy and its appearance in America at the end of the nineteenth century”. Bull Hist Med.1976: 309–320.

  15. Martín Gilbert. Segunda Guerra Mundial 1939-1945, La V. i. Editorial Edaf. 2005. 696pp. ISBN: 8497342984

  16. Leonard J. Cole, Maurice C. Fishler, and Victor P. Bond. Subcellular fractionation of mouse spleen radiation protection activity. Proc Natl Acad Sci U S A. 1953: 759–772.

  17. Jacobson, L. O., Simmons, E. J., Marks, E. K., and Eldredge, J. H., Recovery from irradiation injure. Science 1951: 510-511.

  18. Till E, McCulloch JA. Direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Research.1961:213-222.

  19. Becker A. J, McCulloch E A & Till J E. Cytological demonstration of the clonal nature of spleen colonies derived from transplanted mouse marrow cells. Nature. 1963:452-4.

  20. Bellingham RE, Brent L, and Medawar PB. Activity acquired tolerance of foreign cells. Nature. 1963; 172, 603-606.

  21. Ono S J J. The birth of transplantation immunology: the Billingham- Medawar experiments at Birmingham Universit College London. 1951. Exp Biol 2004: 4013-4014.

  22. Ginsburg H, Sachs L. The long term cultivation in tissue culture of leukemic cells from mouse leukemia induced by Moloney virus or by x rays. J Nat. Cancer Inst. 1961:53-71.

  23. Ginsburg H, Sachs L. Formation of pure suspensions of mast cells in tissue culture by differentiation of lymphoid cells from the mouse thymus. J Nat Cancer Inst. 1963; 31:1-40

  24. Pluznik DH, Sachs L. The cloning of normal “mast” cells in tissue culture. J Cell Comp Physiol. 1965; 66:319-24.

  25. Hotchin J. E. Use of methyl cellulose gel as a substitute for agar in tissue-culture overlays. Nature 175; 1955: 352

  26. Ichikawa Y, Pluznik DH, Sachs L. In vitro control of the development of macrophage and granulocyte colonies. Proc Nat Acad Sci US. 1966; 56:488-95

  27. Pluznik D H & Sachs L. The cloning of normal “mast” cells in tissue culture. Cell. Comp. Physiol 1965; 66:319-24. [Citation Classic. Current Contents/Life Sciences 1983; 39:18.

  28. Iscove N, Senn J, Till J, McCulloch E. Colony formation by normal and leukemic human marrow cells in culture: effect of conditioned medium from human leukocytes. Blood 1971; 37:1-5. Citation Classic. Current Contents/Life Sciences.1983; 23:151.

  29. Pike B L & Robinson W A. Human bone marrow colony growth in agargel. J. Cell. Physiol. 1970; 76:77-84.

  30. Hoang T, Iscove N. N.& Odartchenko N. Agar extract induces release of Paran M, Sachs L, Barak Y, Resnitzky P. In vitro induction of granulocyte differentiation in hematopoietic cells from leukemic and non-leukemic patients. Proc. Nat Acad Sci US. 1970; 67:1542-9.

  31. Kinzfogl JM, Broxmeyer HE. Brief historical overview of hematology, cord blood, and links between the nervous and hematopoietic systems. In: Broxmeyer H. E, ed. Cord Blood: Biology, Transplantation, Banking, and Regulation Bethesda, MD Press, 2011; 1:1-16

  32. Ploemacher RE, van der Sluijs JP, Voerman JS et al. An in vitro limiting dilution assay of long-term repopulating hematopoietic stem cells in the mouse. Blood. 1989; 74, 2755-2763

  33. Majeti R, Park CY, and Weissman IL. Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood. Stem Cell; 2007: 1, 635-645.

  34. Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH. Antigenic analysis of hematopoiesis. III. An hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. J Immunol. 1984; (33):157–65.

  35. Human Colony-Forming Cell (CFC) Assays using Methocult®. Technical Manual. V. 3.1.0. 2009. Stem Cell Technologies ®.

  36. Coulombel L. Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assays. Oncogene. 2004; (23): 7210-22.

  37. Dobo I, Pineau D, Robillard N, Geneviève F, Piard N, Zandecki M, y Hermouet S. Standardization of the CFU-GM Assay: Advantages of Plating a Fixed Number of CD34+ Cells in Collagen Gels. Journal of Hematotherapy & Stem Cell Research. 2003;12(5): 543-551.

  38. Kurita N, Frassoni F, Chiba S, Podestà M. Impact of length of cryopreservation and origin of cord blood units on hematologic recovery following cord blood transplantation. Bone Marrow Transplant. 2015 Mar 23. doi: 10.1038/bmt.2015.56

  39. Jo-Anna Reems, Karen M. Hall, Luladay H. Gebru, Greta Taber, Ivan N. Rich. Development of a novel assay to evaluate the functional potential of umbilical cord blood progenitors. Transfusion. 2008; (48): 620-628.




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