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2013, Número 1

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Biotecnol Apl 2013; 30 (1)


Tipificación molecular de Streptococcus suis aislado de cerdos en Cuba

Espinosa I, Báez M, Corona B, Chong D, Lobo E, Martínez S

Idioma: Ingles.
Referencias bibliográficas: 37
Paginas: 39-44
Archivo PDF: 181.73 Kb.


RESUMEN

Streptococcus suis es una bacteria común en el tracto respiratorio de los cerdos; sin embargo las infecciones causadas por cepas virulentas se consideran un problema en la industria porcina. Estas cepas se pueden identificar por la diferenciación de los serotipos capsulares con antisueros específicos o por sus correspondientes tipos cps, mediante ensayos genotípicos. También se pueden reconocer mediante la detección de factores asociados con la virulencia, como el factor extracelular, la proteína liberada por muramidasa y la hemolisina suilisina. Antes de esta investigación no había datos sobre la identifi cación serológica o molecular de los tipos capsulares de S. suis procedentes de cerdos en Cuba. El objetivo de esta investigación fue la detección de los tipos capsulares cps2, cps7 y cps9, y de los tres genes asociados con la virulencia, mediante ensayos de reacción en cadena de la polimerasa. De 31 aislamientos, 21 se clasificaron con el genotipo capsular cps2, y 4 con el cps9. El genotipo capsular cps7 no se detectó y 6 aislados no se correspondieron con ninguno de los serotipos analizados. Considerando la presencia de los genes sly, epf y mrp, se identificaron seis genotipos en los aislados cps2 y cps9, y 3 en los aislados cuyo genotipo cps no se identificó. Los aislados cps2 se extrajeron de cerdos de 6 a 12 con neumonía y de 14 a 17 semanas con infección sistémica; mientras que los cps9 estaban asociados exclusivamente con neumonía.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Berthelot-Herault F, Gottschalk M, Morvan H, Kobisch M. Dilemma of virulence of Streptococcus suis: Canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs. Can J Vet Res. 2005;69(3):236-40.

  2. Kim D, Han K, Oh Y, Kim CH, Kang I, Lee J, et al. Distribution of capsular serotypes and virulence markers of Streptococcus suis isolated from pigs with polyserositis in Korea. Can J Vet Res. 2010;74(4):314-6.

  3. Baums CG, Verkuhlen GJ, Rehm T, Silva LM, Beyerbach M, Pohlmeyer K, et al. Prevalence of Streptococcus suis genotypes in wild boars of Northwestern Germany. Appl Environ Microbiol. 2007;73(3):711-7.

  4. Baums CG, Valentin-Weigand P. Surface- associated and secreted factors of Streptococcus suis in epidemiology, pathogenesis and vaccine development. Anim Health Res Rev. 2009;10(1):65-83.

  5. King SJ, Heath PJ, Luque I, Tarradas C, Dowson CG, Whatmore AM. Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different diseases of pigs and characterization of the genetic basis of suilysin absence. Infect Immun. 2001;69(12):7572-82.

  6. Gottschalk M, Segura M. The pathogenesis of the meningitis caused by Streptococcus suis: the unresolved questions. Vet Microbiol. 2000;76(3):259-72.

  7. Tang J, Wang C, Feng Y, Yang W, Song H, Chen Z, et al. Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2. PLoS Med. 2006;3(5):e151.

  8. Wertheim HF, Nghia HD, Taylor W, Schultsz C. Streptococcus suis: an emerging human pathogen. Clin Infect Dis. 2009; 48(5):617-25.

  9. Heath PJ, Hunt BW. Streptococcus suis serotypes 3 to 28 associated with disease in pigs. Vet Rec. 2001;148(7):207-8.

  10. Hill JE, Gottschalk M, Brousseau R, Harel J, Hemmingsen SM, Goh SH. Biochemical analysis, cpn60 and 16S rDNA sequence data indicate that Streptococcus suis serotypes 32 and 34, isolated from pigs, are Streptococcus orisratti. Vet Microbiol. 2005;107(1-2):63-9.

  11. Smith HE, van Bruijnsvoort L, Buijs H, Wisselink HJ, Smits MA. Rapid PCR test for Streptococcus suis serotype 7. FEMS Microbiol Lett. 1999;178(2):265-70.

  12. Smith HE, Veenbergen V, van der Velde J, Damman M, Wisselink HJ, Smits MA. The cps genes of Streptococcus suis serotypes 1, 2, and 9: development of rapid serotype-specifi c PCR assays. J Clin Microbiol. 1999;37(10):3146-52.

  13. Marois C, Le Devendec L, Gottschalk M, Kobisch M. Detection and molecular typing of Streptococcus suis in tonsils from live pigs in France. Can J Vet Res. 2007; 71(1):14-22.

  14. Silva LM, Baums CG, Rehm T, Wisselink HJ, Goethe R, Valentin-Weigand P. Virulence-associated gene profi ling of Streptococcus suis isolates by PCR. Vet Microbiol. 2006;115(1-3):117-27.

  15. Beineke A, Bennecke K, Neis C, Schroder C, Waldmann KH, Baumgartner W, et al. Comparative evaluation of virulence and pathology of Streptococcus suis serotypes 2 and 9 in experimentally infected growers. Vet Microbiol. 2008;128(3-4): 423-30.

  16. Del’Arco AE, Santos JL, Bevilacquia PD, Faria JE, Guimaraes WV. Swine infection by Streptococcus suis: a retrospective study. Arq Bras Med Vet Zootec. 2008; 60(4):878-83.

  17. Wisselink HJ, Smith HE, Stockhofe- Zurwieden N, Peperkamp K, Vecht U. Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet Microbiol. 2000;74(3):237-48.

  18. Muñiz M, Valdivia MP, Heredia J, López O, Arias T, Mederos CM, et al. Gestión tecnológica en la producción porcina cubana. In: VI Encuentro regional sobre nutrición y producción de especies monogástricas. La Habana: Asociación Cubana de producción animal; 2001. Available from: http:// www.sian.info.ve/porcinos/publicaciones/ viencuent/marisol.htm

  19. Berthelot-Herault F, Morvan H, Keribin AM, Gottschalk M, Kobisch M. Production of muraminidase-released protein (MRP), extracellular factor (EF) and suilysin by fi eld isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France. Vet Res. 2000;31(5):473-9.

  20. Luther DA, Almeida RA, Oliver SP. Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene (sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations. Vet Microbiol. 2008;128(3-4):304-12.

  21. Staats JJ, Plattner BL, Stewart GC, Changappa MM. Presence of the Streptococcus suis suilysin gene and expression of MRP and EF correlates with high virulence in Streptococcus suis type 2 isolates. Vet Microbiol. 1999;70(3-4):201-11.

  22. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731-9.

  23. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.

  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol. 1980; 16(2):111-20.

  25. Felsenstein J. Confi dence limits on phylogenies: An approach using the bootstrap. Evolution. 1985; 39:783-91.

  26. MacInnes JI, Desrosiers R. Agents of the “suis-ide diseases” of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis. Can J Vet Res. 1999; 63(2):83-9.

  27. Messier S, Lacouture S, Gottschalk M. Distribution of Streptococcus suis capsular types from 2001 to 2007. Can Vet J. 2008; 49(5):461-2.

  28. Dee SA, Corey MM. The survival of Streptococcus suis on farm and veterinary equipment. Swine Health Prod. 1993: 1(1):17-20.

  29. Luque I, Blume V, Borge C, Vela AI, Perea JA, Marquez JM, et al. Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm. Vet J. 2010;186(3):396-8.

  30. Su Y, Yao W, Perez-Gutierrez ON, Smidt H, Zhu WY. Changes in abundance of Lactobacillus spp. and Streptococcus suis in the stomach, jejunum and ileum of piglets after weaning. FEMS Microbiol Ecol. 2008;66(3):546-55.

  31. Princivalli MS, Palmieri C, Magi G, Vignaroli C, Manzin A, Camporese A, et al. Genetic diversity of Streptococcus suis clinical isolates from pigs and humans in Italy (2003- 2007). Euro Surveill. 2009;14(33): pii: 19310. Available from: http://www.eurosurveillance. org/ViewArticle.aspx?ArticleId=19310

  32. Wei Z, Li R, Zhang A, He H, Hua Y, Xia J, et al. Characterization of Streptococcus suis isolates from the diseased pigs in China between 2003 and 2007. Vet Microbiol. 2009;137(1-2):196-201.

  33. Tarradas C, Borge C, Arenas A, Maldonado A, Astorga R, Miranda A, et al. Suilysin production by Streptococcus suis strains isolated from diseased and healthy carrier pigs in Spain. Vet Rec. 2001; 148(6):183-4.

  34. Smith HE, Vecht U, Wisselink HJ, Stockhofe- Zurwieden N, Biermann Y, Smits MA. Mutants of Streptococcus suis types 1 and 2 impaired in expression of muramidase-released protein and extracellular protein induce disease in newborn germfree pigs. Infect Immun. 1996;64(10):4409-12.

  35. Fittipaldi N, Fuller TE, Teel JF, Wilson TL, Wolfram TJ, Lowery DE, et al. Serotype distribution and production of muramidasereleased protein, extracellular factor and suilysin by fi eld strains of Streptococcus suis isolated in the United States. Vet Microbiol. 2009;139(3-4):310-7.

  36. Fittipaldi N, Xu J, Lacouture S, Tharavichitkul P, Osaki M, Sekizaki T, et al. Lineage and virulence of Streptococcus suis serotype 2 isolates from North America. Emerg Infect Dis. 2011;17(12):2239-44.

  37. De Greeff A, Buys H, Verhaar R, Van Alphen L, Smith HE. Distribution of environmentally regulated genes of Streptococcus suis serotype 2 among S. suis serotypes and other organisms. J Clin Microbiol. 2002;40(9):3261-8.




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