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2011, Número 3

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Rev Educ Bioquimica 2011; 30 (3)


F1F0-ATP Sintasa y sus diferencias estructurales

Cano-Estrada A, González-Halphen D

Idioma: Español
Referencias bibliográficas: 40
Paginas: 98-108
Archivo PDF: 1177.99 Kb.


RESUMEN

La estructura de la F1F0-ATPasa o F1F0-ATP sintasa es muy importante para entender su funcionamiento, por lo que ha sido de gran interés estudiar las subunidades que la componen, la interacción entre ellas y su función dentro de la enzima. La estructura de la F1F0-ATPasa varía de una especie a otra, encontrándose subunidades específicas de cada especie y siendo más compleja la estructura en los organismos más evolucionados; además estudios recientes han demostrado la presencia de subunidades novedosas dentro de la estructura de la ATP sintasa de algunas especies como las algas clorofíceas, el parásito Tripanosoma brucei y protistas como Tetrahymena thermophila. En este trabajo se pretende hacer una comparación de las subunidades que forman la F1F0-ATPasa de diferentes especies, haciendo hincapié en sus diferencias estructurales.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Alfonzo M, Kandrach MA, Racker E (1981) Isolation, characterization, and reconstitution of a solubilized fraction containing the hydrophobic sector of the mitochondrial proton pump. J Bioenerg Biomembr 13:375-391.

  2. Itoh H, Takahashi A, Adachi K, Noji H, Yasuda R, Yoshida M, Kinosita K (2004) Mechanically driven ATP synthesis by F1-ATPase. Nature 427:465-468.

  3. Weber J, Senior AE (2003) ATP synthesis driven by proton transport in F1F0-ATP synthase. FEBS Lett 545:61-70.

  4. Strauss M, Hofhaus G, Schröder RR, Kühlbrandt W (2008). Dimer ribbons of ATP synthase shape the inner mitochondrial membrane. EMBO J 27:1154-1160.

  5. Hausrath AC, Grüber G, Matthews BW, Capaldi RA (1999) Structural features of the gamma subunit of the Escherichia coli F(1) ATPase revealed by a 4.4-A resolution map obtained by x-ray crystallography. Proc Natl Acad Sci USA 96:13697–13702.

  6. Rodgers AJW, Wilce MCJ (2000) Structure of the γ–ε complex of ATP synthase. Nat Struct Biol 7:1051–1054.

  7. Tsunoda SP, Rodgers AJW, Aggeler R, Wilce MJC, Yoshida M, Capaldi RA (2001) Large conformational changes of the epsilon subunit in the bacterial F1F0 ATP synthase provide a ratchet action to regulate this rotary motor enzyme. Proc Natl Acad Sci USA 98:6560-6564.

  8. Fillingame RH, Jiang W, Dmitriev OY (2000) The oligomeric subunit C rotor in the Fo sector of ATP synthase: unresolved questions in our understanding of function. J Bioenerg Biomembr 32:433-439.

  9. Jiang W, Hermolin J, Fillingame RH (2001) The preferred stoichiometry of c subunits in the rotary motor sector of Escherichia coli ATP synthase is 10. Proc Natl Acad Sci USA 98:4966-4971.

  10. Wilkens S, Capaldi RA (1998) ATP synthase´s second stalk comes into focus. Nature 393:29.

  11. Dmitriev O, Jones PC, Jiang W, Fillingame RH (1999) Structure of the membrane domain of subunit b of the Escherichia coli F0F1 ATP synthase. J. Biol. Chem. 274:15598-15604.

  12. Wilkens S, Borchardt D, Weber J, Senior AE (2005) Structural Characterization of the interaction of the δ and a subunits of the Escherichia Coli F1F0-ATP synthase by NMR spectroscopy. Biochemistry 44:11786-11794.

  13. Collinson IR, van Raaij MJ, Runswick MJ, Fearnley IM, Skehel JM, Orriss GL, Miroux B, Walker JE (1994) ATP synthase from bovine heart mitochondria. In vitro assembly of stalk complex in the presence of F1-ATPase and in its absence. J Mol Biol 242:408-421.

  14. Dickson VK, Silvester JA, Fearnley IM, Leslie AGW, Walker JE (2006) On the structure of the stator of the mitochondrial ATP synthase. EMBO J 25:2911-2918.

  15. Rees DM, Leslie AG, Walker JE (2009) The structure of the membrane extrinsic region of bovine ATP synthase. Proc Natl Acad Sci USA 106:21597–21601.

  16. Vaillier J, Arselin G, Graves PV, Camougrand N, Velours J (1999) Isolation of supernumerary yeast ATP synthase subunits e and i. Characterization of subunit i and disruption of its structural gene ATP18. J Biol Chem 274:543-8.

  17. Paumard P, Vaillier J, Coulary B, Schaeffer J, Soubannier V, Mueller DM, Brethes D, di Rago JP, Velours J (2002) The ATP synthase is involved in generating mitochondrial cristae morphology. EMBO J 21:221-230.

  18. Sabbar S, Stuart RA (2005) The yeast F1F0-ATP Synthase. Analysis of the molecular organization of subunit g and the importance of a conserved GXXXG motif. J Biol Chem 280:24435-24442.

  19. Arnold I, Pfeiffer K, Neupert W, Stuart RA, Schägger H (1998) Yeast mitochondrial F1F0-ATP synthase exists as a dimer: identification of three dimer-specific subunits. EMBO J 17:7170-7178.

  20. Cabezón E, Montgomery MG, Leslie AGW and Walker JE (2003) The structure of bovine F1- ATPase in complex with its regulatory protein IF1. Nat Struct Biol 10:744-750.

  21. Ichikawa N, Yoshida Y, Hashimoto T, Ogasawara N, Yoshikawa H, Imamoto F and Tagawa K (1990) Activation of ATP hydrolysis by an uncoupler in mutant mitochondria lacking an intrinsic ATPase inhibitor in yeast. J Biol Chem 265:6274–6278.

  22. Belogrudov GI, Hatefi Y (2002) Factor B and the mitochondrial ATP synthase complex. J Biol Chem 277:6097-6103.

  23. Brugière S, Kowalski S, Ferro M, Seigneurin- Berny D, Miras S, Salvi D, Ravanel S, d’Hérin P, Garin J, Bourguignon J, Joyard J, Rolland N (2004) The hydrophobic proteome of mitochondrial membranas from Arabidopsis cell suspensions. Phytochemistry 65:1693-1707.

  24. Heazlewood JL, Whelan J, Millar AH (2003) The products of the mitochondrial orf25 and orfB genes are F0 components in the plant F1F0 ATP synthase. FEBS Lett 540:201-205.

  25. Gray MW, Lang BF, Cedergren R, Golding GB, Lemieux C, Sankoff D, Turmel M, Brossard N, Delage E, Littlejohn TG, Plante I, Rioux P, Saint-Louis D, Zhu Y, Burger G (1998) Genome structure and gene content in protist mitochondrial DNAs. Nucleic Acids Res 26:865-878.

  26. Tang HV, Pring DR, Muza FR and Yan B (1996) Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm. Curr Gen 29:265-274.

  27. Nurani G, and Franzén LG (1996) Isolation and characterization of the mitochondrial ATP synthase from Chlamydomonas reinhardtii. cDNA sequence and deduced protein sequence of the alpha subunit. Plant Mol Biol 6:1105-1116.

  28. Franzén LG, Falk G (1992) Nucleotide sequence of cDNA clones encoding the beta subunit of mitochondrial ATP synthase from the green alga Chlamydomonas reinhardtii: the precursor protein encoded by the cDNA contains both an N-terminal presequence and a C-terminal extension. Plant Mol Biol 19: 771-780.

  29. Villavicencio-Queijeiro A, Vázquez-Acevedo M, Cano-Estrada A, Zarco-Zavala M, Tuena de Gómez M, Mignaco JA, Freire MM, Scofano Helena M, Foguel D, Cardol P. Remacle C, González-Halphen D (2009) The fully-active and structurally-stable form of the mitochondrial ATP synthase of polytomella sp. is dimeric. J Bioenerg Biomembr 41:1–13.

  30. Funes S, Davidson E, Claros MG, van Lis R, Pérez-Martínez X, Vázquez- Acevedo M, King MP, González-Halphen D (2002) The typically mitochondrial DNA-encoded ATP6 subunit of the F1F0-ATPase is encoded by a nuclear gene in Chlamydomonas reinhardtii. J Biol Chem 277:6051–6058.

  31. Cardol P, Gonzalez-Halphen D, Reyes-Prieto A, Baurain D, Matagne RF, Remacle C (2005) The mitochondrial oxidative phosphorylation proteome of Chlamydomonas reinhardtii deduced from the Genome Sequencing Project. Plant Physiol 137:447-459.

  32. Vázquez-Acevedo M, Cardol P, Cano-Estrada A, Lapaille M, Remacle C, González- Halphen D (2006) The mitochondrial ATP synthase of chlorophycean algae contains eight subunits of unknown origin involved in the formation of an atypical stator-stalk and in the dimerization of the complex. J Bioenerg Biomem 38:271-282.

  33. Cano Estrada A, Vazquez-Acevedo M, Villavicencio-Queijeiro A, Figueroa-Martinez F, Miranda-Astudillo H, Cordeiro Y, Mignaca JA, Foguel D, Cardol P, Lapaille M, Remacle C, Wilkens S, and Gonzalez-Halphen D (2010) Subunits-Subunits interations and overall topology of the dimeric mitochondrial ATP synthase of Polytomella sp. Biochim Biophys Acta 1797:1439-1448.

  34. Van Lis R, Atteia A, Mendoza-Hernández G, González-Halphen D (2003) Identification of 21 novel mitochondrial protein components of Chlamydomonas reinhardtii: A proteomic approach. Plant Physiol 132:318-330.

  35. Dudkina NV, Heinemeyer J, Keegstra W, Boekema EJ, Braun HP (2005) Structure of dimeric ATP synthase from mitochondria: an angular association of monomers induces the strong curvature of the inner membrane. FEBS Lett 579: 5769-5772.

  36. Van Lis R, Mendoza-Hernández G, Groth G, Atteia A (2007) New insights into the unique structure of the F0F1-ATP synthase from the chlamydomonad algae Polytomella sp. and Chlamydomonas reinhardtii. Plant Physiol 144:1190-1199.

  37. Lapaille M, Escobar-Ramirez A, Degand H, Baurain D, Rodriguez-Salinas E, Coosemans N, Boutry M, Gonzálex-Halphen D, Remacle C, Cardol P (2010) Atypical subunit composition of the chlorophycean mitochondrial F1F0 ATP synthase and role of ASA7 protein in stability and aligomycin resistance of the enzyme. Mol Biol Evol 27:1630-1644.

  38. Dudkina NV, Sunderhaus S, Braun HP, Boekema EJ (2006) Characterization of dimeric ATP synthase and cristae membrane ultrastructure from Saccharomyces and Polytomella mitochondria. FEBS Lett 580:3427-3432.

  39. Zíková A, Schnaufer A, Dalley RA, Panigrahi AK, Stuart KD (2009) The F0F1-ATP Synthase complex contains novel subunits and is essential for Procyclic Trypanosoma brucei. PLoS Pathogens 5:1-15.

  40. Balabaskaran NP, Dudkina NV, Kane LA, van Eyk JE, Boekema EJ, Mather MW, Vaidya AB (2010) Highly divergent mitochondrial ATP synthase complexes in Tetrahymena thermophila. Plos Biol 7:e1000418.




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