Velázquez OV, Pescador SN, Gorodezky C, Saltijeral OJ

Idioma: Ingles.
Referencias bibliográficas: 48
Paginas: 66-71
Archivo PDF: 62.25 Kb.

RESUMEN

Se evaluó la diferencia entre los neutrófilos (PMN) de sangre y leche para fagocitar in vitro Staphylococcus aureus marcados con fluoresceína en 14 vacas lecheras clínicamente sanas durante 4-6 semanas. La concentración de PMN de sangre se realizó por lisis hipotónica de eritrocitos (HEL) y en leche mediante perfusión intramamaria de lipopolisacárido B de Escherichia coli 0:26:B6 (LPS). El índice de fagocitosis in vitro fue mayor en los PMN de sangre 7.34 ± 1.55 comparados con las células en leche 4.71 ± 1.31 (P ‹ 0.001). La capacidad de fagocitosis de los PMN de sangre fue 86.5 ± 9.6%, comparada con una capacidad reducida de las células en leche (P ‹ 0.01). Se observó una relación lineal en la actividad de fagocitosis de las células de sangre y leche (y = 0.84 x+ 3.37; R² = 0.3783), con una asociación (r = 0.615). La concentración de PMN es útil y reproducible para ensayos de fagocitosis in vitro. Los hallazgos son consistentes con la capacidad de respuesta de la glándula mamaria, al inducir una rápida migración de PMN de la sangre frente a la infección. El procedimiento descrito puede facilitar la evaluación de la respuesta inicial de la respuesta inmune innata en las vacas lecheras.

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