Hernández VG, Ramírez RR, Rodríguez TLE, Mora VFA, Vidales CJA, Hernández EJJ

Idioma: Español/Inglés
Referencias bibliográficas: 46
Paginas: 239-249
Archivo PDF: 142.04 Kb.

RESUMEN

Se identificó la microflora normal, formada por hongos, levaduras y bacterias, de la conjuntiva en 50 caballos clínicamente sanos, de diferente raza y sexo, de tres a 12 años de edad, del área metropolitana de Monterrey, Nuevo León. Las muestras se tomaron del saco conjuntival de ambos ojos (n = 100 ojos) con un hisopo bajo condiciones estériles. Ningún ojo fue negativo a la presencia de microorganismos. Hubo a menudo diferencias en los tipos de colonias entre los ojos del mismo animal, 100% fueron positivos a la presencia de hongos y bacterias en ambos ojos, y 60% positivos a la presencia de levaduras. Este estudio presenta, por vez primera en conjuntiva de caballos, la presencia de Serratia marcescens, considerada microorganismo patógeno oportunista con multirresistencia a fármacos. En esta investigación, Serratia marcescens se logró aislar de 21 caballos clínicamente sanos (42%) con rango de nueve a 42 unidades formadoras de colonias (ufc).

REFERENCIAS (EN ESTE ARTÍCULO)

1. BURNS RP. Indigenous flora of the lids and conjunctiva. In: TASMAN W, JAEGER EA, editors. Foundations of clinical ophthalmology. Vol 2. Philadelphia:. Lippincott Press, 1993:1-5.

2. SPRADBROW PB. The bacterial flora of the ovine conjunctival sac. Aust Vet J 1968; 44:117-118.

3. SAMUELSON DA, ANDRESEN TL, GWIN RM. Conjunctival fungal flora in horses, cattle, dogs and cats. J Am Vet Med Assoc 1984; 184:1240-1242.

4. WILCOX GE. Bacterial flora of the bovine eye with special reference to the Moraxella and Neisseria. Aust Vet J1970; 46:253-256.

5. DAVISON HJ, ROGERS DP, YEARY TJ, STONE GG, SCHONEWESIS DA, CHENGAPPA MM. Conjunctival microbial flora of clinically normal pigs. Am J Vet Res 1994; 55:949-951.

6. DUPONT C, CARRIER M, HIGGINS R. Bacterial and fungal in healthy eyes of birds of pray. Can Vet J 1994; 34:699-701.

7. COOPER SC, MCLELLAN GJ, REYCROFT AN. Conjunctival flora observed in 70 healthy domestic rabbits. Vet Rec 2001; 149:232-235.

8. 8.MUSHI EZ, BINTA MG, CHAVO RG, DINTWE K. Conjunctival flora of fifty healthy goats in Sebele farm, Gaborone, Botswana. J Anim Vet Adv 2007; 6:1388-1389.

9. KUDIRKIENÈ E, ZILINSKA H, SIUGZDAITE J. Microbial flora of the dog eyes. Lithuanian Vet Acad 2006; 34:18-21.

10. TUNTIVANICHP, SOONTORNIPAVRT K, TUNTIVANICH N, WONGAUMNUAYKUL S, BRIKSAWAN P. Conjunctival microflora in clinically normal Asian elephant in Thailand. Vet Res Comm 2002; 26:251-254.

11. SHEWEN PE, POVERY RC, WILSON MR. A survey of conjunctival flora of clinically normal cats and cats with conjunctivitis. Can Vet J 1980; 21:231-233.

12. NARDONI S, SGORBINI M, BARSOTTI M, CORAZZA G, MANCIATTI F. Conjunctival fungal flora in healthy donkeys. Vet Ophthalmol 2007; 10:207-210.

13. ANDREW SE, NGUYEN A, JONES GL, BROOKS DE. Seasonal effects on the aerobic bacterial and fungal conjunctival flora of normal thoroughbred brood mares in Florida. Vet Ophthalmol 2003; 6:45-50.

14. ROSA M, CARDOZO LM, PEREIRA JDAS, BROOKS DE, MARTINS ALB, FLORIDO PSS et al. Fungal flora of normal eyes of healthy horses from the state of Rio de Janeiro, Brazil. Vet Ophthalmol 2003; 6:51-55.

15. MOORE C, HELLER N, MAJORS LJ, WHITLEY RD, BURGUÉS EC, WEBER J. Prevalence of ocular microorganisms in hospitalized and stabled horses. Am J Vet Res 1988; 49:773-777.

16. GELATT KN. Fundamentos de Oftalmología Veterinaria. Barcelona: MASSON SA, 2003.

17. ARGUESO P. Azúcares: Una capa protectora excepcional de la superficie ocular. Arch Soc Esp Oftalmol 2008; 83:287-290.

18. MARTOS PG, FERNÁNDEZ DEL BARRIO MT, SALIDO FPF. Microbiología Clínica Aplicada. 3a ed. Madrid, España: Ed. Díaz de Santos SA, 1997.

19. KONEMAN EW, ALLEN SD, DOWELL VR, SOMMERS HM. Diagnóstico microbiológico. Madrid, España: Ed. Médica Panamericana SA, 1989.

20. BARNETT H, HUNTER BB. Illustrated genera of imperfect fungi. 3rd ed. Minneapolis Minnesota USA: Burgess Publishing Company, 1972.

21. FISHER F, COOK NB. Fundamental of Diagnostic Mycology. Pennsylvania: WB Saunders Company, 1998.

22. DE HOOG GS, GUARRO J, GENÉ J, FIGUERAS MJ. Atlas of Clinical Fungi. 2nd ed. Centralbureau voor Schimmelcultures, Utrecht, The Netherlands/ Universitat Rovira i Virgili, Reus, Spain, 2000.

23. 23.RALLIS E, KARANIKOLA E, PAPADAKIS P. Severe facial infection caused by Serratia marcescens in an immunocompetent soldier. J Am Acad Dermatol 2008; 58:109-110.

24. EQUI RA, GREEN WR. Endogenous Serratia marcescens endophthalmitis with dark hypopyon: a case report and review. Surv Ophthalmol 2001; 46:259-268.

25. SHARMA NS, OOI JL, DOWNIE JA, CORONEO MT. Corneal perforation and intraocular lens prolapsed in Serratia marscescens endophthalmitis. Clin Experiment Ophthalmol 2007; 35:381-382.

26. ALEXANDRAKIS G, ALFONSO EC, MILLAR D. Shifting trends in bacterial keratitis in south Florida and emerging resistance to fluoroquinolones. Ophthalmology 2000; 107:1497-1502.

27. LYERLY D, GRAY L, KREGER A. Characterization of rabbit corneal damage produced by Serratia keratitis and by Serratia protease. Infect Immun 1981; 33:927- 932.

28. FOX JM, BEAUCAGE CM, FOLTA CA, TROTON GW. Nosocomial transmission of Serratia marcescens in a veterinary hospital due to contamination by benzalkonium chloride. J Clin Microbiol 1981; 14:157-160.

29. KAMARUDIN MI, FOX LK, GASKINS CT, GAY JM. Environmental reservoirs for Serratia marscescens intramammary infections in dairy cows. J Am Vet Med Assoc 1966; 208:555-558.

30. EWART S, BROWN C, DERKSEN F, KUFUOR-MENSA E. Serratia marcescens endocarditis in a horse. J Am Vet Med Assoc 1992; 200:961-963.

31. SHAFTOE S. Serratia marscescens septicaemia in a neonatal Arabian foal. Equine Vet J 1984; 16:389-392.

32. RIGG DL, GARTLIN SJ, REINERTSON EL. Marsupialization of an abdominal abscess caused by Serratia marscescens in a mare. J Am Vet Med Assoc 1987; 191:222-224.

33. JORES J, BEUTER G, HIRTH-SCHMIDT I, BORSHERS K, PITT TL, LUBKE-BECKER A. Isolation of Serratia marcescens from an equine abortion in Germay. Vet Rec 2004; 154:242-244.

34. GEMENSKY-METZLER AJ, WILKIE DA, KOWALSKI JJ, SCHMALL LM, WILLIS AM, YAMAGATA M. Changes in bacterial and fungal ocular flora of clinically normal horses following experimental application of topical antimicrobial or antimicrobial-corticosteroid ophthalmic preparations. Am J Vet Res 2005; 66:800-811.

35. ZENOBLE RD, GRIFFITH RW, CLUBB SL. Survey of bacteriologic flora of conjunctiva and cornea in healthy psittacine birds. Am J Vet Res 1983; 44:1966-1967.

36. WOLF ED, AMASS K, OLSEN J. Survey of conjunctival flora in the eye of clinically normal captive exotic birds. J Am Med Vet Assoc 1983; 183:1232-1233.

37. MCDONALD PJ, WATSON DJ. Microbial flora of normal canine conjunctivae. J Small Anim Pract 1976;17:809-812.

38. ESPINOLA MB, LILENBAUM W. Prevalence of bacteria in the conjunctival sac and on the eyelid margin of clinically normal cats. J Small Anim Pract 1996; 37:364-366.

39. BARNETT KC, CRISPIN SM, LAVACH JD, MATTHEWS AG. Equine ophthalmology. 2nd ed. Philadelphia, USA: Elsevier Limited, 2004.

40. SANSON J, FEATHERSTONE H, BARNETT KC. Keratomycosis in six horses in the United Kindgdom. Vet Rec 2005; 156:13-17.

41. MATTHEWS AG. The aethiopatogenesis of infectious keratitis in the horse. Equine Vet J 1994; 26:432-433.

42. BARSOTTI G, SGORBINI M, NARDONI S, CORAZZA M, MANCIANTI F. Occurrence of fungi from conjunctiva of healthy horses in Tuscany, Italy. Vet Res Commun 2006; 30: 903-906.

43. WESTEIN WL, MOORE PA, SANCHEZ S, DIETRICH UM, WOOLEY RE, RITCHIE BW. In vitro efficacy of a buffered chelating solution as an antimicrobial potentiator for antifungal drugs against pathogens obtained from horses with mycotic keratitis. Am J Vet Res 2006; 67:562-568.