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2012, Número 3

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Dermatología Cosmética, Médica y Quirúrgica 2012; 10 (3)


ELISA y sus aplicaciones en dermatología

Ríos YJM, Mercadillo PP, Yuil RE, Ríos CM

Idioma: Español
Referencias bibliográficas: 72
Paginas: 212-222
Archivo PDF: 373.99 Kb.


RESUMEN

ELISA es una técnica que permite la detección de antígenos o anticuerpos específicos en una muestra de interés, mediante una combinación de principios inmunológicos y enzimáticos. Toda prueba de ELISA tiene en común los siguientes componentes: un antígeno o un anticuerpo específico marcado con una enzima, un soporte, un sustrato que será transformado por la enzima en un producto detectable y un sistema para detectarlo. Los principales tipos de ELISA son el directo, el indirecto y el de sándwich. Las aplicaciones en dermatología son abundantes, y es utilizado principalmente para el diagnóstico, con alta sensibilidad y especificidad, de dermatosis infecciosas, ampollares, vasculitis, enfermedades del tejido conectivo y para la medición de citocinas que están relacionadas con la patogénesis de múltiples enfermedades con repercusión cutánea. Esta técnica también tiene aplicaciones crecientes en la investigación dermatológica, por lo que todo dermatólogo debe conocer los principios básicos de esta prueba.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Crowther JR. “Enzyme-linked Immunosorbent Assay (ELISA)”. En: R Rapley, JM Walker (comps). Molecular Biomethods Handbook. Totowa, Nueva Jersey, 1998: 595-617.

  2. Cruse JM, Lewis RE. Atlas of Immunology. 3a ed.; Boca Raton: CRC Press, 2010: 841.

  3. Abbas AK, Lichtman AH, Pillai S. Inmunología celular y molecular. 6a ed.; Barcelona: Elsevier, 2008: 525-527.

  4. Cedeno-Laurent F, Gómez-Flores M, Méndez N, Ancer-Rodríguez J, Bryant JL, Gaspari AA et al. “New insights into HIV-1-primary skin disorders”. J Int AIDS Soc. 2011; 14: 5.

  5. Indrati AR, Van Crevel R, Parwati I, Tjandrawati A, Noormartany, Kurmalawati J. “Screening and diagnosis of HIV-infection in Indonesia: One, two or three tests? Acta Med Indones. Julio, 2009; 41 Suppl 1: 28-32.

  6. Ríos JM, Ríos M. “El virus del papiloma humano y su relación con el cáncer cutáneo no melanoma”. Rev Méd Cient. 2010; 23(2): 10-21.

  7. Karem KL, Poon AC, Bierl C, Nisenbaum R, Unger E. “Optimization of a human papillomavirus-specific enzyme-linked immunosorbent assay”. Clin Diagn Lab Immunol. Mayo, 2002; 9(3): 577-582.

  8. Delany-Moretlwe S, Jentsch U, Weiss H, Moyes J, Ashley-Morrow R, Stevens W et al. “Comparison of focus Herpes Select and Kalon HSV- 2 gG2 ELISA serological assays to detect herpes simplex virus type 2 antibodies in a South African population”. Sex Transm Infect. Febrero, 2010; 86(1): 46-50.

  9. Guzmán MG, Jaenisch T, Gaczkowski R, Ty Hang VT, Sekaran SD, Kroeger A et al. “Multi-country evaluation of the sensitivity and specificity of two commercially-available NS1 ELISA assays for dengue diagnosis”. PLoS Negl Trop Dis. Agosto 31, 2010; 4(8). pii: e811.

  10. Roodbari F, Roustai MH, Mostafaie A, Soleimanjdahi H, Foroshani RS, Sabahi F. “Development of an enzyme-linked immunosorbent assay for immunoglobulin M antibodies against measles virus”. Clin Diagn Lab Immunol. Mayo, 2003; 10(3): 439-442.

  11. Mossong J, Putz L, Schneider F. “Seroprevalence of measles, mumps and rubella antibodies in Luxembourg: Results from a national crosssectional study”. Epidemiol Infect. Enero, 2004; 132(1): 11-18.

  12. Scott LA, Stone MS. “Viral exanthems”. Dermatol Online J. Agosto, 2003; 9(3): 4.

  13. Mueller NH, Gilden DH, Cohrs RJ, Mahalingam R, Nagel MA. “Varicella zoster virus infection: Clinical features, molecular pathogenesis of disease, and latency”. Neurol Clin. Agosto, 2008; 26(3): 675-697, viii.

  14. Sauerbrei A, Wutzler P. “Serological detection of varicella-zoster virusspecific immunoglobulin G by an enzyme-linked immunosorbent assay using glycoprotein antigen”. J Clin Microbiol. Septiembre, 2006 ; 44(9): 3094-3097.

  15. Cruz AR, Pillay A, Zuluaga AV, Ramírez LG, Duque JE, Aristizabal GE et al. “Secondary syphilis in cali, Colombia: New concepts in disease pathogenesis”. PLoS Negl Trop Dis. Mayo 18, 2010; 4(5): 690.

  16. Shimanskaya I, Zhurauskaya L, Pankratov O, Unemo M, Ballard RC, Domeika M. “Evaluation of three serological tests manufactured in Belarus for the diagnosis of syphilis”. Acta Derm Venereol. 2011; 91: 299-302.

  17. Chen CY, Mertz KJ, Spinola SM, Morse SA. “Comparison of enzyme immunoassays for antibodies to Haemophilus ducreyi in a community outbreak of chancroid in the United States”. J Infect Dis. Enero, 1997; 175(6): 1390-1395.

  18. Mabey D, Peeling RW. “Lymphogranuloma venereum”. Sex Transm Infect. Abril, 2002; 78(2): 90-92.

  19. Wills GS, Horner PJ, Reynolds R, Johnson AM, Muir DA, Brown DW et al. “Pgp3 antibody enzyme-linked immunosorbent assay, a sensitive and specific assay for seroepidemiological analysis of Chlamydia trachomatis infection”. Clin Vaccine Immunol. Enero, 2009; 16(6): 835-843.

  20. Burbelo PD, Issa AT, Ching KH, Cohen JI, Iadarola MJ, Marques A et al. “Rapid, simple, quantitative, and highly sensitive antibody detection for lyme disease”. Clin Vaccine Immunol. Enero, 2010; 17(6): 904-909.

  21. Hoey JG, Valois-Cruz F, Goldenberg H, Voskoboynik Y, Pfiffner J, Tilton RC et al. “Development of an immunoglobulin M capturebased enzyme-linked immunosorbent assay for diagnosis of acute infections with Bartonella henselae”. Clin Vaccine Immunol. Febrero, 2009; 16(2): 282-284.

  22. Bratton RL, Corey R. “Tick-borne disease”. Am Fam Physician. Enero 15, 2005; 71(12): 2323-2330.

  23. Schmitt P, Splettstösser W, Porsch-Ozcürümez M, Finke EJ, Grunow R et al. “A novel screening ELISA and a confirmatory Western blot useful for diagnosis and epidemiological studies of tularemia”. Epidemiol Infect. Agosto, 2005; 133(4): 759-766.

  24. Eliasson H, Olcén P, Sjöstedt A, Jurstrand M, Bäck E, Andersson S. “Kinetics of the immune response associated with tularemia: Comparison of an enzyme-linked immunosorbent assay, a tube agglutination test, and a novel whole-blood lymphocyte stimulation test”. Clin Vaccine Immunol. Agosto, 2008; 15(8): 1238-1243.

  25. Kai M, Nguyen Phuc NH, Hoang Thi TH, Nguyen AH, Fukutomi Y, Maeda Y. “Serological diagnosis of leprosy in patients in Vietnam by enzyme-linked immunosorbent assay with Mycobacterium leprae-derived major membrane protein II”. Clin Vaccine Immunol. Diciembre, 2008; 15(12): 1755-1759.

  26. Hatta M, Makino M, Ratnawati, Mashudi,Yadi, Sabir M et al. “Detection of serum antibodies to M. leprae major membrane protein-II in leprosy patients from Indonesia”. Lepr Rev. Diciembre, 2009; 80(4): 402-409.

  27. Steingart KR, Henry M, Laal S, Hopewell PC, Ramsay A, Menzies D et al. “A systematic review of commercial serological antibody detection tests for the diagnosis of extrapulmonary tuberculosis”. Thorax. Octubre, 2007; 62(10): 911-918.

  28. Oberto-Perdigón L, Romero H, Pérez-Blanco M, Apitz-Castro R et al. “An ELISA test for the study of the therapeutic evolution of chromoblastomycosis by Cladophialophora carrionii in the endemic area of Falcon State, Venezuela”. Rev Iberoam Micol. 2005; 22: 39-43.

  29. Vidal MS, De Castro LG, Cavalecate SC, Lacaz Cda S et al. “Immunoprecipitation techniques and Elisa in the detection of anti-Fonsecaea pedrosoi antibodies in chromoblastomycosis”. Rev Inst Med Trop Sao Paulo. Noviembre-diciembre, 2003; 45(6): 315-318.

  30. Almeida-Paes R, Pimenta MA, Pizzini CV, Monteiro PC, Peralta JM, Nosanchuk JD et al. “Use of mycelial-phase Sporothrix schenckii exoantigens in an enzyme-linked immunosorbent assay for diagnosis of sporotrichosis by antibody detection”. Clin Vaccine Immunol. Marzo, 2007; 14(3): 244-249.

  31. Almeida-Paes R, Pimenta MA, Monteiro PC, Nosanchuk JD, Zancopé- Oliveira RM. “Immunoglobulins G, M, and A against Sporothrix schenckii exoantigens in patients with sporotrichosis before and during treatment with itraconazole”. Clin Vaccine Immunol. Septiembre, 2007; 14(9): 1149-1157.

  32. Müller CS, Schmaltz R, Vogt T. “Secondary cutaneous cryptococcosis mimicking a malignant ulcerated tumor of the facial skin in an immunosuppressed patient”. Eur J Dermatol. Septiembre-octubre, 2010; 20(5): 657-658.

  33. Illnait MT, Vilaseca JC, Fernández CM, Martínez GF et al. “Enzymelinked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen”. Mem Inst Oswaldo Cruz. Febrero, 2001; 96(2): 241-245.

  34. Santangelo RT, Chen SC, Sorrell TC, Wright LC. “Detection of antibodies to phospholipase B in patients infected with Cryptococcus neoformans by enzyme-linked immunosorbent assay (ELISA)”. Med Mycol. Enero, 2005; 43(4): 335-341.

  35. Albuquerque CF, Da Silva SH, Camargo ZP. “Improvement of the specificity of an enzyme-linked immunosorbent assay for diagnosis of paracoccidioidomycosis”. J Clin Microbiol. Abril, 2005; 43(4): 1944-1946.

  36. Da Silva SH, Grosso D de M, Lopes JD, Colombo AL, Blotta MH, Queiroz-Telles F et al. “Detection of Paracoccidioides brasiliensis gp70 circulating antigen and follow-up of patients undergoing antimycotic therapy”. J Clin Microbiol. Octubre, 2004; 42(10): 4480-4486.

  37. Bellissimo-Rodrigues F, Vitali LH, Martinez R. “Serological diagnosis of paracoccidioidomycosis in HIV-coinfected patients”. Mem Inst Oswaldo Cruz. Noviembre, 2010; 105(7): 904-907.

  38. Scheel CM, Samayoa B, Herrera A, Lindsley MD, Benjamin L, Reed Y et al. “Development and evaluation of an enzyme-linked immunosorbent assay to detect Histoplasma capsulatum antigenuria in immunocompromised patients”. Clin Vaccine Immunol. Enero, 2009; 16(6):852-858.

  39. Guimarães AJ, Pizzini CV, De Matos Guedes HL, Albuquerque PC, Peralta JM, Hamilton AJ et al. “ELISA for early diagnosis of histoplasmosis”. J Med Microbiol. Enero, 2004; 53(Pt 6): 509-514.

  40. Blair JE, Coakley B, Santelli AC, Hentz JG, Wengenack NL. “Serological testing for symptomatic coccidioidomycosis in immunocompetent and immunosuppressed hosts”. Mycopathologia. Noviembre, 2006; 162(5): 317-324.

  41. Martins TB, Jaskowski TD, Mouritsen CL, Hill HR et al. “Comparison of commercially available enzyme immunoassay with traditional serological tests for detection of antibodies to Coccidioides immitis”. J Clin Microbiol. Abril, 1995; 33(4): 940-943.

  42. Ríos JM, Sousa O. “Inmunología en la infección por Leishmania: conceptos actuales”. Rev Méd Cient. 2010; 23: 11-23.

  43. Ryan JR, Smithyman AM, Rajasekariah GH, Hochberg L, Stiteler JM, Martin SK. “Enzyme-linked immunosorbent assay based on soluble promastigote antigen detects immunoglobulin M (IgM) and IgG antibodies in sera from cases of visceral and cutaneous leishmaniasis”. J Clin Microbiol. Marzo, 2002; 40(3): 1037-1043.

  44. Ríos JM, Yuil de Ríos E. “Métodos diagnósticos parasitológicos, inmunológicos, histopatológicos y moleculares de leishmaniasis cutánea”. Rev Méd Cient. 2010; 23(2): 22-37.

  45. Silvestre R, Santarém N, Teixeira L, Cunha J, Schallig H, Cordeiro-da-Silva A. “Evaluation of Leishmania species reactivity in human serologic diagnosis of leishmaniasis”. Am J Trop Med Hyg. Agosto, 2009; 81(2): 202-208.

  46. Bumb RA, Mehta RD. “Amoebiasis cutis in HIV positive patient”. Indian J Dermatol Venereol Leprol. Mayo-junio, 2006; 72(3): 224-226.

  47. Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J et al. “Laboratory diagnostic techniques for Entamoeba species”. Clin Microbiol Rev. Julio, 2007; 20(3): 511-532.

  48. Van Doorn HR, Hofwegen H, Koelewijn R, Gilis H, Peek R, Wetsteyn JC et al. “Use of rapid dipstick and latex agglutination tests and enzymelinked immunosorbent assay for serodiagnosis of amebic liver abscess, amebic colitis, and Entamoeba histolytica cyst passage”. J Clin Microbiol. Septiembre, 2005; 43(9): 4801-4806.

  49. Babalola OE. “Ocular onchocerciasis: Current management and future prospects”. Clin Ophthalmol. 2011; 5: 1479-1491.

  50. Gómez-Priego A, Mendoza R, De la Rosa JL. “Prevalence of antibodies to Onchocerca volvulus in residents of Oaxaca, Mexico, treated for 10 years with ivermectin”. Clin Diagn Lab Immunol. Enero, 2005; 12(1): 40-43.

  51. Amagai M. “Autoimmune and infectious skin diseases that target desmogleins”. Proc Jpn Acad Ser B Phys Biol Sci. 2010; 86(5): 524-537.

  52. Wiwanitkit V. “A systematic metanalysis on diagnostic value of Dsg3 ELISA for pemphigus vulgaris”. Indian J Dermatol. 2009; 54(2): 192.

  53. Khandpur S, Sharma VK, Sharma A, Pathria G, Satyam A. ”Comparison of enzyme-linked immunosorbent assay test with immunoblot assay in the diagnosis of pemphigus in Indian patients”. Indian J Dermatol Venereol Leprol. Enero-febrero, 2010; 76(1): 27-32.

  54. Khandpur S, Verma P. “Bullous pemphigoid”. Indian J Dermatol Venereol Leprol. 2011; 77: 450-455.

  55. Csorba K, Schmidt S, Florea F, Ishii N, Hashimoto T, Hertl M et al. “Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases”. Orphanet J Rare Dis. Mayo, 2011; 6: 31.

  56. Lu PD, Ralston J, Kamino H, Stein JA. “Pemphigoid gestationis”. Dermatol Online J. Noviembre 15, 2010; 16(11): 10.

  57. Di Zenzo G, Calabresi V, Grosso F, Caproni M, Ruffelli M, Zambruno G. “The intracellular and extracellular domains of BP180 antigen comprise novel epitopes targeted by pemphigoid gestationis autoantibodies”. J Invest Dermatol. Abril, 2007; 127(4): 864-873.

  58. Barnadas MA, Rubiales MV, González MJ, Puig L, García P, Baselga E et al. “Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence testing in a bullous pemphigoid and pemphigoid gestationis”. Int J Dermatol. Diciembre, 2008; 47(12): 1245-1249.

  59. Herrero-González JE. “Guía clínica de diagnóstico y tratamiento de la dermatitis herpetiforme”. Actas Dermosifiliogr. 2010; 101(10): 820-826.

  60. Sárdy M, Kárpáti S, Péterfy F, Rásky K, Tomsits E, Zágoni T et al. “Comparison of a tissue transglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy”. Z Gastroenterol. Mayo, 2000; 38(5): 357-364.

  61. Chen M, Doostan A, Bandyopadhyay P, Remington J, Wang X, Hou Y et al. “The cartilage matrix protein subdomain of type VII collagen is pathogenic for epidermolysis bullosa acquisita”. Am J Pathol. Enero, 2007; 170(6): 2009-2018.

  62. Saleh MA, Ishii K, Kim YJ, Murakami A, Ishii N, Hashimoto T et al. “Development of NC1 and NC2 domains of type VII collagen ELISA for the diagnosis and analysis of the time course of epidermolysis bullosa acquisita patients”. J Dermatol Sci. Enero, 2011; 62(3): 169-175.

  63. Trevisin M, Pollock W, Dimech W, Melny J, Paspaliaris B, Gillis D et al. “Antigen-specific ANCA ELISAs have different sensitivities for active and treated vasculitis and for nonvasculitic disease”. Am J Clin Pathol. Enero, 2008; 129(1): 42-53.

  64. Csernok E, Holle J, Hellmich B, Willem J, Tervaert C, Kallenberg CG et al. “Evaluation of capture ELISA for detection of antineutrophil cytoplasmic antibodies directed against proteinase 3 in Wegener’s granulomatosis: First results from a multicentre study”. Rheumatology. Febrero, 2004; 43(2): 174-180.

  65. Satoh M, Vázquez-del Mercado M, Chan EK et al. “Clinical interpretation of antinuclear antibody tests in systemic rheumatic diseases”. Mod Rheumatol. 2009; 19(3): 219-228.

  66. Akbarali Y, Matousek-Ronck J, Hunt L, Staudt L, Reichlin M, Guthridge JM et al. “Fine specificity mapping of autoantigens targeted by anticentromere autoantibodies”. J Autoimmun. 2006; 27(4): 272-280.

  67. Richards TJ, Eggebeen A, Gibson K, Yousem S, Fuhrman C, Gochuico BR et al. “Characterization and peripheral blood biomarker assessment of Jo-1 antibody-positive intersticial lung disease”. Arthritis Rheum. Julio, 2009; 60(7): 2183-2192.

  68. Stone KB, Oddis CV, Fertig N, Katsumata Y, Lucas M, Vogt M et al. “Anti- Jo-1 antibody levels correlate with disease activity in idiopathic inflammatory myopathy”. Arthritis Rheum. Septiembre, 2007; 56(9): 3125-3131.

  69. Tarzi MD, Hickey A, Förster T, Mohammadi M, Longhurst HJ et al. ”An evaluation of tests used for the diagnosis and monitoring of C1 inhibitor deficiency: Normal serum C4 does not exclude hereditary angio-oedema”. Clin Exp Immunol. Septiembre, 2007; 149(3): 513-516.

  70. Ríos JM, Ríos M. “Inmunogenética de la psoriasis y de la artritis psoriásica”. Rev Méd Cient. 2010; 23(1): 32-40.

  71. Ríos JM, Adames M, Yuil de Ríos E, Guevara Y. “Correlación entre severidad clínica, TNF-α y resistencia a la insulina en la psoriasis”. Dermatología CMQ. 2011; 9(4): 255-262.

  72. Arican O, Aral M, Sasmaz S, Ciragil P et al. “Serum levels of TNF-alpha, IFN-gamma, IL-6, IL-8, IL-12, IL-17, and IL-18 in patients with active psoriasis and correlation with disease severity”. Mediators Inflamm. Octubre 24, 2005; 2005(5): 273-279.




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