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2012, Número 1

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Biotecnol Apl 2012; 29 (1)


Estabilización de la glucosa oxidasa con carboximetilcelulosa ramificado con ciclodextrina

Matos M, Simpson BK, Ramírez HL, Cao R, Torres-Labandeira JJ, Hernández K

Idioma: Ingles.
Referencias bibliográficas: 34
Paginas:
Archivo PDF: 230.13 Kb.


RESUMEN

La glucosa oxidasa (GOx; EC 1.1.3.4) se modificó químicamente con el polímero carboximetilcelulosa ramificado con unidades de β ciclodextrina (CMC-CD) utilizando una carbodiimida como agente acoplante. La neoglicoenzima contenía 0.78 mol de polisacáridos por mol de GOx y retuvo, después de la transformación, el 67% de su actividad inicial. Se compararon algunas características de la enzima modificada y la nativa. La enzima modificada mostró valores de Km superiores a la enzima libre. La termoestabilidad de la glucosa oxidasa modificada con el polímero CMC-CD se incrementó de 45 ºC hasta 51 ºC. A través de esta metodología, se logró una mayor estabilidad de la enzima modificada con CMC-CD, la cual mostró 2.2 veces más resistencia a la inactivación térmica a 45 ºC. Por otra parte, este derivado retuvo el 75% de su actividad inicial después de tres horas de incubación lo cual demuestra una remarcada estabilidad en presencia del surfactante aniónico duodecilsulfato de sodio y reveló una máxima actividad a valores de pH alcalinos (7.5). La glicosilación covalente de la glucosa oxidasa con el polímero CMC-CD puede ser utilizada efectivamente para el mejoramiento de la estabilidad de esta enzima.


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