Bolado-Martínez E, Pérez-Mendoza A, Alegría-Morquecho FM, Candia-Plata MC, Aguayo-Verdugo MR, Álvarez-Hernández G

Idioma: Español
Referencias bibliográficas: 16
Paginas: 167-170
Archivo PDF: 93.08 Kb.

RESUMEN

Objetivo. Realizar el análisis de regiones específicas de genes asociados con resistencia a isoniazida o rifampicina. Material y métodos. Se estudiaron 22 cepas de M. tuberculosis, aisladas en Sonora, México. Se utilizaron iniciadores para regiones específicas de los genes rpoB, katG e inhA y la región ahpC-oxyR. Los productos de PCR se secuenciaron y analizaron. Resultados. Se identificaron mutaciones en la región promotora del gen inhA, región ahpC-oxyR, codón 315 del gen katG y codones 451 ó 456 del gen rpoB. Conclusiones. La identificación de mutaciones no descritas previamente obliga a continuar el análisis genotípico de cepas aisladas en Sonora.

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