Katz JMG, McReynolds S, Gardner DK, Schoolcraft WB

Idioma: Español
Referencias bibliográficas: 45
Paginas: 150-159
Archivo PDF: 159.47 Kb.

RESUMEN

La evaluación no invasiva de gametos y embriones se considera un aspecto importante en la tecnología de reproducción asistida. Actualmente, la selección de embriones para transferencia se basa en índices morfológicos. Aunque exitoso, el campo de la tecnología de reproducción asistida se beneficiaría de un método cuantitativo no invasivo para la determinación de la viabilidad. Las técnicas ómicas, incluidas la transcriptómica, proteómica y metabolómica, han empezado a proporcionar evidencia de que los gametos y embriones viables poseen perfiles moleculares únicos con biomarcadores potenciales que pueden utilizarse para la selección del desarrollo, de la viabilidad, o de ambas. A diferencia del genoma humano que es relativamente fijo y estable en todo el cuerpo humano, el proteoma humano, estimado en más de un millón de proteínas, es más complejo, diverso y dinámico. Las proteínas en sí mismas contribuyen a la homeostasia fisiológica de cualquier célula o tejido. De interés particular en la tecnología de reproducción asistida es el secretoma, las proteínas producidas dentro del embrión y secretadas en el ambiente circundante. La definición del secretoma embrionario humano tiene el potencial de expandir nuestro conocimiento de los procesos celulares embrionarios, incluido el complejo diálogo entre el embrión en desarrollo y su ambiente materno, y también puede ayudar en la identificación de los embriones con mayor potencial de implantación. Los avances en las técnicas proteómicas han permitido la caracterización no invasiva del secretoma embrionario humano con la investigación en curso enfocada en la correlación con el resultado. Desde una perspectiva clínica, la selección de embriones basada en la evaluación morfológica y el análisis no invasivo del secretoma embrionario humano puede aumentar el éxito de la FIV y resultar en la transferencia rutinaria de embriones individuales.

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