medigraphic.com
ISSN 1027-2852 (Digital)
ISSN 0864-4551 (Impreso)

2014, Número 4

<< Anterior Siguiente >>

Biotecnol Apl 2014; 31 (4)


Colección de cepas de Salmonella typhimurium para la evaluación toxicológica y anti-genotoxicológica

Cuétara EB, Sánchez-Lamar A, Hernández-Guadarrama BE, Espinosa-Aguirre JJ, Camacho-Carranza R

Idioma: Ingles.
Referencias bibliográficas: 57
Paginas: 278-284
Archivo PDF: 432.74 Kb.


RESUMEN

La recombinación homóloga es un mecanismo de reparación del ADN que adicionalmente genera diversidad biológica. Casi todo nuestro conocimiento acerca de ella proviene de ensayos que emplean extremos de doble cadena como iniciadores de recombinación. En este trabajo se evaluó la recombinación homóloga en bacterias, mediante un ensayo de segregación de duplicaciones cromosomales que mide los intercambios entre cadenas de cromátidas hermanas sin favorecer ninguna ruta de reparación. Se construyó una colección de cepas de Salmonella enterica serovar typhimurium deficiente en genes que codifican proteínas involucradas en la recombinación homóloga. Se evaluó las tasas de segregación espontáneas (SSR) e inducidas por luz ultravioleta (UV-ISR). Se demostró que la ausencia de la resolvasa RuvC no afecta la SSR, mientras que los defectos en RecA, RecQ y RecB/RecF la disminuyen y la carencia de SbcCD o RuvAB la estimulan. En mutantes nulos para RecB, las lesiones que usualmente no son detectadas por el sistema RecFOR parecen ser reconocidas y reparadas por esta vía. La metodología usada permitió corroborar la existencia de una ruta independiente de RecA en Salmonella y sugiere la existencia de rutas alternativas de recombinación para el doble mutante recB/recF.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Blackwood JK, Rzechorzek NJ, Bray SM, Maman JD, Pellegrini L, Robinson NP. End-resection at DNA double-strand breaks in the three domains of life. Biochem Soc Trans. 2013;41(1):314-20.

  2. El-Bibany AH, Bodnar AG, Reinardy HC. Comparative DNA damage and repair in echinoderm coelomocytes exposed to genotoxicants. PloS one. 2014;9(9):e107815.

  3. Norval M, Lucas RM, Cullen AP, de Gruijl FR, Longstreth J, Takizawa Y, et al. The human health effects of ozone depletion and interactions with climate change. Photochem Photobiol Sci. 2011;10(2):199-225.

  4. Yagura T, Makita K, Yamamoto H, Menck CF, Schuch AP. Biological sensors for solar ultraviolet radiation. Sensors. 2011;11(4):4277-94.

  5. Sutherland JC. Repair-dependent cell radiation survival and transformation: an integrated theory. Phys Med Biol. 2014;59(17):5073-90.

  6. Pascucci B, D’Errico M, Parlanti E, Giovannini S, Dogliotti E. Role of nucleotide excision repair proteins in oxidative DNA damage repair: an updating. Biochemistry (Mosc). 2011;76(1):4-15.

  7. Janowska B, Komisarski M, Prorok P, Sokolowska B, Kusmierek J, Janion C, et al. Nucleotide excision repair and recombination are engaged in repair of trans-4-hydroxy-2-nonenal adducts to DNA bases in Escherichia coli. Int J Biol Sci. 2009;5(6):611-20.

  8. Cox MM. Recombinational DNA repair of damaged replication forks in Escherichia coli: questions. Annu Rev Genet. 2001;35:53-82.

  9. Galitski T, Roth JR. Pathways for homologous recombination between chromosomal direct repeats in Salmonella typhimurium. Genetics. 1997;146(3):751-67.

  10. Espinosa-Aguirre J, Barajas-Lemus C, Hernandez-Ojeda S, Govezensky T, Rubio J, Camacho-Carranza R. RecBCD and Rec- FOR dependent induction of chromosomal deletions by sodium selenite in Salmonella. Mutat Res. 2009;665(1-2):14-9.

  11. Luria SE, Delbruck M. Mutations of bacteria from virus sensitivity to virus resistance. Genetics. 1943;28(6):491-511.

  12. Aravind L, Makarova KS, Koonin EV. Survey and summary: Holliday junction resolvases and related nucleases: identification of new families, phyletic distribution and evolutionary trajectories. Nucleic Acids Res. 2000;28(18):3417-32.

  13. Saintigny Y, Makienko K, Swanson C, Emond MJ, Monnat RJ, Jr. Homologous recombination resolution defect in werner syndrome. Mol Cell Biol. 2002;22(20):6971-8.

  14. Baharoglu Z, Bradley AS, Le Masson M, Tsaneva I, Michel B. ruvA Mutants that resolve Holliday junctions but do not reverse replication forks. PLoS Genet. 2008;4(3):e1000012.

  15. Giraud-Panis MJ, Lilley DM. Structural recognition and distortion by the DNA junction-resolving enzyme RusA. J Mol Biol. 1998;278(1):117-33.

  16. Zechiedrich L, Osheroff N. Topoisomerase IB-DNA interactions: X marks the spot. Structure. 2010;18(6):661-3.

  17. Connelly JC, de Leau ES, Leach DR. DNA cleavage and degradation by the SbcCD protein complex from Escherichia coli. Nucleic Acids Res. 1999;27(4):1039-46.

  18. Palchevskiy V, Finkel SE. A role for single-stranded exonucleases in the use of DNA as a nutrient. J Bacteriol. 2009;191(11):3712-6.

  19. Eykelenboom JK, Blackwood JK, Okely E, Leach DR. SbcCD causes a double- strand break at a DNA palindrome in the Escherichia coli chromosome. Mol Cell. 2008;29(5):644-51.

  20. Miesel L, Roth JR. Salmonella recD mutations increase recombination in a short sequence transduction assay. J Bacteriol. 1994;176(13):4092-103.

  21. McGlynn P, Lloyd RG. Genome stability and the processing of damaged replication forks by RecG. Trends Genet. 2002;18(8):413-9.

  22. Lopez CR, Yang S, Deibler RW, Ray SA, Pennington JM, Digate RJ, et al. A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol Microbiol. 2005;58(1):80-101.

  23. Grove JI, Harris L, Buckman C, Lloyd RG. DNA double strand break repair and crossing over mediated by RuvABC resolvase and RecG translocase. DNA Repair. 2008;7(9):1517-30.

  24. Lovett ST, Hurley RL, Sutera VA Jr., Aubuchon RH, Lebedeva MA. Crossing over between regions of limited homology in Escherichia coli. RecA-dependent and RecA-independent pathways. Genetics. 2002;160(3):851-9.

  25. Dutra BE, Sutera VA, Jr., Lovett ST. RecA- independent recombination is efficient but limited by exonucleases. Proc Natl Acad Sci USA. 2007;104(1):216-21.

  26. Swingle B, Markel E, Costantino N, Bubunenko MG, Cartinhour S, Court DL. Oligonucleotide recombination in Gram-negative bacteria. Mol Microbiol. 2010;75(1):138-48.

  27. Poteete AR. Expansion of a chromosomal repeat in Escherichia coli: roles of replication, repair, and recombination functions. BMC Mol Biol. 2009;10:14.

  28. Ivankovic S, Dermic D. DNA end resection controls the balance between homologous and illegitimate recombination in Escherichia coli. PloS One. 2012;7(6):e39030.

  29. Cardenas PP, Carrasco B, Defeu Soufo C, Cesar CE, Herr K, Kaufenstein M, et al. RecX facilitates homologous recombination by modulating RecA activities. PLoS Genet. 2012;8(12):e1003126.

  30. Sakai A, Cox MM. RecFOR and RecOR as distinct RecA loading pathways. J Biol Chem. 2009;284(5):3264-72.

  31. Morimatsu K, Wu Y, Kowalczykowski SC. RecFOR proteins target RecA protein to a DNA gap with either DNA or RNA at the 5’ terminus: implication for repair of stalled replication forks. J Biol Chem. 2012;287(42):35621-30.

  32. Lehmann AR, Niimi A, Ogi T, Brown S, Sabbioneda S, Wing JF, et al. Translesion synthesis: Y-family polymerases and the polymerase switch. DNA repair. 2007;6(7):891-9.

  33. Shukla AK, Roy KB. Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA. Biol Chem. 2006;387(3):251-6.

  34. Seigneur M, Ehrlich SD, Michel B. RuvABC-dependent double-strand breaks in dnaBts mutants require recA. Mol Microbiol. 2000;38(3):565-74.

  35. Al-Hadid Q, Ona K, Courcelle CT, Courcelle J. RecA433 cells are defective in recF-mediated processing of disrupted replication forks but retain recBCD-mediated functions. Mutat Res. 2008;645(1- 2):19-26.

  36. Belle JJ, Casey A, Courcelle CT, Courcelle J. Inactivation of the DnaB helicase leads to the collapse and degradation of the replication fork: a comparison to UV-induced arrest. J Bacteriol. 2007;189(15):5452-62.

  37. Khan SR, Kuzminov A. Replication forks stalled at ultraviolet lesions are rescued via RecA and RuvABC protein-catalyzed disintegration in Escherichia coli. J Biol Chem. 2012;287(9):6250-65.

  38. Ishioka K, Fukuoh A, Iwasaki H, Nakata A, Shinagawa H. Abortive recombination in Escherichia coli ruv mutants blocks chromosome partitioning. Genes Cells. 1998;3(4):209-20.

  39. Jaktaji RP. Using transposon mutagenesis to find an alternative resolvase in an Escherichia coli cells lacking RuvABC. Pakistan J Biol Sci. 2009;12(6):534-7.

  40. Jeiranian HA, Courcelle CT, Courcelle J. Inefficient replication reduces RecA-mediated repair of UV-damaged plasmids introduced into competent Escherichia coli. Plasmid. 2012;68(2):113-24.

  41. Courcelle J, Carswell-Crumpton C, Hanawalt PC. recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli. Proc Natl Acad Sci USA. 1997;94(8):3714-9.

  42. Sutherland BM, Bennett PV, Sidorkina O, Laval J. Clustered damages and total lesions induced in DNA by ionizing radiation: oxidized bases and strand breaks. Biochemistry. 2000;39(27):8026-31.

  43. Bichara M, Meier M, Wagner J, Cordonnier A, Lambert IB. Postreplication repair mechanisms in the presence of DNA adducts in Escherichia coli. Mutat Res. 2011;727(3):104-22.

  44. Reams AB, Kofoid E, Kugelberg E, Roth JR. Multiple pathways of duplication formation with and without recombination (RecA) in Salmonella enterica. Genetics. 2012;192(2):397-415.

  45. Killoran MP, Keck JL. Sit down, relax and unwind: structural insights into RecQ helicase mechanisms. Nucleic Acids Res. 2006;34(15):4098-105.

  46. Hua X, Huang L, Tian B, Hua Y. Involvement of recQ in the ultraviolet damage repair pathway in Deinococcus radiodurans. Mutat Res. 2008;641(1-2):48-53.

  47. Kovacic L, Paulic N, Leonardi A, Hodnik V, Anderluh G, Podlesek Z, et al. Structural insight into LexA-RecA* interaction. Nucleic Acids Res. 2013;41(21):9901-10.

  48. Hishida T, Han YW, Shibata T, Kubota Y, Ishino Y, Iwasaki H, et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004;18(15):1886-97.

  49. Nakayama H, Nakayama K, Nakayama R, Irino N, Nakayama Y, Hanawalt PC. Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway. Mol Gen Genet. 1984;195(3):474-80.

  50. Thoms B, Borchers I, Wackernagel W. Effects of single-strand DNases ExoI, RecJ, ExoVII, and SbcCD on homologous recombination of recBCD+ strains of Escherichia coli and roles of SbcB15 and XonA2 ExoI mutant enzymes. J Bacteriol. 2008;190(1):179-92.

  51. Feng WY, Hays JB. DNA structures generated during recombination initiated by mismatch repair of UV-irradiated nonreplicating phage DNA in Escherichia coli: requirements for helicase, exonucleases, and RecF and RecBCD functions. Genetics. 1995;140(4):1175-86.

  52. Ivancic-Bace I, Vlasic I, Salaj-Smic E, Brcic- Kostic K. Genetic evidence for the requirement of RecA loading activity in SOS induction after UV irradiation in Escherichia coli. J Bacteriol. 2006;188(14):5024-32.

  53. Jockovich ME, Myers RS. Nuclease activity is essential for RecBCD recombination in Escherichia coli. Mol Microbiol. 2001;41(4): 949-62.

  54. Casper AM, Rosen DM, Rajula KD. Sites of genetic instability in mitosis and cancer. Ann N Y Acad Sci. 2012;1267:24-30.

  55. Evers B, Helleday T, Jonkers J. Targeting homologous recombination repair defects in cancer. Trends Pharmacol Sci. 2010;31(8):372-80.

  56. Helleday T. Homologous recombination in cancer development, treatment and development of drug resistance. Carcinogenesis. 2010;31(6):955-60.

  57. Bouwman P, Jonkers J. The effects of deregulated DNA damage signaling on cancer chemotherapy response and resistance. Nat Rev Cancer. 2012;12(9):587-98.




2020     |     www.medigraphic.com