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2000, Número 5

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Arch Cardiol Mex 2000; 70 (5)


Dimorfismo sexual en el corazón de rata: Patrones diferenciales de las isoenzimas de la creatina cinasa catalíticamente activas

Ramírez OC, Jiménez E

Idioma: Ingles.
Referencias bibliográficas: 67
Paginas: 438-447
Archivo PDF: 303.49 Kb.


RESUMEN

Objetivos. Proponemos que en la rata, la síntesis anaeróbica de ATP mediada por el sistema creatina cinasa/fosfocreatina (CK/PCr) es sexualmente dimórfica durante la maduración y el envejecimiento del corazón. Antecedentes. En función de género, las diferencias morfológicas y funcionales durante el envejecimiento cardiovascular parecen explicar la mayor longevidad de las hembras de los mamíferos y de la mujer. aterial y Métodos. Se estudiaron 46 ratas Wistar de ambos sexos, por parejas de peso semejante de 200, 250 y 300 g de peso corporal. Resultados. No se observaron diferencias sexuales en cuanto al peso del corazón y a su contenido de proteínas del sobrenadante post 27 000 xg, en ninguno de los pesos corporales estudiados en la rata. Los cocientes peso cardíaco/peso corporal no mostraron diferencias significativas de género durante todo el estudio. Se encontraron diferencias de la actividad específica de la CK cardíaca solamente a los 257 ± 6 g de peso corporal, debido al decremento de tal actividad en el macho. El corazón de la hembra mostró una mayor variedad de isoenzimas de la CK citosólica en todos los pesos corporales estudiados. En los corazones de rata de ambos sexos se encontraron consistentemente isoenzimas del tipo cerebral BB-CK citosólicas fuertemente teñidas catalíticamente, durante todo el estudio. Este hallazgo no está de acuerdo con la aceptada especificidad tisular de la CK cardíaca. Conclusiones. En este trabajo se encontraron diferencias significativas de género, principalmente en cuanto a los patrones y al número de isoformas catalíticas de la CK citosólica del corazón de rata. En relación con los mecanismos anaeróbicos de la producción de ATP, estas diferencias podrían explicar, en parte, la susceptibilidad diferencial sexual al compromiso hemodinámico, en respuesta al estrés cardiovascular, en favor de las hembras.


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