Vargas-González A, Prado-Zayago E, León-Olea M, Guarner-Lans V, Cano MA

Idioma: Español
Referencias bibliográficas: 35
Paginas: 21-29
Archivo PDF: 208.03 Kb.

RESUMEN

Se realizó resección ventricular en el corazón de Ambystoma mexicanum, se evaluó si la restitución del tejido resulta de hipertrofia o de hiperplasia. Por medio de una tinción tricrómica se encontró que 5 días después del daño en el espacio de la resección se encontró un coágulo rodeado de fibras de colágena (83 ± 6%), músculo (10 ± 3%) y zonas sin tejido (7 ± 2%). Una proporción de 50 ± 4 y 90 ± 2% correspondió a tejido muscular 10 y 30 días después de la lesión. La tinción con bis-Benzimida indicó que en la zona lesionada hay proliferación celular. En tanto que la inmunohistoquímica doble para actina sarcomérica a y antígeno nuclear de proliferación celular mostró que el tejido que restituyó el espacio se produjo por proliferación de cardiomiocitos con un valor máximo de 68%, 5 días después de la lesión. Nuestros resultados indican que el miocardio de A. mexicanum recupera su estructura mediante la hiperplasia de cardiomiocitos y sugieren que su capacidad regenerativa es mayor que la informada para mamíferos adultos (1%), y para otros vertebrados no mamíferos (32%). Esto hace de A. mexicanum un modelo idóneo para estudiar los mecanismos reguladores de la regeneración miocárdica per se, en vertebrados adultos in vivo.

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