Hernández-Saavedra D, McCord J

Idioma: Español
Referencias bibliográficas: 28
Paginas: 533-538
Archivo PDF: 184.13 Kb.

RESUMEN

Introducción: proponemos que un estado oxidativo moderado induce a las células a proliferar, sin embargo, un aumento descontrolado del nivel oxidativo puede causar la muerte o daños irreversibles de las células. Ejemplos de respuestas fisiológicas no balanceadas al anión superóxido son la proliferación no regulada en estados patológicos como cáncer y la destrucción de células β en diabetes.
Métodos: se utilizaron mitógenos para inducir activación de linfocitos de sangre periférica tales como fitohemaglutinina, concanavalina A y ésteres de forbol. Se utilizó también paraquat como inductor de la generación del anión superóxido. Las respuestas fisiológicas fueron evaluadas en términos de proliferación celular utilizando MTT, así como también la muerte celular por apoptosis y necrosis.
Resultados: el incremento en el estado oxidativo de linfocitos en reposo induce proliferación celular, pero altas concentraciones de superóxido son tóxicas e inducen apoptosis y necrosis.
Conclusiones: nuestros resultados muestran la participación del anión superóxido en respuestas fisiológicas celulares como proliferación y apoptosis. Estas respuestas divergentes pueden explicarse en términos de nuestras observaciones de dosis-respuesta que ejemplifican la importancia del balance oxidativo.

REFERENCIAS (EN ESTE ARTÍCULO)

1. Seve M, Favier A, Osman M, Hernández D, Vaitaitis G, Flores NC, McCord JM, Flores SC. The human immunodeficiency virus-1 Tat protein increases cell proliferation, alters sensitivity to zinc chelator-induced apoptosis, and changes Sp1 DNA binding in HeLa cells. Arch Biochem Biophys 1999;361:165-172.

2. Hernández-Saavedra D, McCord JM. Paradoxical effects of thiol reagents on Jurkat cells and a new thiolsensitive form of human mitochondrial superoxide dismutasa. Cancer Res 2003;63: 159-163.

3. Janssen YMW, Vanhouten B, Borm PJA, Mossman BT. Cell and tissue responses to oxidative damage. Lab Invest 1993;69:261-274.

4. McCord JM. Superoxide radical: controversies, contradictions, and paradoxes. Proc Soc Exp Biol Med 1995; 209:112-117.

5. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2):55-63.

6. Loveland BE, Johns TG, Mackay IR, Vaillant F, Wang ZX, Hertzog PJ. Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays. Biochem Int 1992;27 (3):501-510.

7. Melinn M, McLaughlin H. Nitroblue tetrazolium reduction in lymphocytes. J Leukoc Biol 1987;41: 325-329.

8. Schreck R, Rieber P, Baeuerle PA. Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa β transcription factor and HIV-1. EMBO J 1991;10:2247-2258.

9. Tatla S, Woodhead V, Foreman JC, Chain BM. The role of reactive oxygen species in triggering proliferation and IL-2 secretion in T cells. Free Radic Biol Med 1999;26:14-24.

10. Khan MA, Jeremy JY, Hallinan T, Tateson JE, Hoffbrand AV, Wickremasinghe RG. Antioxidants impair the coupling of cell-surface ligand receptors to the inositol lipid signalling pathway in human T lymphocytes but not in Jurkat T lymphoblastic leukemia cells. Evidence that leukotrienes are not involved in the coupling mechanism. Biochim Biophys Acta 1993;1178:215-220.

11. Richter C. Pro-oxidants and mitochondrial Ca2 +: their relationship to apoptosis and oncogenesis. FEBS Lett 1993;325:104-107.

12. Buttke TM, Sandstrom PA. Oxidative stress as a mediator of apoptosis. Immunol. Today 1994;15:7-10.

13. Lennon SV, Martin SJ, Cotter TG. Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell Prolif 1991; 24:203-214.

14. Bazar LS, Deeg HJ. Ultraviolet B-induced DNA fragmentation (apoptosis) in activated T-lymphocytes and Jurkat cells is augmented by inhibition of RNA and protein synthesis. Exp Hematol 1992; 20:80-86.

15. Seve M, Favier A, Osman M, Hern¨¢ndez D, Vaitaitis G, Flores NC, McCord JM, Flores SC. The human immunodeficiency virus-1 Tat protein increases cell proliferation, alters sensitivity to zinc chelator-induced apoptosis, and changes Sp1 DNA binding in HeLa cells. Arch Biochem Biophys 1999;361:165-172.

16. Hern¨¢ndez-Saavedra D, McCord JM. Paradoxical effects of thiol reagents on Jurkat cells and a new thiolsensitive form of human mitochondrial superoxide dismutasa. Cancer Res 2003;63: 159-163.

17. Janssen YMW, Vanhouten B, Borm PJA, Mossman BT. Cell and tissue responses to oxidative damage. Lab Invest 1993;69:261-274.

18. McCord JM. Superoxide radical: controversies, contradictions, and paradoxes. Proc Soc Exp Biol Med 1995; 209:112-117.

19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65(1-2):55-63.

20. Loveland BE, Johns TG, Mackay IR, Vaillant F, Wang ZX, Hertzog PJ. Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays. Biochem Int 1992;27 (3):501-510.

21. Melinn M, McLaughlin H. Nitroblue tetrazolium reduction in lymphocytes. J Leukoc Biol 1987;41: 325-329.

22. Schreck R, Rieber P, Baeuerle PA. Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa β transcription factor and HIV-1. EMBO J 1991;10:2247-2258.

23. Tatla S, Woodhead V, Foreman JC, Chain BM. The role of reactive oxygen species in triggering proliferation and IL-2 secretion in T cells. Free Radic Biol Med 1999;26:14-24.

24. Khan MA, Jeremy JY, Hallinan T, Tateson JE, Hoffbrand AV, Wickremasinghe RG. Antioxidants impair the coupling of cell-surface ligand receptors to the inositol lipid signalling pathway in human T lymphocytes but not in Jurkat T lymphoblastic leukemia cells. Evidence that leukotrienes are not involved in the coupling mechanism. Biochim Biophys Acta 1993;1178:215-220.

25. Richter C. Pro-oxidants and mitochondrial Ca2+: their relationship to apoptosis and oncogenesis. FEBS Lett 1993;325:104-107.

26. Buttke TM, Sandstrom PA. Oxidative stress as a mediator of apoptosis. Immunol. Today 1994;15:7-10.

27. Lennon SV, Martin SJ, Cotter TG. Dose-dependent induction of apoptosis in human tumour cell lines by widely diverging stimuli. Cell Prolif 1991; 24:203-214.

28. Bazar LS, Deeg HJ. Ultraviolet B-induced DNA fragmentation (apoptosis) in activated T-lymphocytes and Jurkat cells is augmented by inhibition of RNA and protein synthesis. Exp Hematol 1992; 20:80-86.