Bautista GCR, Pedraza AG, Rosenstein AY, Ontiveros FJ

Idioma: Español/Inglés
Referencias bibliográficas: 45
Paginas: 247-259
Archivo PDF: 121.95 Kb.

RESUMEN

Se determinaron las fluctuaciones de linfocitos T CD2+, CD4+ y CD8+, anticuerpos IgG anti-Anaplasma (Acs) e interferon gamma (IFN-g) en el suero y en cultivo de sangre completa (CSC) de bovinos infectados con el mismo aislado de Anaplasma marginale. Se utilizaron siete bovinos de 12 a 14 meses de edad. El día 0 fueron inoculados cuatro de ellos (grupo I) por vía intravenosa con 1 × 108 eritrocitos parasitados (EP), con el aislado Morelos de A. marginale/animal, y con 2 × 108 EP el día 60; mientras que los otros tres bovinos (grupo T) no fueron infectados. Se colectaron muestras de sangre periférica y suero de todos los animales, para evaluar el volumen celular aglomerado (VCA), porcentaje de eritrocitos parasitados (PEP), linfocitos T CD2+, CD4+ y CD8+ por citofluorometría y Acs e IFN-g por ELISA. En el grupo I, el PEP alcanzó un promedio máximo de 5.2% el día 31 posinfección (pi), mientras que el VCA disminuyó hasta un promedio de 6% el día 46 pi. En la reinfección (ri), el PEP fue de 0 y el VCA fue normal. Los valores de CD2+ fueron similares en los dos grupos durante el estudio; en cambio, los de CD4+ y CD8+ en el grupo I disminuyeron significativamente (P ‹ 0.05) en el día 45 pi, para después alcanzar valores similares a los del grupo T. Se observó un aumento (P ‹ 0.05) de la intensidad de fluorescencia (IF) en los linfocitos CD2+ y CD8+ del grupo I después de la ri (días 62 y 83) con respecto a la IF determinada en los linfocitos del grupo T. Los niveles de Acs de los bovinos infectados aumentaron por encima de los valores basales y de los de los animales testigo, a partir del día 51 pi. Asimismo, los niveles de IFN-g, tanto en el suero como en el sobrenadante de CSC de los bovinos infectados, fueron significativamente más altos (P ‹ 0.05) que los de los animales no infectados, particularmente en la ri.

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